Searching the RRID Resource Information Network
Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
Vector Backbone Description: Vector Backbone:pK18; Vector Types:Bacterial Expression, Cre/Lox, Herpesvirus Cloning; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:10888635
Comments: The BAC vector is derived from the E.coli F plasmid. The BAC vector was cloned into the high copy vector pK18, to increase yields. The BAC vector is typically excised by PacI. The BAC vector contains genes for participation (sopA, B,C, a repE gene (required for replication) and an origin of replication (oriS). There is also a chloramphenicol resistance gene. The BAC vector carries a gpt gene, driven by the HSV tk promoter; the gpt gene is followed by the SV40 polyA signal sequence (ca 125 bp). The BAC vector is terminally flanked by loxP sites. The plasmid contains also a 1.5 kb EcoRI-fragment from the murine herpesvirus MHV68. The sequence of pK18 is available from Genbank ACCESSION M17626.
Proper citation: RRID:Addgene_85709 Copy
Vector Backbone Description: Backbone Marker:Invitrogen ; Backbone Size:4762; Vector Backbone:pDONR 221; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:28017692
Comments: Please note that there are some seqeunce discrepancies between Addgene's quality control sequences and the depositor's genbank file. The depositor noted that these discrepancies do NOT affect plasmid function.
Proper citation: RRID:Addgene_85752 Copy
Vector Backbone Description: Vector Backbone:pMDC32; Vector Types:Plant Expression, Gateway compatible attR1-attR2 destination vector; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:28211909
Comments: Note: Addgene's quality control sequencing finds a 1.2kb transposable element upstream of the Kanamycin resistance cassette. This inserted sequence does not affect plasmid function.
Proper citation: RRID:Addgene_86207 Copy
Vector Backbone Description: Backbone Marker:N/A; Backbone Size:4559; Vector Backbone:pSAM_Ec; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:26060277
Comments: Mariner himar1C9 transposase driven by lac promoter on an R6K gamma suicide plasmid. The transposon allows identification of fitness factors by Illumina sequencing. This transposon will randomly insert a kanamycin resistance cassette into genomic TA sites. Chloramphenicol resistance is contained on the plasmid backbone.
For further background, see:
Goodman, Andrew L., Meng Wu, and Jeffrey I. Gordon. “Identifying Microbial Fitness Determinants by Insertion Sequencing (INSeq) Using Genome-Wide Transposon Mutant Libraries.” Nature Protocols 6.12 (2011): 1969–1980. PMC. Web. 11 Apr. 2017.
Wiles, Travis J. et al. “Combining Quantitative Genetic Footprinting and Trait Enrichment Analysis to Identify Fitness Determinants of a Bacterial Pathogen.” Ed. Danielle A. Garsin. PLoS Genetics 9.8 (2013): e1003716. PMC. Web. 11 Apr. 2017.
Proper citation: RRID:Addgene_91570 Copy
Vector Backbone Description: Backbone Marker:PMID: 14736459; Backbone Size:4774; Vector Backbone:PDONR-221 P1-P2; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:17878951
Comments: Backbone was modified with ApaI (attP3) / EcoRI (attP5) and PstI (attP3) / EcoRV (attP5) sites
Proper citation: RRID:Addgene_186350 Copy
Vector Backbone Description: Backbone Marker:PMID: 14736459; Backbone Size:4768; Vector Backbone:PDONR-221 P1-P2; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:17878951
Comments: Backbone was modified with ApaI (attP3) / EcoRI (attP4) and PstI (attP3) / EcoRV (attP4) sites
Proper citation: RRID:Addgene_186351 Copy
Vector Backbone Description: Backbone Marker:6207; Vector Backbone:pUC-like; Vector Types:Unspecified; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:35927480
Comments: Some parts of the plasmid were synthesized by GeneArt (Thermo Fisher)
Proper citation: RRID:Addgene_165146 Copy
Vector Backbone Description: Vector Backbone:pENTR/D derivative; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:29847550
Proper citation: RRID:Addgene_105337 Copy
Vector Backbone Description: Vector Backbone:pJOG397 (pENTR/D derivative); Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:29847550
Comments: Please note the orientation of the elements inside the Gateway cassettes are flipped. The depositing lab has noted that this orientation will not affect plasmid function.
