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Genetic Insert: B. subtilis deltaTn7
Vector Backbone Description: Vector Backbone:pACYC; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30617347
Comments:
IMPORT NOTES: Special character found in field(Gene or insert Name)
Proper citation: RRID:Addgene_119266 Copy
Genetic Insert: B. subtilis attTn7
Vector Backbone Description: Vector Backbone:pACYC; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30617347
Proper citation: RRID:Addgene_119264 Copy
Genetic Insert: Cas9 from S. pyogenes
Vector Backbone Description: Vector Backbone:p15A, Chloramphenicol resistance; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:32995532
Proper citation: RRID:Addgene_119162 Copy
Species: Cochliobolus heterostrophus (Pgpda), Herpes simplex virus (HSVtk), Neurospora crassa (Ttub)
Genetic Insert: Negative selection marker HSVtk (Pgpda:HSVtk:Ttub)
Vector Backbone Description: Backbone Marker:Diego Orzaez; Backbone Size:2690; Vector Backbone:pUPD2 (Addgene #68161); Vector Types:Synthetic Biology, Domestication of DNA parts for GoldenBraid asembly; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29680684
Proper citation: RRID:Addgene_119710 Copy
Species: Penicillium chrysogenum
Genetic Insert: Terminator paf
Vector Backbone Description: Backbone Marker:Diego Orzaez; Backbone Size:2690; Vector Backbone:pUPD2 (Addgene #68161); Vector Types:Synthetic Biology, Domestication of DNA parts for GoldenBraid asembly; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29680684
Proper citation: RRID:Addgene_119714 Copy
Vector Backbone Description: Vector Backbone:pNG2; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29366319
Proper citation: RRID:Addgene_119893 Copy
Genetic Insert: Biotin ligase
Vector Backbone Description: Vector Backbone:pLEMO; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31021608
Proper citation: RRID:Addgene_119817 Copy
Genetic Insert: intB13 integrase casette
Vector Backbone Description: Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30853419
Proper citation: RRID:Addgene_120230 Copy
Species: Synthetic
Genetic Insert: SpCas9-NG
Vector Backbone Description: Vector Backbone:pdCas9-bacteria; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_120228 Copy
Species: Synthetic
Genetic Insert: SpCas9-VQR
Vector Backbone Description: Vector Backbone:pdCas9-bacteria; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Comments: The TetR protein coding sequence contains an F178S difference compared to the reference sequence that does not impact the function of the plasmid.
Proper citation: RRID:Addgene_120224 Copy
Species: Synthetic
Genetic Insert: SpCas9-NG
Vector Backbone Description: Vector Backbone:pdCas9-bacteria; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_120225 Copy
Species: Escherichia coli
Genetic Insert: CRISPR spacers targeting IS1, IS5, IS3
Vector Backbone Description: Backbone Size:9326; Vector Backbone:pCRISPathBrick; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31008359
Comments: Please visit https://www.biorxiv.org/content/early/2018/09/27/428029 for BioRxiv preprint.
Proper citation: RRID:Addgene_120425 Copy
Species: Escherichia coli
Genetic Insert: CRISPR spacers targeting IS1, IS5, IS3, IS150
Vector Backbone Description: Backbone Size:9326; Vector Backbone:pCRISPathBrick; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31008359
Comments: Please visit https://www.biorxiv.org/content/early/2018/09/27/428029 for BioRxiv preprint.
Proper citation: RRID:Addgene_120424 Copy
Species: Synthetic
Genetic Insert: red fluorescent protein (mCherry), codon-optimized for C. difficile
Vector Backbone Description: Backbone Size:6400; Vector Backbone:pRFP185; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25527559
Proper citation: RRID:Addgene_120812 Copy
Species: Synthetic
Genetic Insert: red fluorescent protein (mCherry), codon-optimized for C. difficile, multiple-cloning site for protein fusion
Vector Backbone Description: Backbone Size:6400; Vector Backbone:pRFP185; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25527559
Proper citation: RRID:Addgene_120813 Copy
Species: Synthetic
Genetic Insert: cyan fluorescent protein, codon-optimized for low GC organisms
Vector Backbone Description: Backbone Size:6400; Vector Backbone:pRFP185; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24727226
Proper citation: RRID:Addgene_120814 Copy
Genetic Insert: Pecf15_up436 (-60 to +20)
Vector Backbone Description: Vector Backbone:ColE2 plasmid; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30575748
Proper citation: RRID:Addgene_120893 Copy
Species: Synthetic
Genetic Insert: Noncoding sequences for CRISPR-Cas12g1
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:4327; Vector Backbone:pACYC184; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30523077
Comments: For more information, please visit us at https://arbor.bio/. For commercial use or questions, please contact us at [email protected].
Proper citation: RRID:Addgene_120880 Copy
Genetic Insert: empty TagGFP2
Vector Backbone Description: Vector Backbone:pLenti-Origene; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31015465
Proper citation: RRID:Addgene_121426 Copy
Vector Backbone Description: Backbone Size:6881; Vector Backbone:p15A; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27136077
Proper citation: RRID:Addgene_121624 Copy
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