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Species: Rattus norvegicus
Genetic Insert: CRY2PHR mCherry iTrkA
Vector Backbone Description: Backbone Size:3800; Vector Backbone:pmCherry N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29975841
Proper citation: RRID:Addgene_106168 Copy
Species: Rattus norvegicus
Genetic Insert: Lyn iTrkA mCherry CRY2PHR
Vector Backbone Description: Backbone Size:3800; Vector Backbone:pmCherry N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29975841
Proper citation: RRID:Addgene_106169 Copy
Species: Rattus norvegicus
Genetic Insert: TrkA mCherry CRY2
Vector Backbone Description: Backbone Size:3800; Vector Backbone:pmCherryN1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29975841
Proper citation: RRID:Addgene_106167 Copy
Species: Rattus norvegicus
Genetic Insert: GCaMP6f
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:8151; Vector Backbone:PLVX Puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31821412
Proper citation: RRID:Addgene_106298 Copy
Species: Rattus norvegicus
Genetic Insert: complexin-1 fragment 1-83
Vector Backbone Description: Vector Backbone:pGEX-6P-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28813412
Proper citation: RRID:Addgene_106417 Copy
Species: Rattus norvegicus
Genetic Insert: SNAP25 fragment 141-204
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pETDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28813412
Proper citation: RRID:Addgene_106415 Copy
Species: Rattus norvegicus
Genetic Insert: AE-Polyubiquitin-GCAaMP6s
Vector Backbone Description: Backbone Size:5246; Vector Backbone:PiggyBac; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31208328
Proper citation: RRID:Addgene_106868 Copy
Species: Rattus norvegicus
Genetic Insert: MBP-tagged rat pyruvate kinase M2 isoform
Vector Backbone Description: Backbone Size:4467; Vector Backbone:PCRT7; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31825824
Proper citation: RRID:Addgene_107158 Copy
Species: Rattus norvegicus
Genetic Insert: MBP-tagged rat pyruvate kinase liver isoform
Vector Backbone Description: Backbone Size:4467; Vector Backbone:PCRT7; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31825824
Proper citation: RRID:Addgene_107155 Copy
Species: Rattus norvegicus
Genetic Insert: CAMKII
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10777564
Comments: Description: Wild type CAMKII provided by H. Schulman (Vanderbilt) subcloned into the EcoRI site of CS2+. Linearize with Not 1 and transcribe with SP6.
Ref: M. Kuehl et al., JBC 275:12701-12711, 2000.
Proper citation: RRID:Addgene_16737 Copy
Species: Rattus norvegicus
Genetic Insert: CAMKII-K42M 1-271
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10777564
Comments: Description: A C-terminal truncation of K42M kinase dead CAMKII provided by H. Schulman (Vanderbilt), subcloned into the EcoRI site of CS2+. Linearize with Not 1 and transcribe with SP6.
Ref: M. Kuehl et al., JBC 275:12701-12711, 2000.
Proper citation: RRID:Addgene_16734 Copy
Species: Rattus norvegicus
Genetic Insert: mEGFP(A206K)-paCaMKII(I539E)-ShadowG
Vector Backbone Description: Vector Backbone:Customized Vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33531495
Comments: Only tested in HeLa cells, but not in neurons.
Proper citation: RRID:Addgene_167478 Copy
Species: Rattus norvegicus
Genetic Insert: mEGFP(A206K)-paCaMKII(C450A)-ShadowG
Vector Backbone Description: Vector Backbone:Customized Vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33531495
Comments: Only tested in HeLa cells, but not in neurons.
Proper citation: RRID:Addgene_167479 Copy
Species: Rattus norvegicus
Genetic Insert: LAMP1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4682; Vector Backbone:pmRFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28320970
Proper citation: RRID:Addgene_167970 Copy
Species: Rattus norvegicus
Genetic Insert: Rfz1
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Comments: Constructed by: Julia Yang-Snyder (subclone), Jeff Brown (tags), 4/19/96.
