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Species: Synthetic
Genetic Insert: ChR1-VChR1 Chimera
Vector Backbone Description: Backbone Size:5385; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21796121
Comments:
Proper citation: RRID:Addgene_35500 Copy
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:5624; Vector Backbone:pRS42x; Vector Types:Yeast 2 micron vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22670222
Comments: TRP1 has been modified to remove HindIII and XbaI sites without altering the amino acid sequence of the Trp1 protein.
Proper citation: RRID:Addgene_35466 Copy
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:5801; Vector Backbone:pRS42x; Vector Types:Yeast 2 micron vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22670222
Comments: HIS3 has been modified to remove HindIII, KpnI and PstI sites without altering the amino acid sequence of the His3 protein.
Proper citation: RRID:Addgene_35464 Copy
Species: Chlamydomonas reinhardtii
Genetic Insert: hChR2(C128S/D156A)-mCherry
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21796121
Comments: ChR2 variant harboring two amino acid substitutions which act to stabilize the conducting state of the channel to deactivate with a time constant of nearly 30 minutes following a brief pulse of activating blue light. Like previously published step function opsins, this stabilized step function opsin (SSFO) may be deactivated using yellow light (590nm). The stabilized open state of the channel allows for both lower power activation, meaning in some circumstances the light delivery system need not penetrate the brain, as well as for behavior in the absence of a tethered laser or other light delivery system.
Proper citation: RRID:Addgene_35504 Copy
Species: Chlamydomonas reinhardtii
Genetic Insert: hChR2(C128S/D156A)-EYFP
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:5605; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21796121
Comments: ChR2 variant harboring two amino acid substitutions which act to stabilize the conducting state of the channel to deactivate with a time constant of nearly 30 minutes following a brief pulse of activating blue light. Like previously published step function opsins, this stabilized step function opsin (SSFO) may be deactivated using yellow light (590nm). The stabilized open state of the channel allows for both lower power activation, meaning in some circumstances the light delivery system need not penetrate the brain, as well as for behavior in the absence of a tethered laser or other light delivery system.
Proper citation: RRID:Addgene_35503 Copy
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:5711; Vector Backbone:pRS32x; Vector Types:Yeast 2 micron vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22670222
Comments: URA3 has been modified to remove ApaI and PstI sites without altering the amino acid sequence of the Ura3 protein.
Proper citation: RRID:Addgene_35469 Copy
Species: Chlamydomonas reinhardtii
Genetic Insert: hChR2
Vector Backbone Description: Backbone Size:5385; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21796121
Comments: ChR2 variant harboring two amino acid substitutions which act to stabilize the conducting state of the channel to deactivate with a time constant of nearly 30 minutes following a brief pulse of activating blue light. Like previously published step function opsins, this stabilized step function opsin (SSFO) may be deactivated using yellow light (590nm). The stabilized open state of the channel allows for both lower power activation, meaning in some circumstances the light delivery system need not penetrate the brain, as well as for behavior in the absence of a tethered laser or other light delivery system.
Proper citation: RRID:Addgene_35501 Copy
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:5797; Vector Backbone:pRS32x; Vector Types:Yeast 2 micron vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22670222
Comments: HIS3 has been modified to remove HindIII, KpnI, and PstI sites without altering the amino acid sequence of the His3 protein.
Proper citation: RRID:Addgene_35463 Copy
Species: Chlamydomonas reinhardtii
Genetic Insert: hChR2
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:5605; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22179551
Comments:
Proper citation: RRID:Addgene_35508 Copy
Species: Chlamydomonas reinhardtii
Genetic Insert: hChR2
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:5605; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22179551
Proper citation: RRID:Addgene_35507 Copy
Species: Chlamydomonas reinhardtii
Genetic Insert: hChR2
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:5605; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22179551
Proper citation: RRID:Addgene_35509 Copy
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4886; Vector Backbone:pRS41x; Vector Types:Yeast centromere vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22670222
Comments: URA3 has been modified to remove ApaI and PstI sites without altering the amino acid sequence of the Ura3 protein.
Proper citation: RRID:Addgene_35456 Copy
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:6009; Vector Backbone:pRS41x; Vector Types:Yeast centromere vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22670222
Comments: LEU2 has been modified to remove ClaI, EcoRI and KpnI sites without altering the amino acid sequence of the Leu2 protein.
Proper citation: RRID:Addgene_35454 Copy
Species: Homo sapiens
Genetic Insert: MHC IIA
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17567956
Proper citation: RRID:Addgene_35687 Copy
Species: Homo sapiens
Genetic Insert: MHC IIA
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18971378
Proper citation: RRID:Addgene_35689 Copy
Species: Homo sapiens
Genetic Insert: Estrogen Receptor beta
Vector Backbone Description: Vector Backbone:pcDNA 3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20660297
Proper citation: RRID:Addgene_35562 Copy
Species: Homo sapiens
Genetic Insert: ERbeta
Vector Backbone Description: Vector Backbone:pSUPER puro; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19106194
Proper citation: RRID:Addgene_35561 Copy
Species: Homo sapiens
Genetic Insert: MRLC1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22136066
Comments: This plasmid is identical to the one published in Beach et al., 2011, except the tet-inducible promoter has been replaced with a constitutive CMV promoter.
Proper citation: RRID:Addgene_35681 Copy
Species: Homo sapiens
Genetic Insert: MRLC1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22136066
Comments: This plasmid is identical to the one published in Beach et al., 2011, except the tet-inducible promoter has been replaced with a constitutive CMV promoter.
Proper citation: RRID:Addgene_35680 Copy
Genetic Insert: none
Vector Backbone Description: Vector Backbone:NA; Vector Types:; Bacterial Resistance:None
Defining Citation: PMID:10610690
Comments: To be used with the following plasmids from the Matthews lab: pTARA (www.addgene.org/31491), pLS1 (www.addgene.org/31490), and (www.addgene.org/31492) pLS1/-11
Proper citation: RRID:Addgene_35609 Copy
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