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  • Organism:rattus norvegicus (facet)


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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pCAG mEmerald-LAMP1
 
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RRID:Addgene_168513 LAMP1 Rattus norvegicus Ampicillin PMID:23791179 Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:09:55 0
pCS2-LAMP1-EGFP
 
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RRID:Addgene_169003 rat LAMP1-EGFP Rattus norvegicus Ampicillin PMID:33296658 Backbone Size:4125; Vector Backbone:pCS2; Vector Types:Unspecified; Bacterial Resistance:Ampicillin 2026-08-15 01:09:56 0
pLIC-His-GBAi
 
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RRID:Addgene_171755 GBAi Rattus norvegicus Ampicillin PMID:29133411 Backbone Size:6000; Vector Backbone:pLIC-His; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:10:19 0
pLIC-myc-GBAi
 
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RRID:Addgene_171753 GBAi Rattus norvegicus Ampicillin PMID:29133411 Backbone Size:6000; Vector Backbone:pLIC-myc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:10:19 0
pAd-PV-NLS-DsRed
 
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1+ mentions
RRID:Addgene_17287 PV-DsRed-NLS-CD Rattus norvegicus Kanamycin PMID:17420246 AdEasy is a registered trademark of the Johns Hopkins University. Backbone Size:33400; Vector Backbone:Adenoviral Recombinant (pAdEasy); Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin 2026-08-15 01:10:28 1
pCMV-PV-NLS-GFP
 
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1+ mentions
RRID:Addgene_17300 PV-GFP-NLS Rattus norvegicus Ampicillin PMID:11971908 Backbone Size:5900; Vector Backbone:pCMV-Myc-Cyto-GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:10:29 2
DHFR-GFP-th-GluA1
 
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RRID:Addgene_173008 DHFR-GFP-th-GluA1 Rattus norvegicus Ampicillin PMID:34241635 Please visit https://www.biorxiv.org/content/10.1101/2020.09.02.278770v1 for bioRxiv preprint. Backbone Size:8420; Vector Backbone:pLenti; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin 2026-08-15 01:10:29 0
pAAV-GFAP-jGCaMP7c variant 1513
 
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RRID:Addgene_173159 jGCaMP7c variant 1513-WPRE Rattus norvegicus Ampicillin Backbone Size:4764; Vector Backbone:pAAV-GFAP-EGFP; Vector Types:AAV; Bacterial Resistance:Ampicillin 2026-08-15 01:10:31 0
prGT3
 
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RRID:Addgene_15993 GLUT1 Rattus norvegicus Ampicillin PMID:3016720 Backbone Marker:Stratagene; Backbone Size:3200; Vector Backbone:pBS+; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:08:36 0
pAMPK alpha2 WT
 
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10+ mentions
RRID:Addgene_15991 AMPK alpha2 Rattus norvegicus Ampicillin PMID:11389854 Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:08:35 15
AllCB shRNA-1
 
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RRID:Addgene_160065 ARHGEF9 Rattus norvegicus Ampicillin PMID:33316079 Targets all collybistin mRNAs (target is located in the protein coding region. Nucleotides 1316-1335 of AJ302676 [CB2SH3-] or 1496-1515 of AJ250425 [CB1SH3+]. Targets all collybistin isoforms). Vector Backbone:mu6pro; Vector Types:RNAi; Bacterial Resistance:Ampicillin 2026-08-15 01:08:35 0
CBSH3+ 3m shRNA-1
 
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RRID:Addgene_160067 ARHGEF9 Rattus norvegicus Ampicillin PMID:33316079 3-point mutation negative control for CBSH3+ shRNA-1 targeting collybistin isoforms with SH3+ domain Vector Backbone:mu6pro; Vector Types:RNAi; Bacterial Resistance:Ampicillin GGcTGaTTaCAACAAGGATTG (mutations shown in lower case) 2026-08-15 01:08:38 0
CBSH3+ shRNA-2
 
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RRID:Addgene_160068 ARHGEF9 Rattus norvegicus Ampicillin PMID:33316079 Targets collybistin SH3+ mRNAs (target is located in the protein coding region of SH3+. Nucleotides 498-518 of AJ250425 [CB1SH3+]. Targets collybistin isoforms with SH3 domain). Vector Backbone:mu6pro; Vector Types:RNAi; Bacterial Resistance:Ampicillin 2026-08-15 01:08:35 0
pAAV-hSyn-DIO-HA-CB2SH3(-)-IRES-mCitrine
 
