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Genetic Insert: MCP-YFP
Vector Backbone Description: Vector Backbone:pLenti; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28290446
Proper citation: RRID:Addgene_101160 Copy
Genetic Insert: YPet(short)-HP35st-mCherry
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:6238; Vector Backbone:pLPCX; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26523364
Proper citation: RRID:Addgene_101251 Copy
Species: Homo sapiens
Genetic Insert: kappa-HA-dL5-myc-ADRB2
Vector Backbone Description: Backbone Size:6437; Vector Backbone:pBABE-SacLac; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29283245
Comments: Originally used in publication:
John Holleran, Dara Brown,* Margaret H. Fuhrman, Sally A. Adler, Gregory W. Fisher, and Jonathan W. Jarvik
Fluorogen-Activating Proteins as Biosensors of Cell-Surface Proteins in Living Cells
Cytometry A. 2010 Aug; 77(8): 776–782.
Proper citation: RRID:Addgene_101253 Copy
Genetic Insert: YPet(short)-HP35-mCherry
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:6238; Vector Backbone:pLPCX; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26523364
Proper citation: RRID:Addgene_101250 Copy
Vector Backbone Description: Backbone Size:5802; Vector Backbone:pSNAP-tag (m) Vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Codon usage of the gene is optimized for expression in mammalian cells. pSNAP-tag(m) contains two multiple cloning sites to allow cloning of the fusion partner as a fusion to the N- or C-terminus of the SNAP-tag. to allow cloning of the fusion partner as a fusion to the N- or C-terminus of the SNAP-tag. For more information on this plasmid, please see https://www.addgene.org/depositor-collections/neb-cell-imaging-tags/
This plasmid is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].
Proper citation: RRID:Addgene_101135 Copy
Vector Backbone Description: Backbone Size:3725; Vector Backbone:pSNAP-tag (T7) Vector; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: pSNAP-tag(T7) is a high copy number E. coli expression plasmid encoding SNAP26b, a SNAP-tag protein, which can be expressed under the control of the T7 promoter. Codon usage of the gene is optimized for expression in E. coli. The fusion partner should be cloned as a fusion to the C-terminus of the SNAP-tag. For more information on this plasmid, please see https://www.addgene.org/depositor-collections/neb-cell-imaging-tags/
This plasmid is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].
Proper citation: RRID:Addgene_101137 Copy
Genetic Insert: ffDronpa-F
Vector Backbone Description: Backbone Size:2860; Vector Backbone:pRSETb; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28561832
Proper citation: RRID:Addgene_101193 Copy
Species: Synthetic
Genetic Insert: LOV2 and STIM1 cytosolic fragment
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pTriEx; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26646180
Proper citation: RRID:Addgene_101245 Copy
Species: Mus musculus
Genetic Insert: Rock2
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26368311
Proper citation: RRID:Addgene_101296 Copy
Species: Mus musculus
Genetic Insert: alpha-Catenin
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28329679
Comments: The TS module with mTFP1-F40-venusA206K was adapted from Vinculin TS (a kind gift from Martin Schwartz [Grashoff et al., 2010]). The TSmod was cloned in to pEGFP-N1 vector at Xho1 and Not1 restriction sites. For aE-Cat-TS, the mouse N-terminal (1_697 aa) of alpha-catenin was inserted before mTFP1 by in- fusion cloning (Takara Bioscience, Clontech) at the Xho1 restriction site
Proper citation: RRID:Addgene_101298 Copy
Species: Mus musculus
Genetic Insert: alpha-Catenin
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28329679
Comments: The C-terminal fragment (698-906 aa) of alpha-catenin was cloned after VenusA206K at the Not1 restriction site
Proper citation: RRID:Addgene_101299 Copy
Genetic Insert: NL4-3 envFS
Vector Backbone Description: Vector Backbone:pBluescript; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30546110
Proper citation: RRID:Addgene_101333 Copy
Genetic Insert: NL4-3 envFS
Vector Backbone Description: Vector Backbone:pBluescript; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30546110
Proper citation: RRID:Addgene_101334 Copy
Genetic Insert: NL4-3
Vector Backbone Description: Vector Backbone:pNL4-4; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30546110
Comments: The last methionine in Gag (M423) was left as it was outside the ApaI site used to clone in the mutations. However, C-terminus of Gag is functionally inert, and Pol slips 27 nucleotides away and is unlikely to produce a stable protein product.
Proper citation: RRID:Addgene_101342 Copy
Species: Synthetic
Genetic Insert: ASAP2s
Vector Backbone Description: Backbone Size:4562; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28749338
Comments: The next-generation JEDI-2P sensors are available at: https://www.addgene.org/browse/article/28223314/
Proper citation: RRID:Addgene_101276 Copy
Species: Mesoplasma florum
Genetic Insert: oriC2 of M. florum strain L1 (dnaA/dnaN intergenic region)
Vector Backbone Description: Vector Backbone:colE1; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:28115382
Proper citation: RRID:Addgene_101310 Copy
Species: Mus musculus
Genetic Insert: E-cadherin
Vector Backbone Description: Backbone Marker:J. Bear (UNC Chapel Hill, USA); Vector Backbone:LentiLox pLL5.0(backbone pLL3.7); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24413434
Proper citation: RRID:Addgene_101278 Copy
Species: Mesoplasma florum
Genetic Insert: oriC3 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, w/o dnaA)
Vector Backbone Description: Vector Backbone:colE1; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:28115382
Proper citation: RRID:Addgene_101311 Copy
Species: Homo sapiens
Genetic Insert: Utrophin
Vector Backbone Description: Vector Backbone:pCS2.0; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24413434
Proper citation: RRID:Addgene_101279 Copy
Genetic Insert: NL4-3 vpr
Vector Backbone Description: Vector Backbone:pALPS; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30546110
Proper citation: RRID:Addgene_101329 Copy
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