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Species: Saccharomyces cerevisiae
Genetic Insert: HIS3 pSAE3
Vector Backbone Description: Backbone Size:2448; Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_191791 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HIS3 pSPO11
Vector Backbone Description: Backbone Size:2448; Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_191790 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: KanMX pIME2
Vector Backbone Description: Backbone Size:2446; Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_191789 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: KanMX pSAE3
Vector Backbone Description: Backbone Size:2446; Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_191787 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: URA3
Vector Backbone Description: Backbone Size:7637; Vector Backbone:pTSK241; Vector Types:Bacterial cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27566148
Proper citation: RRID:Addgene_190817 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: VP2C-FPPS(G)-NES
Vector Backbone Description: Vector Backbone:pILGFPB5A; Vector Types:Yeast Expression, Synthetic Biology, Metabolic engineering; Bacterial Resistance:Ampicillin
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.11.24.517869v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_182476 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: VP2C-FPPS
Vector Backbone Description: Vector Backbone:pILGFPB5A; Vector Types:Yeast Expression, Synthetic Biology, Metabolic engineering; Bacterial Resistance:Ampicillin
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.11.24.517869v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_182433 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: VP2C-FPPS-NES
Vector Backbone Description: Vector Backbone:pILGFPB5A; Vector Types:Yeast Expression, Synthetic Biology, Metabolic engineering; Bacterial Resistance:Ampicillin
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.11.24.517869v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_182432 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: VP2C-FPPS(M)
Vector Backbone Description: Vector Backbone:pILGFPB5A; Vector Types:Yeast Expression, Synthetic Biology, Metabolic engineering; Bacterial Resistance:Ampicillin
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.11.24.517869v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_182435 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: VP2C-FPPS(M)-NES
Vector Backbone Description: Vector Backbone:pILGFPB5A; Vector Types:Yeast Expression, Synthetic Biology, Metabolic engineering; Bacterial Resistance:Ampicillin
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.11.24.517869v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_182434 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: FLP1
Vector Backbone Description: Backbone Marker:Yeast, Vol 14: 847–852 (1998); Backbone Size:6861; Vector Backbone:pBIS-GALkFLP(URA3); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NAT cassette amplified from pLND46 using primers GGT GAT GAC GGT GAA AAC CTC and
CTG ATG CGG TAT TTT CTC CTT ACG . Vector flp(H305L)-URA3 (Yeast, Vol. 14: 847–852 (1998))amplified with oligos TGC GTA AGG AGA AAA TAC CGC & GTC AGA GGT TTT CAC CGT CAT outwards from the URA3 region to generate linear fragment with ends complementary to the NAT cassette. Both PCRs carried out using the Primestar MAX polymerase mix (Takara).The two products were then annealed using Gibson assembly, using 3X the amount of NAT PCR product compared to the flp PCR product. 2uL of the mix transformed into NEB 5-alpha competent cells, and transformants were miniprepped and checked for the correct marker replacement with BamH1-Nco1 restriction digest. New insert gives a smaller (1.2 kb) fragment than original vector (1.5kb fragment). Overexpression of flp(H305L) cures 2-micron plasmids.
Proper citation: RRID:Addgene_160380 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SKI1
Vector Backbone Description: Backbone Marker:Genetics. 2009 Sep; 183(1): 365–383.; Backbone Size:5643; Vector Backbone:pLND46; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_160382 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: NUP1
Vector Backbone Description: Vector Backbone:pRS316; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7929578
Comments: Also used in Harper et al. (2008) "Mutations affecting spindle pole body and mitotic exit network function are synthetically lethal with a deletion of the nucleoporin NUP1 in S. cerevisiae." Current genetics. https://doi.org/10.1007/s00294-007-0168-4. PMID: 18058101
Proper citation: RRID:Addgene_185436 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: KAP60
Vector Backbone Description: Vector Backbone:pRS314; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8045927
Comments: Please note: KAP60/SRP1 is in the opposite orientation of other plasmids in the deposit making it downstream of the T3 promoter as opposed to downstream of T7 promoter.
Proper citation: RRID:Addgene_185422 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: NUP1
Vector Backbone Description: Vector Backbone:pRS314; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8045927
Comments: Also used in Finn et al. (2013) "A subset of FG-nucleoporins is necessary for efficient Msn5-mediated nuclear protein export." Biochim Biophys Acta. doi.org/10.1016/j.bbamcr.2012.12.020. PMID: 23295456
Please note: HA-tag is in the middle of NUP1and the ORF in reverse orientation (downstream of T3 promoter) compared to other similar plasmids
Proper citation: RRID:Addgene_185423 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RFA1
Vector Backbone Description: Vector Backbone:pRS316; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21332387
Proper citation: RRID:Addgene_185092 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: NMA111
Vector Backbone Description: Vector Backbone:pRS316; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19808885
Proper citation: RRID:Addgene_185099 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: NMA111
Vector Backbone Description: Vector Backbone:pRS316; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19808885
Proper citation: RRID:Addgene_185073 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: NMA111
Vector Backbone Description: Vector Backbone:pRS316; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19808885
Proper citation: RRID:Addgene_185074 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: NUP1
Vector Backbone Description: Vector Backbone:pGEX-2TK; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_185072 Copy
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