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Vector Backbone Description: Vector Backbone:pACEC3GH; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:29934571
Proper citation: RRID:Addgene_110084 Copy
Vector Backbone Description: Vector Backbone:pKO; Vector Types:Bacterial Knockout Generation; Bacterial Resistance:Hygromycin
Defining Citation: PMID:29934571
Proper citation: RRID:Addgene_110090 Copy
Species: Other
Genetic Insert: FnCpf1
Vector Backbone Description: Vector Backbone:pMV261; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Hygromycin
Defining Citation: PMID:30039929
Comments: The other inserted are "crRNA to ligD" and "hygromysin resistance gene". "crRNA to ligD" species is synthetic, promoter is Pj23119. Shuttle vector Mycobacterium smegmatis/E.coli
Proper citation: RRID:Addgene_104625 Copy
Vector Backbone Description: Vector Backbone:new backbone; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Hygromycin
Defining Citation: PMID:31175189
Proper citation: RRID:Addgene_126601 Copy
Vector Backbone Description: Vector Backbone:new backbone; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Hygromycin
Defining Citation: PMID:31175189
Proper citation: RRID:Addgene_126600 Copy
Vector Backbone Description: Backbone Size:5903; Vector Backbone:new backbone; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Hygromycin
Defining Citation: PMID:31175189
Proper citation: RRID:Addgene_126606 Copy
Species: Other
Genetic Insert: Cas9
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Hygromycin
Defining Citation: PMID:30217854
Proper citation: RRID:Addgene_117232 Copy
Species: M. smegmatis
Genetic Insert: gfp, hyg
Vector Backbone Description: Vector Backbone:pYS2; Vector Types:Deletion generation; Bacterial Resistance:Hygromycin
Defining Citation: PMID:24100088
Comments: Addgene QC Next-generation sequencing identified a sequence discrepancy relative to the Genbank file linked under Supplemental Documents. Plasmid is expected to function as described in the associated publication.
Proper citation: RRID:Addgene_117490 Copy
Species: Other
Genetic Insert: Htb2-GFP
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Yeast Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:30314480
Comments: Integration plasmid in the pGAP locus. Please note that there are some sequence discrepancies between the Addgene quality control sequence and the author-supplied Genbank sequence. The authors have noted that the discrepancies do NOT affect plasmid function.
Proper citation: RRID:Addgene_117669 Copy
Vector Backbone Description: Backbone Size:4649; Vector Backbone:pMEG_BB3_YL18H_AB; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:30698703
Comments: Contains 34 bp GoldenMOCS-Yali Linker AB
Proper citation: RRID:Addgene_117827 Copy
Species: A. niger
Genetic Insert: gRNA1 (CexA)
Vector Backbone Description: Backbone Marker:Sarkari et al. 2017; Vector Backbone:pMST665; Vector Types:; Bacterial Resistance:Hygromycin
Defining Citation: PMID:30553933
Proper citation: RRID:Addgene_118078 Copy
Vector Backbone Description: Backbone Marker:Matthias Steiger Martin Altvater; Backbone Size:2852; Vector Backbone:MST607; Vector Types:Yeast Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:30553933
Proper citation: RRID:Addgene_118077 Copy
Species: A. niger
Genetic Insert: gRNA2 (CexA)
Vector Backbone Description: Backbone Marker:Sarkari et al. 2017; Vector Backbone:pMST665; Vector Types:; Bacterial Resistance:Hygromycin
Defining Citation: PMID:30553933
Proper citation: RRID:Addgene_118079 Copy
Species: A. niger
Genetic Insert: pTPI1-cexA-tCYC1
Vector Backbone Description: Backbone Marker:Matthias Steiger; Vector Backbone:MST606; Vector Types:Yeast Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:30553933
Proper citation: RRID:Addgene_118075 Copy
Species: Synthetic
Genetic Insert: hygR_gp41-1 N-intein + hygR_gp41-1 C-intein
Vector Backbone Description: Vector Backbone:pBAD33 + pTrc99a; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:31672972
Comments: pSiMPlh_N should be cotransformed with pSiMPlh_C (or vice versa) in E. Coli to get a reconstituted functional HPT thereby conferring resistance against hygromycin. mRuby3 gene was amplified from a construct bought by us from Addgene (ID: 74252).
Please see the Sequences link for the individual plasmid sequences.
The sample from Addgene will have both the plasmids pSiMPlh_N + pSiMPlh_C in it for the interested
antibiotic resistance. Separating individual plasmid backbones will be possible via PCR. Agarose gel electrophoresis can be used to visualize the presence of both the plasmids. But, separating individual plasmids via gel electrophoresis, in our hands, always resulted in cross-contamination of the two backbones.
Proper citation: RRID:Addgene_134316 Copy
Genetic Insert: scbR
Vector Backbone Description: Backbone Size:5715; Vector Backbone:pSET152; Vector Types:Bacterial Expression, Synthetic Biology, Integrative into Streptomyces genome via serine integrase (like parent pSET152); Bacterial Resistance:Hygromycin
Defining Citation: PMID:29130169
Comments: Biarnes-Carrera, Marc, Chang Kwon Lee, Takuya Nihira, Rainer Breitling, and Eriko Takano. ‘Orthogonal Regulatory Circuits for Escherichia Coli Based on the γ-Butyrolactone System of Streptomyces Coelicolor’. ACS Synthetic Biology 7, no. 4 (2018): 1043–55. https://doi.org/10.1021/acssynbio.7b00425.
pTE1062 is replicative in E. coli via pMB1 type origin, and carrying oriT (RP4) for delivery to Streptomyces by conjugation. In Streptomyces, pTE1062 is not replicative, and therefore selection is on integration into the genome mediated by phiC31 integrase.
Proper citation: RRID:Addgene_172356 Copy
Species: Homo sapiens
Genetic Insert: PIK3C3 and PIK3R4
Vector Backbone Description: Backbone Marker:Invivogen; Backbone Size:6321; Vector Backbone:pVitro2; Vector Types:Mammalian Expression, bicistronic; Bacterial Resistance:Hygromycin
Defining Citation: PMID:18957027
Proper citation: RRID:Addgene_24055 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Backbone Size:5582; Vector Backbone:pSMT3-S; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:20352111
Proper citation: RRID:Addgene_24659 Copy
Species: Synthetic gene
Genetic Insert: Turbo
Vector Backbone Description: Backbone Size:5582; Vector Backbone:pSMT3-S; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:20352111
Proper citation: RRID:Addgene_24658 Copy
Species: Synthetis gene
Genetic Insert: tdTomato
Vector Backbone Description: Backbone Size:5582; Vector Backbone:pSMT3-S; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:20352111
Proper citation: RRID:Addgene_24657 Copy
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