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Species: Synthetic
Genetic Insert: Codon-optimised P38
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6633; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24043421
Proper citation: RRID:Addgene_47727 Copy
Species: Homo sapiens
Genetic Insert: CALM3
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4000; Vector Backbone:pGEMEX-2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9828009
Comments: We observed in preliminary experiments that IPTG added to the growth media did not induce substantial additional CLP expression. In addition, we found that the addition of glucose to the growth medium significantly inhibited CLP expression from this vector. An NdeI site at position 3259 has been abolished.
Proper citation: RRID:Addgene_47600 Copy
Species: Homo sapiens
Genetic Insert: CaM
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEYFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11278607
Proper citation: RRID:Addgene_47603 Copy
Species: Homo sapiens
Genetic Insert: CALML3
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_47604 Copy
Species: Synthetic
Genetic Insert: Codon-optimised P12
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6633; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24043421
Proper citation: RRID:Addgene_47725 Copy
Species: Homo sapiens
Genetic Insert: CaM
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_47602 Copy
Genetic Insert: TP-1 promoter
Vector Backbone Description: Backbone Marker:described in Kato, et al. 1997 PMID 9374409 ; Vector Backbone:pGA981-6; Vector Types:Notch reporter construct; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16508309
Comments: This plasmid was constructed from the pGa981-6 plasmid by replacing the luciferase gene in the original pGA981-6 plasmid with dsRedExpress (Clontech) using XhoI/SmaI sites.
The construction of pGA981-6 was described as: "Six copies of this oligonucleotide were cloned in the BamHI site:
5' strand GATCCCGACT CGTGGGAAAA TGGGCGGAAG GGCACCGTGG GAAAATAGTA............
3' strand ............GGCTGA GCACCCTTTT ACCCGCCTTC CCGTGGCACC CTTTTATCAT CTAG"
Please note that Addgene's sequencing results indicate that the first and fourth ERE-TP1 repeats differ from the above oligo sequence at the twelfth nucleotide (G->A). These differences may have occurred during construction of the original pGA981-6 plasmid.
Proper citation: RRID:Addgene_47683 Copy
Species: Homo sapiens
Genetic Insert: Parkin
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4698; Vector Backbone:pECFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19029340
Proper citation: RRID:Addgene_47560 Copy
Species: Synthetic
Genetic Insert: Codon-optimised MSP5
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6633; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24043421
Proper citation: RRID:Addgene_47718 Copy
Species: Entercoccus faecalis
Genetic Insert: ndoE
Vector Backbone Description: Backbone Marker:GE Helthcare; Backbone Size:4974; Vector Backbone:pGEX-5X-3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15028731
Proper citation: RRID:Addgene_47716 Copy
Species: Synthetic
Genetic Insert: promoter 14 and BCD20
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pFAB1781; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23474465
Comments: http://biofab.org/services
RFP fluorescence (Arbitrary Units, AU) is used to measure the efficiency of the promoter/BCD combination. Promoter/BCD combinations ranged from a low score of 6.14 to a high score of 634.54. The score for this combination was: 66.03
Proper citation: RRID:Addgene_47827 Copy
Species: Synthetic
Genetic Insert: promoter 14 and BCD21
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pFAB1781; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23474465
Comments: http://biofab.org/services
RFP fluorescence (Arbitrary Units, AU) is used to measure the efficiency of the promoter/BCD combination. Promoter/BCD combinations ranged from a low score of 6.14 to a high score of 634.54. The score for this combination was: 51.15
Proper citation: RRID:Addgene_47828 Copy
Species: Synthetic
Genetic Insert: promoter 14 and BCD22
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pFAB1781; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23474465
Comments: http://biofab.org/services
RFP fluorescence (Arbitrary Units, AU) is used to measure the efficiency of the promoter/BCD combination. Promoter/BCD combinations ranged from a low score of 6.14 to a high score of 634.54. The score for this combination was: 10.19
Proper citation: RRID:Addgene_47829 Copy
Species: Synthetic
Genetic Insert: Codon-optimised RAMA
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6633; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24043421
Proper citation: RRID:Addgene_47786 Copy
Species: Synthetic
Genetic Insert: Codon-optimised TLP
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6633; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24043421
Proper citation: RRID:Addgene_47787 Copy
Species: Synthetic
Genetic Insert: promoter 14 and BCD13
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pFAB1781; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23474465
Comments: http://biofab.org/services
RFP fluorescence (Arbitrary Units, AU) is used to measure the efficiency of the promoter/BCD combination. Promoter/BCD combinations ranged from a low score of 6.14 to a high score of 634.54. The score for this combination was: 96.71
Proper citation: RRID:Addgene_47820 Copy
Species: Synthetic
Genetic Insert: promoter 14 and BCD16
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pFAB1781; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23474465
Comments: http://biofab.org/services
RFP fluorescence (Arbitrary Units, AU) is used to measure the efficiency of the promoter/BCD combination. Promoter/BCD combinations ranged from a low score of 6.14 to a high score of 634.54. The score for this combination was: 40.4
Proper citation: RRID:Addgene_47823 Copy
Species: Synthetic
Genetic Insert: Codon-optimised PTEX150
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6633; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24043421
Proper citation: RRID:Addgene_47788 Copy
Species: Synthetic
Genetic Insert: promoter 14 and BCD15
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pFAB1781; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23474465
Comments: http://biofab.org/services
RFP fluorescence (Arbitrary Units, AU) is used to measure the efficiency of the promoter/BCD combination. Promoter/BCD combinations ranged from a low score of 6.14 to a high score of 634.54. The score for this combination was: 90.17
Proper citation: RRID:Addgene_47822 Copy
Species: Synthetic
Genetic Insert: Codon-optimised SPATR
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6633; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24043421
Proper citation: RRID:Addgene_47782 Copy
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