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Species: Drosophila melanogaster
Genetic Insert: DmDCP1
Vector Backbone Description: Vector Backbone:pMK33-NTAP; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Comments: pMK33-NTAP and pMK33-CTAP vectors are designed for making proteinfusions tagged with the TAP tag and for generating stableDrosophila cell lines under Hygromycin selection. TAP fusions areinducible with copper sulfate. The parental vector, pMK33 (pMt/Hy),was created by Michael Koelle. Sequence based on the sequence given by NCBI: gi: 22026940. Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_146735 Copy
Species: Drosophila melanogaster
Genetic Insert: DmDCP1-R57D-dsRNAres
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Comments: ORF extracted from NCBI: NM_137998.2 Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_147268 Copy
Species: Drosophila melanogaster
Genetic Insert: DmDCP1-F36A-dsRNAres
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Comments: ORF extracted from NCBI: NM_137998.2 Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_147266 Copy
Species: Drosophila melanogaster
Genetic Insert: DmDCP1-R96AS100A-dsRNAres
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Comments: ORF extracted from NCBI: NM_137998.2 Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_147270 Copy
Species: Drosophila melanogaster
Genetic Insert: DmDCP2-E361A-dsRNAres
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Comments: sequence given by NCBI: NM_140548.3 Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_146630 Copy
Species: Drosophila melanogaster
Genetic Insert: DmDCP1_1-127
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23142987
Comments: Sequence based on the sequence given by NCBI: gi: 22026940. Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_147050 Copy
Species: Drosophila melanogaster
Genetic Insert: OSS Shu N-terminal 3xFlag-3xHA Tag Donor Plasmid
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35639929
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.12.18.423517v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_186655 Copy
Species: Drosophila melanogaster
Genetic Insert: Piwi sgRNA Plasmid
Vector Backbone Description: Vector Backbone:pU6-BbsI-chiRNA; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35639929
Comments: sgRNA sequenceGCGAGTGCCAAAAAGTAACAA, The corresponding genome sequence is: GCGAGTGCCAAAAAGTAACA.
Please visit https://www.biorxiv.org/content/10.1101/2020.12.18.423517v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_186653 Copy
Species: Drosophila melanogaster
Genetic Insert: Gle sgRNA Plasmid
Vector Backbone Description: Vector Backbone:pU6-BbsI-chiRNA; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35639929
Comments: sgRNA sequence: GCGTATCCCCTTAACAATAA. The corresponding genome sequence is: CCGTATCCCCTTAACAATAA.
Please visit https://www.biorxiv.org/content/10.1101/2020.12.18.423517v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_186658 Copy
Species: Drosophila melanogaster
Genetic Insert: Bcd
Vector Backbone Description: Vector Backbone:pBABR; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35320726
Proper citation: RRID:Addgene_182595 Copy
Species: Drosophila melanogaster
Genetic Insert: Rab4
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29937389
Comments: YFP Rab4 from Addgene 37690 was transferred to pcDNA3.1
Proper citation: RRID:Addgene_99018 Copy
Species: Drosophila melanogaster
Genetic Insert: Rab4 DN
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29937389
Comments: YFP Rab4DN from Addgene 37691 was transferred to pcDNA3.1
Proper citation: RRID:Addgene_99019 Copy
Species: Drosophila melanogaster
Genetic Insert: w+ gRNA and scaffold followed by 2 gRNA with BbsI and BsaI cloning sites
Vector Backbone Description: Backbone Marker:Perrimon lab modified in our lab; Backbone Size:5400; Vector Backbone:pCFD4; Vector Types:Insect Expression, CRISPR, Recombines at attp landing site in fly genome; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_183202 Copy
Species: Drosophila melanogaster
Genetic Insert: STX1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3538; Vector Backbone:pMT/V5-HIS; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_89310 Copy
Species: Drosophila melanogaster
Genetic Insert: HyperADARcd
Vector Backbone Description: Vector Backbone:pGX989; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34426573
Comments: Genbank: MW026670
Proper citation: RRID:Addgene_202579 Copy
Species: Drosophila melanogaster
Genetic Insert: HyperADARcd
Vector Backbone Description: Vector Backbone:pGX989; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34426573
Comments: Genbank: MW026671
Proper citation: RRID:Addgene_202580 Copy
Species: Drosophila melanogaster
Genetic Insert: HyperADARcd
Vector Backbone Description: Vector Backbone:pJG054; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34426573
Comments: Genbank: MW026680
Proper citation: RRID:Addgene_202584 Copy
Species: Drosophila melanogaster
Genetic Insert: Khd4-Ada-Gfp
Vector Backbone Description: Backbone Size:6763; Vector Backbone:pMF2-1c; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37590419
Proper citation: RRID:Addgene_203734 Copy
Species: Drosophila melanogaster
Genetic Insert: T2A-split-intein-Gal4[21-881]-C-int
Vector Backbone Description: Vector Backbone:Derived from pCRISPaint-T2A-Gal4-3xP3-RFP; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37276389
Proper citation: RRID:Addgene_199204 Copy
Species: Drosophila melanogaster
Genetic Insert: T2A-p65Zp
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:PCR Blunt II - TOPO; Vector Types:Insect Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37276389
Proper citation: RRID:Addgene_199200 Copy
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