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  • Bacterial Resistance:hygromycin (facet)


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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pACEC3GH (Hyg)
 
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RRID:Addgene_110084 Hygromycin PMID:29934571 Vector Backbone:pACEC3GH; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin 2026-08-15 01:01:36 0
pKOdeltagalK (Hyg)
 
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RRID:Addgene_110090 Hygromycin PMID:29934571 Vector Backbone:pKO; Vector Types:Bacterial Knockout Generation; Bacterial Resistance:Hygromycin 2026-08-15 01:01:36 0
pSBY3_ligD-HygR
 
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RRID:Addgene_104625 FnCpf1 Other Hygromycin PMID:30039929 The other inserted are "crRNA to ligD" and "hygromysin resistance gene". "crRNA to ligD" species is synthetic, promoter is Pj23119. Shuttle vector Mycobacterium smegmatis/E.coli Vector Backbone:pMV261; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Hygromycin FnCpf1 codon-optimized C.glutamicum 2026-08-15 01:00:46 0
pOSV808
 
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RRID:Addgene_126601 Hygromycin PMID:31175189 Vector Backbone:new backbone; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Hygromycin 2026-08-15 01:04:27 0
pOSV807
 
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RRID:Addgene_126600 Hygromycin PMID:31175189 Vector Backbone:new backbone; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Hygromycin 2026-08-15 01:04:27 0
pOSV806
 
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RRID:Addgene_126606 Hygromycin PMID:31175189 Backbone Size:5903; Vector Backbone:new backbone; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Hygromycin 2026-08-15 01:04:27 0
pCasKP-hph
 
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1+ mentions
RRID:Addgene_117232 Cas9 Other Hygromycin PMID:30217854 Vector Backbone:Unknown; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Hygromycin 2026-08-15 01:02:51 2
pYS2
 
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RRID:Addgene_117490 gfp, hyg M. smegmatis Hygromycin PMID:24100088 Addgene QC Next-generation sequencing identified a sequence discrepancy relative to the Genbank file linked under Supplemental Documents. Plasmid is expected to function as described in the associated publication. Vector Backbone:pYS2; Vector Types:Deletion generation; Bacterial Resistance:Hygromycin 2026-08-15 01:02:54 0
Htb2-GFP
 
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RRID:Addgene_117669 Htb2-GFP Other Hygromycin PMID:30314480 Integration plasmid in the pGAP locus. Please note that there are some sequence discrepancies between the Addgene quality control sequence and the author-supplied Genbank sequence. The authors have noted that the discrepancies do NOT affect plasmid function. Vector Backbone:Unknown; Vector Types:Yeast Expression; Bacterial Resistance:Hygromycin Contains the gene encoding the Histone Htb2 from S.cerevisiae tagged with a GFP at the C-terminal. The protein is expressed constituvely by the promoter pGAP. 2026-08-15 01:02:56 0
pMEG_BB3_YL18H_AB
 
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RRID:Addgene_117827 Hygromycin PMID:30698703 Contains 34 bp GoldenMOCS-Yali Linker AB Backbone Size:4649; Vector Backbone:pMEG_BB3_YL18H_AB; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Hygromycin 2026-08-15 01:02:57 0
pMST-710
 
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RRID:Addgene_118078 gRNA1 (CexA) A. niger Hygromycin PMID:30553933 Backbone Marker:Sarkari et al. 2017; Vector Backbone:pMST665; Vector Types:; Bacterial Resistance:Hygromycin 2026-08-15 01:03:00 0
BB3_arscen_URA3_Hygro
 
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RRID:Addgene_118077 Hygromycin PMID:30553933 Backbone Marker:Matthias Steiger Martin Altvater; Backbone Size:2852; Vector Backbone:MST607; Vector Types:Yeast Expression; Bacterial Resistance:Hygromycin 2026-08-15 01:03:00 0
pMST-711
 
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RRID:Addgene_118079 gRNA2 (CexA) A. niger Hygromycin PMID:30553933 Backbone Marker:Sarkari et al. 2017; Vector Backbone:pMST665; Vector Types:; Bacterial Resistance:Hygromycin 2026-08-15 01:03:00 0
pMST-1312
 
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RRID:Addgene_118075 pTPI1-cexA-tCYC1 A. niger Hygromycin PMID:30553933 Backbone Marker:Matthias Steiger; Vector Backbone:MST606; Vector Types:Yeast Expression; Bacterial Resistance:Hygromycin 2026-08-15 01:03:00 0
pSiMPlh_N + pSiMPlh_C
 
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RRID:Addgene_134316 hygR_gp41-1 N-intein + hygR_gp41-1 C-intein Synthetic Hygromycin PMID:31672972 pSiMPlh_N should be cotransformed with pSiMPlh_C (or vice versa) in E. Coli to get a reconstituted functional HPT thereby conferring resistance against hygromycin. mRuby3 gene was amplified from a construct bought by us from Addgene (ID: 74252). Please see the Sequences link for the individual plasmid sequences. The sample from Addgene will have both the plasmids pSiMPlh_N + pSiMPlh_C in it for the interested antibiotic resistance. Separating individual plasmid backbones will be possible via PCR. Agarose gel electrophoresis can be used to visualize the presence of both the plasmids. But, separating individual plasmids via gel electrophoresis, in our hands, always resulted in cross-contamination of the two backbones. Vector Backbone:pBAD33 + pTrc99a; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin P254Q in hygR_gp41-1 N-intein 2026-08-15 01:05:43 0
pTE1062
 
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RRID:Addgene_172356 scbR Hygromycin PMID:29130169 Biarnes-Carrera, Marc, Chang Kwon Lee, Takuya Nihira, Rainer Breitling, and Eriko Takano. ‘Orthogonal Regulatory Circuits for Escherichia Coli Based on the γ-Butyrolactone System of Streptomyces Coelicolor’. ACS Synthetic Biology 7, no. 4 (2018): 1043–55. https://doi.org/10.1021/acssynbio.7b00425. pTE1062 is replicative in E. coli via pMB1 type origin, and carrying oriT (RP4) for delivery to Streptomyces by conjugation. In Streptomyces, pTE1062 is not replicative, and therefore selection is on integration into the genome mediated by phiC31 integrase. Backbone Size:5715; Vector Backbone:pSET152; Vector Types:Bacterial Expression, Synthetic Biology, Integrative into Streptomyces genome via serine integrase (like parent pSET152); Bacterial Resistance:Hygromycin 2026-08-15 01:10:23 0
pVitro2-hygro-N-myc-hVps34/hVps15-C-V5-his
 
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RRID:Addgene_24055 PIK3C3 and PIK3R4 Homo sapiens Hygromycin PMID:18957027 Backbone Marker:Invivogen; Backbone Size:6321; Vector Backbone:pVitro2; Vector Types:Mammalian Expression, bicistronic; Bacterial Resistance:Hygromycin 2026-08-15 01:12:27 1
pCHERRY3
 
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10+ mentions
RRID:Addgene_24659 mCherry Synthetic Hygromycin PMID:20352111 Backbone Size:5582; Vector Backbone:pSMT3-S; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin 2026-08-15 01:12:34 20
pCHARGE3
 
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1+ mentions
RRID:Addgene_24658 Turbo Synthetic gene Hygromycin PMID:20352111 Backbone Size:5582; Vector Backbone:pSMT3-S; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin 2026-08-15 01:12:33 1
pASTA3
 
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1+ mentions
RRID:Addgene_24657 tdTomato Synthetis gene Hygromycin PMID:20352111 Backbone Size:5582; Vector Backbone:pSMT3-S; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin 2026-08-15 01:12:33 2

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