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Vector Backbone Description: Vector Backbone:pAPIC; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:28874572
Comments: synthetic LexAGAD size is 2850 bp
For more information about the Han Lab Drosophila Transgenic Vectors, please visit: https://han.wicmb.cornell.edu/han-lab-drosophila-transgenic-vectors/.
Proper citation: RRID:Addgene_112807 Copy
Vector Backbone Description: Backbone Marker:Edouard Evangelisti, Liron Shenhav; Backbone Size:8431; Vector Backbone:pTORGm34GWF; Vector Types:Oomycete expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:31775609
Proper citation: RRID:Addgene_112904 Copy
Vector Backbone Description: Backbone Marker:Edouard Evangelisti, Liron Shenhav; Backbone Size:8701; Vector Backbone:pTORKm34GWY; Vector Types:Oomycete expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:31575765
Comments: Please visit https://www.biorxiv.org/content/10.1101/652255v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_112901 Copy
Vector Backbone Description: Backbone Marker:Edouard Evangelisti, Liron Shenhav; Backbone Size:8692; Vector Backbone:pTORKm34GWC; Vector Types:Oomycete expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:31575765
Comments: Please visit https://www.biorxiv.org/content/10.1101/652255v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_112898 Copy
Vector Backbone Description: Backbone Marker:Edouard Evangelisti, Liron Shenhav; Backbone Size:6856; Vector Backbone:pTORKm34GW; Vector Types:Oomycete expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:31575765
Comments: Please visit https://www.biorxiv.org/content/10.1101/652255v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_112897 Copy
Vector Backbone Description: Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression, Gateway shuttle vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:29997244
Proper citation: RRID:Addgene_113448 Copy
Vector Backbone Description: Backbone Marker:FUGW was a gift from David Baltimore (Addgene plasmid # 14883); Backbone Size:9955; Vector Backbone:FUGW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:29997244
Proper citation: RRID:Addgene_113456 Copy
Vector Backbone Description: Backbone Marker:FUGW was a gift from David Baltimore (Addgene plasmid # 14883); Backbone Size:9955; Vector Backbone:FUGW; Vector Types:Mammalian Expression, Lentiviral, Luciferase; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:29997244
Proper citation: RRID:Addgene_113455 Copy
Vector Backbone Description: Backbone Marker:FUGW was a gift from David Baltimore (Addgene plasmid # 14883); Backbone Size:9955; Vector Backbone:FUGW; Vector Types:Mammalian Expression, Lentiviral, Luciferase; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:29997244
Proper citation: RRID:Addgene_113454 Copy
Vector Backbone Description: Backbone Marker:Genetic Perturbation Platform; Backbone Size:9879; Vector Backbone:pLX_TRC311; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_113668 Copy
Species: Other
Genetic Insert: gateway cassette
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3048; Vector Backbone:pGEM-T easy; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:31523744
Comments: Use this vector for 3-way multi-site Gateway reactions with two regions of homology flanking an insert - this final vector can be used as a template for repair (homology-directed repair) after CRISPR double-strand break. This plasmid was constructed by A-tailing the Gateway cassette (R1R2) and blunt/TA Ligase reaction with pGEM-T Easy to create pGEM-gate.
Proper citation: RRID:Addgene_113758 Copy
Species: S. pyogenes, Z. mays
Genetic Insert: Cas9
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGEM-T easy; Vector Types:CRISPR, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:31523744
Comments: Also has aminoglycosyl phosphotransferase driven by 35S promoter (G418 resistance). We are not sure of the backbone -- it came from a plasmid that was made many many years ago. It aligns well with pGEM-T Easy (which a lot of our plasmids are based on) so if we had to guess, we would say the backbone is pGEM-T Easy.
Proper citation: RRID:Addgene_113743 Copy
Species: S. pyogenes, Z. mays
Genetic Insert: Cas9
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGEM-T easy; Vector Types:CRISPR, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:31523744
Comments: Also has hygromycinR driven by 35S promoter. We are not sure of the backbone -- it came from a plasmid that was made many many years ago. It aligns well with pGEM-T Easy (which a lot of our plasmids are based on) so if we had to guess, we would say the backbone is pGEM-T Easy.
Proper citation: RRID:Addgene_113742 Copy
Species: S. pyogenes, Z. mays
Genetic Insert: Cas9
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGEM-T easy; Vector Types:CRISPR, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:31523744
Comments: Also has zeocinR driven by 35S promoter. We are not sure of the backbone -- it came from a plasmid that was made many many years ago. It aligns well with pGEM-T Easy (which a lot of our plasmids are based on) so if we had to guess, we would say the backbone is pGEM-T Easy.
Proper citation: RRID:Addgene_113744 Copy
Vector Backbone Description: Vector Backbone:pFRT-EGFP-DEST; Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:28297718
Proper citation: RRID:Addgene_106348 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: 4 x upstream activating sequence (UAS)
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3078; Vector Backbone:Unknown; Vector Types:STAP-seq screening vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:31092928
Proper citation: RRID:Addgene_125149 Copy
Vector Backbone Description: Backbone Marker:our group built; Backbone Size:10091; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression, Bacterial Expression, in vitro expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:31467278
Comments: Please visit https://www.biorxiv.org/content/10.1101/530790v1.full for bioRxiv preprint.
Proper citation: RRID:Addgene_125547 Copy
Vector Backbone Description: Backbone Marker:we built; Backbone Size:8992; Vector Backbone:custom made; Vector Types:Mammalian Expression, Yeast Expression, in vitro expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:31467278
Comments: Please visit https://www.biorxiv.org/content/10.1101/530790v1.full for bioRxiv preprint.
Proper citation: RRID:Addgene_125548 Copy
Vector Backbone Description: Backbone Marker:we built; Backbone Size:8993; Vector Backbone:custom made; Vector Types:Mammalian Expression, Yeast Expression, in vitro expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:31467278
Comments: Please visit https://www.biorxiv.org/content/10.1101/530790v1.full for bioRxiv preprint.
Proper citation: RRID:Addgene_125559 Copy
Species: E.coli
Genetic Insert: ccdB Cassette (BsaI)
Vector Backbone Description: Vector Backbone:pICH47742; Vector Types:Plant Expression, Binary, Module 1; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:31085714
Proper citation: RRID:Addgene_126077 Copy
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