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Species: Mus musculus
Genetic Insert: c-src
Vector Backbone Description: Backbone Size:6400; Vector Backbone:pEVX; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7530829
Proper citation: RRID:Addgene_17685 Copy
Species: Mus musculus
Genetic Insert: c-src Y529F
Vector Backbone Description: Backbone Size:6400; Vector Backbone:pEVX; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7530829
Proper citation: RRID:Addgene_17686 Copy
Species: Mus musculus
Genetic Insert: VAMP2
Vector Backbone Description: Vector Backbone:EGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33658493
Proper citation: RRID:Addgene_166891 Copy
Species: Mus musculus
Genetic Insert: VAMP2
Vector Backbone Description: Vector Backbone:EGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33658493
Proper citation: RRID:Addgene_166890 Copy
Species: Mus musculus
Genetic Insert: KIF5A-R280H
Vector Backbone Description: Vector Backbone:pLV-eF1a-IRES::Puro; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33284322
Proper citation: RRID:Addgene_166953 Copy
Species: Mus musculus
Genetic Insert: KIF5A-R280H
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33284322
Proper citation: RRID:Addgene_166957 Copy
Species: Mus musculus
Genetic Insert: KIF5A
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33284322
Proper citation: RRID:Addgene_166956 Copy
Species: Mus musculus
Genetic Insert: KIF5A
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33284322
Proper citation: RRID:Addgene_166955 Copy
Species: Mus musculus
Genetic Insert: KLC1
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33284322
Proper citation: RRID:Addgene_166962 Copy
Species: Mus musculus
Genetic Insert: sFRP-1
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_16693 Copy
Species: Mus musculus
Genetic Insert: Mouse Naked-2 pCS2P+F
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2P+P; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15539487
Comments: Description: Subcloned Keith Wharton pCDNA3 insert into Xba (blunted) and Xho sites of CS2P+
Linearize with Sac2 and transcribe SP6.
Reference: Waxman JS, Hocking AM, Stoick CL, Moon RT.
Zebrafish Dapper1 and Dapper2 play distinct roles in Wnt-mediated developmental processes.
Development. 2004 Dec;131(23):5909-21.
* Addgene's QC finds a 163bp deletion at the 3'end when compared to the NCBI reference sequence
Proper citation: RRID:Addgene_16727 Copy
Species: Mus musculus
Genetic Insert: dnPKA
Vector Backbone Description: Backbone Size:4100; Vector Backbone:CS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8812120
Comments: Insert: dominant negative Protein Kinase A-bright green flourescent protein fusion protein.
To make sense RNA, for expression, cut with Not1 and transcribe with SP6.
Plasmid History:
Constructed by Anne Ungar in 1996.
Citation: Ungar and Moon (1996)
Developmental Biology 178:186-191.
Proper citation: RRID:Addgene_16716 Copy
Species: Mus musculus
Genetic Insert: Casein Kinase II beta-pCS2P+
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2P+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15539487
Comments: Description: Subcloned from XE 164 into Stu/Xba sites of CS2P+
Linearize with ASP718 and transcribe SP6.
Reference: Waxman JS, Hocking AM, Stoick CL, Moon RT.
Zebrafish Dapper1 and Dapper2 play distinct roles in Wnt-mediated developmental processes.
Development. 2004 Dec;131(23):5909-21.
Proper citation: RRID:Addgene_16729 Copy
Species: Mus musculus
Genetic Insert: Mouse Naked-1
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2P+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15539487
Comments: Description: Subcloned Keith Wharton pCDNA3 insert into Xba (blunted) and Xho sites of CS2P+
Linearize with Sac2 and transcribe SP6.
Reference: Waxman JS, Hocking AM, Stoick CL, Moon RT.
Zebrafish Dapper1 and Dapper2 play distinct roles in Wnt-mediated developmental processes.
Development. 2004 Dec;131(23):5909-21.
Addgene QC found an E431K mutation that is not known to affect function.
Proper citation: RRID:Addgene_16728 Copy
Species: Mus musculus
Genetic Insert: Ribo-GCaMP8m
Vector Backbone Description: Backbone Size:2868; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36739289
Proper citation: RRID:Addgene_167574 Copy
Species: Mus musculus
Genetic Insert: gRNA targeting Zmat3
Vector Backbone Description: Vector Backbone:pLL3.3; Vector Types:Mammalian Expression, Mouse Targeting, Lentiviral, Cre/Lox, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33157015
Proper citation: RRID:Addgene_167855 Copy
Species: Mus musculus
Genetic Insert: sgRNA targeting Zmat3
Vector Backbone Description: Vector Backbone:pLL3.3; Vector Types:Mammalian Expression, Mouse Targeting, Lentiviral, Cre/Lox, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33157015
Proper citation: RRID:Addgene_167853 Copy
Species: Mus musculus
Genetic Insert: mFZ6-CS2+
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10395542
Comments: Insert: The clone mFZ4/pRK5 from Nathans lab (accession # U43319), was used as template to PCR amplify just the coding region as an EcoR1 insert. The 3' stop was kept.
Constructed by: Rebecca Bates/Randall Moon.
Notes for Use:
Ref: Sheldahl et al., Current Biology, 9:695-698, 1999.
Proper citation: RRID:Addgene_16802 Copy
Species: Mus musculus
Genetic Insert: FZ7
Vector Backbone Description: Backbone Size:4095; Vector Backbone:PCS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10395542
Comments: Insert: 3 Kb 5' EcoRI - 3' XbaI insert of mFZ7/pRK5 (Nathans), with engineered optimal kozak sequence.
Plasmid History:
Species of Sequence Origin: Xenopus.
Constructed by: Rebecca Bates/Randall Moon.
Ref: Sheldahl et al., Current Biology, 9:695-698, 1999.
Proper citation: RRID:Addgene_16801 Copy
Species: Mus musculus
Genetic Insert: Hrasls3
Vector Backbone Description: Backbone Marker:Dr.Shoji Yamaoka of Tokyo Medical and Dental University; Backbone Size:6404; Vector Backbone:pMRX-IPU-EGFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33854238
Proper citation: RRID:Addgene_167987 Copy
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