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  • Organism:saccharomyces cerevisiae (facet)

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pFA6a-HISMX6-pSAE3
 
Resource Report
Resource Website 		RRID:Addgene_191791 HIS3 pSAE3 Saccharomyces cerevisiae Ampicillin Backbone Size:2448; Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin 2026-08-15 01:24:21 0
pFA6a-HISMX6-pSPO11
 
Resource Report
Resource Website 		RRID:Addgene_191790 HIS3 pSPO11 Saccharomyces cerevisiae Ampicillin Backbone Size:2448; Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin 2026-08-15 01:24:21 0
pFA6a-kanMX6-pIME2
 
Resource Report
Resource Website 		RRID:Addgene_191789 KanMX pIME2 Saccharomyces cerevisiae Ampicillin Backbone Size:2446; Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin 2026-08-15 01:24:21 0
pFA6a-kanMX6-pSAE3
 
Resource Report
Resource Website 		RRID:Addgene_191787 KanMX pSAE3 Saccharomyces cerevisiae Ampicillin Backbone Size:2446; Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin 2026-08-15 01:24:21 0
pTSK405
 
Resource Report
Resource Website 		RRID:Addgene_190817 URA3 Saccharomyces cerevisiae Ampicillin PMID:27566148 Backbone Size:7637; Vector Backbone:pTSK241; Vector Types:Bacterial cloning vector; Bacterial Resistance:Ampicillin 2026-08-15 01:24:24 0
Linked VLP NES (G)
 
Resource Report
Resource Website 		RRID:Addgene_182476 VP2C-FPPS(G)-NES Saccharomyces cerevisiae Ampicillin Please visit https://www.biorxiv.org/content/10.1101/2022.11.24.517869v1 for bioRxiv preprint. Vector Backbone:pILGFPB5A; Vector Types:Yeast Expression, Synthetic Biology, Metabolic engineering; Bacterial Resistance:Ampicillin 2026-08-15 01:24:28 0
Coexpressed VLP NES
 
Resource Report
Resource Website 		RRID:Addgene_182433 VP2C-FPPS Saccharomyces cerevisiae Ampicillin Please visit https://www.biorxiv.org/content/10.1101/2022.11.24.517869v1 for bioRxiv preprint. Vector Backbone:pILGFPB5A; Vector Types:Yeast Expression, Synthetic Biology, Metabolic engineering; Bacterial Resistance:Ampicillin 2026-08-15 01:24:28 0
Linked VLP NES
 
Resource Report
Resource Website 		RRID:Addgene_182432 VP2C-FPPS-NES Saccharomyces cerevisiae Ampicillin Please visit https://www.biorxiv.org/content/10.1101/2022.11.24.517869v1 for bioRxiv preprint. Vector Backbone:pILGFPB5A; Vector Types:Yeast Expression, Synthetic Biology, Metabolic engineering; Bacterial Resistance:Ampicillin 2026-08-15 01:24:28 0
Coexpressed VLP NES (M)
 
Resource Report
Resource Website 		RRID:Addgene_182435 VP2C-FPPS(M) Saccharomyces cerevisiae Ampicillin Please visit https://www.biorxiv.org/content/10.1101/2022.11.24.517869v1 for bioRxiv preprint. Vector Backbone:pILGFPB5A; Vector Types:Yeast Expression, Synthetic Biology, Metabolic engineering; Bacterial Resistance:Ampicillin 2026-08-15 01:24:28 0
Linked VLP NES (M)
 
Resource Report
Resource Website 		RRID:Addgene_182434 VP2C-FPPS(M)-NES Saccharomyces cerevisiae Ampicillin Please visit https://www.biorxiv.org/content/10.1101/2022.11.24.517869v1 for bioRxiv preprint. Vector Backbone:pILGFPB5A; Vector Types:Yeast Expression, Synthetic Biology, Metabolic engineering; Bacterial Resistance:Ampicillin 2026-08-15 01:24:28 0
pGAL-flp(H305L)-NAT
 