Rfz1 from Henk Roelink. This sequence has a few differences
from the published sequence. Sites listed in Rfz1 are not a complete list. MYC tag version made by Jeff Brown (2/98-3/98), inserted the epitope into the StyI site (1904). The MYC epitope has a BglII site in it. The 3'
Sty site is destroyed by the tags, but the 5' site is intact. Epitope can be detected in PBS/Formaldehyde fixed tissue permeabilized with 0.2% Triton.
The Myc tagged Rfz1 has been IP'd from a TBS/1% Triton buffer. Frizzled tends to ppt out of SDS sample buffer when boiled, so either warm samples
to ~70 degrees Celsius for a few minutes before loading samples or use a strong denaturing loading buffer and incubate the samples at room temp for half an hour before loading. The StyI site is in the 3rd extracellular loop of the protein.
Proper citation: RRID:Addgene_16798 Copy
Species: Rattus norvegicus
Genetic Insert: Rfz2 TG2myc
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Comments: Constructed by: Jeff Brown (tags), 11/23/98.
A single c-myc epitope has been inserted into the third extracellular loop of Rfz2 by introduction of an NheI site and the epitope. The epitope
was inserted in the region of the loop where the myc and the HA tags were inserted into the Rfz1 tagged constructs--3 aa's after the PCP sequence--which
appear to be functional.
*The ClaI and XbaI sites are methylated and cannot be cut unless the construct is grown up in dam-bugs.
Proper citation: RRID:Addgene_16796 Copy
Species: Rattus norvegicus
Genetic Insert: beta 2 AR Fz1
Vector Backbone Description: Backbone Size:5428; Vector Backbone:pCDNA3.1; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10588714
Comments: Chimera of beta 2 adrenergic receptor (extracellular and.
transmembrane domains) and rat frizzled-1 (intracellular). Activated by isoproterenol and inhibited by propranolol.
Ref: X. Lui et al., PNAS, 1999, 96:14383-14388 and M. Kuehl et al.,
2000, JBC 275:12701-12711.
Proper citation: RRID:Addgene_16795 Copy
Species: Rattus norvegicus
Genetic Insert: rat frizzled 2
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8939578
Comments: Insert size: ~1912 bp (EcoRI/Xba I both blunted)
Transcription: linearize with Asp 718 and transcribe with SP6.
Plasmid History:
Constructed by Julia Yang-Snyder on the date of 5/19/96.
Original reference: Chan, S.D.H., et al. JBC 267:25202-25207, 1992.
Published reference: Yang-Snyder, J.A. et al. Curr. Biol. 6:1302-1306,
1996.
Proper citation: RRID:Addgene_16814 Copy
Species: Rattus norvegicus
Genetic Insert: rat frizzled 1
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8939578
Comments: Transcription: linearize with Not I or Asp 718 and transcribe with SP6.
Plasmid History:
Constructed by Julia Yang-Snyder on the date of 4/29/96.
Original reference: Chan, S.D.H., et al. JBC 267:25202-25207, 1992.
Published reference: Yang-Snyder, J.A. et al. Curr. Biol. 6:1302-1306, 1996.
Proper citation: RRID:Addgene_16821 Copy
Species: Rattus norvegicus
Genetic Insert: frizzled-1
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pcS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Comments: The original rat Frizzled-1 cDNA was cloned into CS2+. The CMV promoter was removed as a Sal-I-HindIII fragment, and the SV40 promoter fragment of the vector pCD-PS (as a Sal-I-SmaI fragment) was cloned in, destroying the HindIII site. As a result, this construct places the cDNA under the control of an SV40 promoter.
Reference: The insert was published by Wang et al., JBC 271: 4468 (1996). This unpublished construct was generated by Julia Yang-Snyder.
Proper citation: RRID:Addgene_16820 Copy
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