Proper citation: RRID:Addgene_105340 Copy
Vector Backbone Description: Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Proper citation: RRID:Addgene_124245 Copy
Vector Backbone Description: Vector Backbone:pSC218; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:29087011
Comments: 112-31:9.1. Alternative name of plasmid: pSG218RG.
Proper citation: RRID:Addgene_104780 Copy
Species: Synthetic
Genetic Insert: Lvl1 P2 With 3'UTR
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:30327560
Proper citation: RRID:Addgene_115956 Copy
Species: Synthetic
Genetic Insert: Lvl1 P2 No 3'UTR
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:30327560
Proper citation: RRID:Addgene_115957 Copy
Species: Synthetic
Genetic Insert: Lvl1 P1 With 3'UTR
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:30327560
Proper citation: RRID:Addgene_115953 Copy
Species: Spinacia oleracea
Genetic Insert: chloroplastic FNR transit peptide
Vector Backbone Description: Vector Backbone:pAGM4723; Vector Types:Plant Expression, Binary; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:31085714
Comments: Please incubate bacteria at 30 degrees Celsius after transformations to increase efficiency. Further growth after transformants are obtained should be performed at 37 degrees Celsius.
Proper citation: RRID:Addgene_126054 Copy
Species: Spinacia oleracea
Genetic Insert: chloroplastic FNR transit peptide
Vector Backbone Description: Vector Backbone:pAGM4723; Vector Types:Plant Expression, Binary; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:31085714
Comments: Please incubate bacteria at 30 degrees Celsius after transformations to increase efficiency. Further growth after transformants are obtained should be performed at 37 degrees Celsius.
Proper citation: RRID:Addgene_126055 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtTAS1c-D2-B/c
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Backbone Size:2580; Vector Backbone:pENTR; Vector Types:GATEWAY-compatible entry clone; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:32396204
Proper citation: RRID:Addgene_137883 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtTAS1c-D2-B/c
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Backbone Size:10141; Vector Backbone:pMDC32; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:32396204
Proper citation: RRID:Addgene_137884 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtTAS1c-D2-B/c
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Backbone Size:10141; Vector Backbone:pMDC32; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:32396204
Proper citation: RRID:Addgene_137885 Copy
Species: Synthetic
Genetic Insert: Type 4 Kanamycin resistance cassette
Vector Backbone Description: Backbone Size:1927; Vector Backbone:pBTK1001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:37786689
Comments: Please visit https://doi.org/10.1101/2023.09.19.558440 for bioRxiv preprint.
Proper citation: RRID:Addgene_209546 Copy
Can't find your Plasmid?
We recommend that you click next to the search bar to check some helpful tips on searches and refine your search firstly. If you want to find a specific plasmid, it's easier to enter an RRID or an Addgene Catalog Number to search. You can refine the search results using Facets on the left side of the search results page. If you are on the table view, you can also search in a specific column by clicking the column title and enter the keywords.
If you still could not find your plasmid in the search results, please help us by registering it into the system — it's easy. Register it with Addgene.
Welcome to the RRID Resources search. From here you can search through a compilation of resources used by RRID and see how data is organized within our community.
You are currently on the Community Resources tab looking through categories and sources that RRID has compiled. You can navigate through those categories from here or change to a different tab to execute your search through. Each tab gives a different perspective on data.
If you have an account on RRID then you can log in from here to get additional features in RRID such as Collections, Saved Searches, and managing Resources.
Here is the search term that is being executed, you can type in anything you want to search for. Some tips to help searching:
You can save any searches you perform for quick access to later from here.
We recognized your search term and included synonyms and inferred terms along side your term to help get the data you are looking for.
If you are logged into RRID you can add data records to your collections to create custom spreadsheets across multiple sources of data.
Here are the sources that were queried against in your search that you can investigate further.
Here are the categories present within RRID that you can filter your data on
Here are the subcategories present within this category that you can filter your data on
If you have any further questions please check out our FAQs Page to ask questions and see our tutorials. Click this button to view this tutorial again.