Resource Report
Resource Website 		RRID:Addgene_160380 FLP1 Saccharomyces cerevisiae Ampicillin NAT cassette amplified from pLND46 using primers GGT GAT GAC GGT GAA AAC CTC and CTG ATG CGG TAT TTT CTC CTT ACG . Vector flp(H305L)-URA3 (Yeast, Vol. 14: 847–852 (1998))amplified with oligos TGC GTA AGG AGA AAA TAC CGC & GTC AGA GGT TTT CAC CGT CAT outwards from the URA3 region to generate linear fragment with ends complementary to the NAT cassette. Both PCRs carried out using the Primestar MAX polymerase mix (Takara).The two products were then annealed using Gibson assembly, using 3X the amount of NAT PCR product compared to the flp PCR product. 2uL of the mix transformed into NEB 5-alpha competent cells, and transformants were miniprepped and checked for the correct marker replacement with BamH1-Nco1 restriction digest. New insert gives a smaller (1.2 kb) fragment than original vector (1.5kb fragment). Overexpression of flp(H305L) cures 2-micron plasmids. Backbone Marker:Yeast, Vol 14: 847–852 (1998); Backbone Size:6861; Vector Backbone:pBIS-GALkFLP(URA3); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin H305L 2026-08-15 01:24:29 0
pGAL-SKI1-NAT
 
Resource Report
Resource Website 		RRID:Addgene_160382 SKI1 Saccharomyces cerevisiae Ampicillin Backbone Marker:Genetics. 2009 Sep; 183(1): 365–383.; Backbone Size:5643; Vector Backbone:pLND46; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:24:29 0
LDB59
 
Resource Report
Resource Website 		RRID:Addgene_185436 NUP1 Saccharomyces cerevisiae Ampicillin PMID:7929578 Also used in Harper et al. (2008) "Mutations affecting spindle pole body and mitotic exit network function are synthetically lethal with a deletion of the nucleoporin NUP1 in S. cerevisiae." Current genetics. https://doi.org/10.1007/s00294-007-0168-4. PMID: 18058101 Vector Backbone:pRS316; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:23:22 0
KBB11
 
Resource Report
Resource Website 		RRID:Addgene_185422 KAP60 Saccharomyces cerevisiae Ampicillin PMID:8045927 Please note: KAP60/SRP1 is in the opposite orientation of other plasmids in the deposit making it downstream of the T3 promoter as opposed to downstream of T7 promoter. Vector Backbone:pRS314; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:23:23 0
LDB107
 
Resource Report
Resource Website 		RRID:Addgene_185423 NUP1 Saccharomyces cerevisiae Ampicillin PMID:8045927 Also used in Finn et al. (2013) "A subset of FG-nucleoporins is necessary for efficient Msn5-mediated nuclear protein export." Biochim Biophys Acta. doi.org/10.1016/j.bbamcr.2012.12.020. PMID: 23295456 Please note: HA-tag is in the middle of NUP1and the ORF in reverse orientation (downstream of T3 promoter) compared to other similar plasmids Vector Backbone:pRS314; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:23:22 0
KBB438
 
Resource Report
Resource Website 		RRID:Addgene_185092 RFA1 Saccharomyces cerevisiae Ampicillin PMID:21332387 Vector Backbone:pRS316; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin Rfa1 with amino acids 338-343 deleted 2026-08-15 01:23:23 0
KBB466
 
Resource Report
Resource Website 		RRID:Addgene_185099 NMA111 Saccharomyces cerevisiae Ampicillin PMID:19808885 Vector Backbone:pRS316; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin NMA111DNLS1DNLS2-GFP 2026-08-15 01:23:23 0
KBB280
 
Resource Report
Resource Website 		RRID:Addgene_185073 NMA111 Saccharomyces cerevisiae Ampicillin PMID:19808885 Vector Backbone:pRS316; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin NMA111 aa 1-83 2026-08-15 01:23:24 0
KBB282
 
Resource Report
Resource Website 		RRID:Addgene_185074 NMA111 Saccharomyces cerevisiae Ampicillin PMID:19808885 Vector Backbone:pRS316; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin NMA111 aa 1-35 2026-08-15 01:23:23 0
KBB258
 
Resource Report
Resource Website 		RRID:Addgene_185072 NUP1 Saccharomyces cerevisiae Ampicillin Vector Backbone:pGEX-2TK; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin Nup1 N-term+FXFG repeats 2026-08-15 01:23:23 0

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