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Vector Backbone Description: Backbone Marker:Stover et al., 1991 Nature 351:456; Vector Backbone:pMV261; Vector Types:Bacterial Expression, Synthetic Biology, Mycobacteria replicating vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22279533
Comments: The plasmid lacks the pAL5000 origin of replication and does not replicate in mycobacteria.
Proper citation: RRID:Addgene_36253 Copy
Species: Homo sapiens
Genetic Insert: DOT1L (GeneScript Synthesized)
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7325; Vector Backbone:pET28-MHL (Addgene plasmid 26096); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: PDB: 3SX0. http://www.thesgc.org/structures/3SX0/
PDB: 5JUW. http://www.thesgc.org/structures/5JUW/
Proper citation: RRID:Addgene_36196 Copy
Species: Homo sapiens
Genetic Insert: ITCH
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7325; Vector Backbone:pET28-MHL (Addgene plasmid 26096); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: PDB: 3TUG. http://www.thesgc.org/structures/3TUG/
Proper citation: RRID:Addgene_36197 Copy
Species: Homo sapiens
Genetic Insert: HDAC6.DC
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20133936
Comments: Additional Reference:
Gao etal "Histone Deacetylase 6 Regulates Growth Factor-Induced Actin Remodeling and Endocytosis" MOLECULAR AND CELLULAR BIOLOGY, Dec. 2007, p. 8637–8647
Proper citation: RRID:Addgene_36189 Copy
Species: Homo sapiens
Genetic Insert: HDAC6..delta Buz
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20133936
Proper citation: RRID:Addgene_36190 Copy
Species: Homo sapiens
Genetic Insert: H2A
Vector Backbone Description: Vector Backbone:pmTurquoise2-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22434194
Comments: Please note that there is a 3bp mismatch between Addgene's quality control sequence and the provided sequence from the depositing lab. This mismatch should not affect function or localization.
Proper citation: RRID:Addgene_36207 Copy
Species: Homo sapiens
Genetic Insert: Golgi targeting sequence
Vector Backbone Description: Vector Backbone:pmTurquoise2-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22434194
Proper citation: RRID:Addgene_36205 Copy
Species: Homo sapiens
Genetic Insert: Sec16A
Vector Backbone Description: Backbone Marker:Clontech, then modified; Vector Backbone:pVenus-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17005010
Proper citation: RRID:Addgene_36157 Copy
Species: Homo sapiens
Genetic Insert: Sec16A
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEYFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17005010
Proper citation: RRID:Addgene_36156 Copy
Species: Homo sapiens
Genetic Insert: p150Glued
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16772339
Proper citation: RRID:Addgene_36154 Copy
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-1 (NOT pEGFP-N1); Vector Types:Fluorescent protein cassette for sub-cloning; Bacterial Resistance:Kanamycin
Comments: Plasmid carrying monomeric (A206K) EGFP for C-terminal tagging
Proper citation: RRID:Addgene_36409 Copy
Vector Backbone Description: Backbone Marker:Vieira and Messing, 1991; Backbone Size:3868; Vector Backbone:pUK21; Vector Types:Bacterial Expression, Suicide vector for R. rubrum; Bacterial Resistance:Kanamycin
Comments: Constructed by D. P. Lies and G. P. Roberts
Proper citation: RRID:Addgene_36328 Copy
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Plasmid carrying monomeric (A206K) EGFP for N-terminal tagging
Proper citation: RRID:Addgene_36412 Copy
Species: Homo sapiens
Genetic Insert: active BCR-related
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17116687
Comments: This is the ABR isoform A. The R683A N795A double mutant lacks GTPase activity towards RacGTP. Construct: Young-jin Cho. Also see PMID 20962234.
Proper citation: RRID:Addgene_36417 Copy
Species: Homo sapiens
Genetic Insert: breakpoint cluster region protein isoform 1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20962234
Comments: Also see PMID 17116687.
Proper citation: RRID:Addgene_36416 Copy
Species: Homo sapiens
Genetic Insert: active BCR-related
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEYFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17116687
Comments: Made by direct mutagenesis of EYFP-Abr using a Stratagene Quick Change kit. In Dbl, mutation of residues N673 and D674 completely abolished its activity as a GEF towards Rac. These residues are highly conserved among DH domains and are also conserved as residues N245/E246 in Abr. We therefore mutated these residues in Abr and introduced the mutant into the RAW 264.7 macrophage cell line. However, the construct’s expression did not cause morphological changes in these cells. constructed by Young-Jin Cho.
Proper citation: RRID:Addgene_36419 Copy
Species: Homo sapiens
Genetic Insert: breakpoint cluster region
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pECFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17116687
Comments: Made by mutagenesis directly on plasmid Bcr-ECFP using a Stratagene Quick Change kit. In Dbl, mutation of residues N673 and D674 completely abolished its activity as a GEF towards Rac. These residues are highly conserved among DH domains and are also conserved as residues N689/ E690 in Bcr. We therefore mutated these residues in Bcr and introduced the mutants into the RAW 264.7 macrophage cell line. However, the construct did not caused morphological changes in these cells. Constructed by Young-Jin Cho.
Proper citation: RRID:Addgene_36418 Copy
Species: Homo sapiens
Genetic Insert: autophagy-related protein 2 homolog A
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4731; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22219374
Proper citation: RRID:Addgene_36456 Copy
Species: Homo sapiens
Genetic Insert: USP37
Vector Backbone Description: Backbone Marker:SGC; Backbone Size:7328; Vector Backbone:pET28a-LIC (Addgene plasmid 26094); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: PDB: 3U12. http://www.thesgc.org/structures/3U12/
Proper citation: RRID:Addgene_36898 Copy
Species: Homo sapiens
Genetic Insert: DMTF1
Vector Backbone Description: Backbone Marker:SGC (Addgene plasmid 26096); Backbone Size:7325; Vector Backbone:pET28-MHL (Addgene plasmid 26096); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: PDB: 2LLK. http://www.thesgc.org/structures/2LLK/
Proper citation: RRID:Addgene_36895 Copy
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