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RRID:Addgene_160069 ARHGEF9 Rattus norvegicus Ampicillin PMID:34083383 Backbone Marker:Modified from Bryan Roth (Addgene plasmid # 65417); Vector Backbone:pAAV2; Vector Types:AAV; Bacterial Resistance:Ampicillin 2026-08-15 01:08:35 0
CBSH3- 3m shRNA3
 
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RRID:Addgene_160213 ARHGEF9 Rattus norvegicus Ampicillin PMID:33316079 3-point mutation negative control for CBSH3- shRNA3 targeting collybistin MYD-CBSH3- isoforms (Addgene Plasmid #160212). Vector Backbone:mu6pro; Vector Types:RNAi; Bacterial Resistance:Ampicillin TGTATGACCTCaGGcTGtACCA (mutations shown in lower case) 2026-08-15 01:08:39 0
pSAC5.02
 
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1+ mentions
RRID:Addgene_16077 soluble adenylyl cyclase Rattus norvegicus Kanamycin PMID:9874775 Original library clone from Lambda-Zap rat testis library. CTCT repeats inserted between EcoRI sites in 5' UTR. Clone starts at nucleotide 241 of sAC sequence. EcoRI/XhoI digest: 0.7, 1.1, 1.5, 2.0, 4.5 kb. pSAC5.02 is derived from an original library clone obtained in the cloning of rat sAC. The isolation of this cDNA clone seems outdated now, but it was still a current method back in 1999, when we cloned sAC. We obtained peptide sequences from purified sAC protein, and designed PCR primers to those peptides. These resulted in isolation of a 1 kb fragment encoding a portion of the sAC cDNA, but there was open reading frame on each end of this PCRd clone. We then screened a rat testis library we generated in the lamdba zap cloning system. We screened the library by hybridization using the 1 kb PCR fragment as probe and we identified three, independent cDNA clones. THe lambda zap cloning system that we used enabled isolation of plasmids from the lambda phage library using helper phage. THe resultant plasmids contained library inserts (in our case, a sAC cDNA) in the pBK-CMV cloning vector. In this cloning vector, the cDNAs were inserted directionally, meaning that the vectors were already suitable for bacterial expression using the T7 promoter. We were unable to get sufficient protein via bacterial expression, so we deleted the T7 promoter region by digesting with NheI-SpeI and reclosing the vector; this brought the CMV promoter closer to the inserted sAC cDNA and facilitated high level expression in mammalian cells (i.e., HEK293 cells). This is how plasmid psAC5.02 was generated. THe CTCT repeats are within the 5' UTR of the sAC cDNA - they may have been a cloning artifact during generation of the library, or they may represent some 5' control element. The cDNA clone can not be moved (in its entirety) as an EcoR1 fragment (the pBK-CMV vector has EcoRI sites flanking the inserted cDNA) because there is an internal EcoR1 site within the sAC cDNA. EcoR1 can be used as a diagnostic digest to confirm the validity of the plasmid. Backbone Marker:Stratagene; Backbone Size:4500; Vector Backbone:pBK-CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:08:43 1
PKC beta I DN
 
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1+ mentions
RRID:Addgene_16381 PKC beta 1 Rattus norvegicus Ampicillin PMID:12794082 From user-provided sequence (see 'Reviews'), there are five amino acid differences from one of two NCBI reference sequences (AAA41868.1 and NP_001165776.1): 25A, 62F, 64K, 69C, and 294G. These "mutations" are also present in human, bovine, and xenopus sequences and are therefore not believed to affect function. Backbone Size:5400; Vector Backbone:pHACE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Dominant negative (kinase inactive): K371R 2026-08-15 01:09:15 3
CMV-LAMP1-HaloTag
 
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1+ mentions
RRID:Addgene_164209 LAMP1 Rattus norvegicus Kanamycin PMID:31539493 Vector Backbone:N/A; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:09:19 1
pETM10 with rat FL Annexin A11
 
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RRID:Addgene_164496 rat FL Annexin A11 Rattus norvegicus Kanamycin PMID:32344647 Backbone Marker:Gunter Stier; Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:09:21 0
pLVX-IRES-Hygro_GCaMP6s
 
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1+ mentions
RRID:Addgene_164591 GCaMP6s Rattus norvegicus Ampicillin PMID:31346185 Backbone Marker:Clontech; Backbone Size:8513; Vector Backbone:pLVX-IRES-Hygro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin 2026-08-15 01:09:22 1

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