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Species: Arabidopsis thaliana
Genetic Insert: ABI2
Vector Backbone Description: Backbone Marker:Toulouse; Vector Backbone:pAMPAT split-YFP; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_102383 Copy
Species: Arabidopsis thaliana
Genetic Insert: PBL27
Vector Backbone Description: Backbone Marker:Toulouse; Vector Backbone:pAMPAT split-YFP; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_102388 Copy
Species: Arabidopsis thaliana
Genetic Insert: CBL4
Vector Backbone Description: Backbone Marker:Toulouse; Vector Backbone:pAMPAT split-YFP; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_102396 Copy
Species: Arabidopsis thaliana
Genetic Insert: CIPK5
Vector Backbone Description: Backbone Marker:Toulouse; Vector Backbone:pAMPAT split-YFP; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_102392 Copy
Species: Arabidopsis thaliana
Genetic Insert: CBL1
Vector Backbone Description: Backbone Marker:Toulouse; Vector Backbone:pAMPAT split-YFP; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_102393 Copy
Species: Arabidopsis thaliana
Genetic Insert: CBL9
Vector Backbone Description: Backbone Marker:Toulouse; Vector Backbone:pAMPAT split-YFP; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_102400 Copy
Species: Arabidopsis thaliana
Genetic Insert: RabG3B
Vector Backbone Description: Vector Backbone:pUBN-RFP; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Proper citation: RRID:Addgene_102371 Copy
Species: Arabidopsis thaliana
Genetic Insert: RabG3B
Vector Backbone Description: Backbone Marker:Toulouse; Vector Backbone:pAMPAT split-YFP; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_102372 Copy
Species: Arabidopsis thaliana
Genetic Insert: EFR
Vector Backbone Description: Vector Backbone:pEarleyGate103; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_102413 Copy
Species: Arabidopsis thaliana
Genetic Insert: Phytochrome B (1-621 aa)
Vector Backbone Description: Backbone Marker:Junichi Miyazaki (Osaka University, Japan); Backbone Size:4870; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29078307
Comments: Expression vector of PhyB621-mCherry-HRasCT in mammalian cells. Of note, the expression level of PIF3-mEGFP must be comparable or lower than that of PhyB. We usually co-transfect pCAGGS-PhyB621-mCherry-HRasCT and pCAGGS-PIF3-mEGFP plasmids into HeLa cells with lipofection in a 50:1 ratio.
mCherry has a G229L mutation that does not affect function.
Proper citation: RRID:Addgene_100282 Copy
Species: Arabidopsis thaliana
Genetic Insert: Phytochrome interacting factor 3
Vector Backbone Description: Backbone Marker:Junichi Miyazaki (Osaka University, Japan); Backbone Size:4870; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29078307
Comments: Expression vector of PIF3-mEGFP in mammalian cells. Of note, the expression level of PIF3-mEGFP must be comparable or lower than that of PhyB. We usually co-transfect pCAGGS-PhyB-mCherry-HRasCT and pCAGGS-PIF3-mEGFP plasmids into HeLa cells with lipofection in a 50:1 ratio.
Proper citation: RRID:Addgene_100283 Copy
Species: Arabidopsis thaliana
Genetic Insert: Phytochrome B
Vector Backbone Description: Backbone Size:4885; Vector Backbone:pL1A0*B0*_Leu; Vector Types:Yeast Expression, Cre/Lox, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29789561
Proper citation: RRID:Addgene_100538 Copy
Species: Arabidopsis thaliana
Genetic Insert: OEP7(1–50)
Vector Backbone Description: Vector Backbone:pDONR207; Vector Types:Gateway Cloning; Bacterial Resistance:Gentamicin
Defining Citation: PMID:28634853
Proper citation: RRID:Addgene_100601 Copy
Species: Arabidopsis thaliana
Genetic Insert: Phytochrome B (1-908 aa, Y276H mutation)
Vector Backbone Description: Backbone Marker:Junichi Miyazaki (Osaka University, Japan); Backbone Size:4870; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29078307
Comments: Expression vector of PhyB-Y276H-mCherry-HRasCT in mammalian cells.
PhyB-Y276H has two additional missense mutations (P840S, P884L); the construct will function as described in the publication.
mCherry has a G229L mutation that does not affect function.
Proper citation: RRID:Addgene_100540 Copy
Species: Arabidopsis thaliana
Genetic Insert: PEROXISADE36
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5892; Vector Backbone:pDONR_P4-P1R; Vector Types:Gateway promoter entry clone; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31422517
Comments: For use with Three-way Multi-site Gateway Cloning (Invitrogen).
Proper citation: RRID:Addgene_128431 Copy
Species: Arabidopsis thaliana
Genetic Insert: Mpdelta
Vector Backbone Description: Backbone Marker:Cambia; Vector Backbone:pMOD_D4800EC; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31844292
Proper citation: RRID:Addgene_127231 Copy
Species: Arabidopsis thaliana
Genetic Insert: STM
Vector Backbone Description: Backbone Marker:Cambia; Vector Backbone:pMOD_D4800EC; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31844292
Proper citation: RRID:Addgene_127228 Copy
Species: Arabidopsis thaliana
Genetic Insert: EGFP reverse complement coding sequence flanked by attB/attP Integrase 13 attachment sites.
Vector Backbone Description: Vector Backbone:pUC18; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32444777
Comments: The whole insert (promoter, CDS and terminator) was synthesized and cloned into backbone plasmid. The CaMV 35S promoter sequence is derived from iGEM registry (BBa_K1547006); the EGFP coding sequence from Chiu, W. et al. Curr. Biol. 6, 325-330 (1996); the attB/P integrase 13 attachment sites sequences from Yang, L. et al. Nat. Methods 11, 1261-1266 (2014); and the NOS terminator sequence from pBI426 plasmid of Datla, R.S., et al. Gene 101, 239-246 (1991).
Proper citation: RRID:Addgene_127526 Copy
Species: Arabidopsis thaliana
Genetic Insert: EGFP reverse complement coding sequence flanked by attB/attP Integrase 9 attachment sites.
Vector Backbone Description: Vector Backbone:pUC18; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32444777
Comments: The whole insert (promoter, CDS and terminator) was synthesized and cloned into backbone plasmid. The CaMV 35S promoter sequence is derived from iGEM registry (BBa_K1547006); the EGFP coding sequence from Chiu, W. et al. Curr. Biol. 6, 325-330 (1996); the attB/P integrase 9 attachment sites sequences from Yang, L. et al. Nat. Methods 11, 1261-1266 (2014); and the NOS terminator sequence from pBI426 plasmid of Datla, R.S., et al. Gene 101, 239-246 (1991).
Proper citation: RRID:Addgene_127525 Copy
Species: Arabidopsis thaliana
Genetic Insert: EGFP reverse complement coding sequence flanked by attB/attP Integrase 5 attachment sites.
Vector Backbone Description: Vector Backbone:pUC18; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32444777
Comments: The whole insert (promoter, CDS and terminator) was synthesized and cloned into backbone plasmid. The CaMV 35S promoter sequence is derived from iGEM registry (BBa_K1547006); the EGFP coding sequence from Chiu, W. et al. Curr. Biol. 6, 325-330 (1996); the attB/P integrase 5 attachment sites sequences from Yang, L. et al. Nat. Methods 11, 1261-1266 (2014); and the NOS terminator sequence from pBI426 plasmid of Datla, R.S., et al. Gene 101, 239-246 (1991).
Proper citation: RRID:Addgene_127523 Copy
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