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Species: Caenorhabditis elegans
Genetic Insert: tomm20::miniSOG
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:9228; Vector Backbone:pBSK; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29360035
Proper citation: RRID:Addgene_179540 Copy
Species: Caenorhabditis elegans
Genetic Insert: NLF-1::GFP
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:7441; Vector Backbone:pBSK; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23522043
Comments: Please note Addgene NGS identified 3 mutations in the exons- I51N (exon1), T39A, L233S (exon2). Depositor confirms mutations do not affect plasmid function.
Proper citation: RRID:Addgene_179773 Copy
Species: Caenorhabditis elegans
Genetic Insert: NLF-1::2XGFP
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:5089; Vector Backbone:pBSK; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23522043
Comments: Please note Addgene NGS found some differences in genomic sequence of NLF-1. Depositor confirms this does not affect plasmid function.
Proper citation: RRID:Addgene_179771 Copy
Species: Caenorhabditis elegans
Genetic Insert: lin-41 miRNA target sequence
Vector Backbone Description: Backbone Marker:Available at Addgene (plasmid 12178); Backbone Size:5264; Vector Backbone:pIS0; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14697198
Comments: Used to test effects of lin-41 microRNA (let-7).
Proper citation: RRID:Addgene_11906 Copy
Species: Caenorhabditis elegans
Genetic Insert: BioID (BirA mutant)
Vector Backbone Description: Backbone Size:6954; Vector Backbone:Bluescript; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30125270
Proper citation: RRID:Addgene_118223 Copy
Species: Caenorhabditis elegans
Genetic Insert: mex-5
Vector Backbone Description: Backbone Marker:Askjaer lab; Vector Backbone:pBN338; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32550382
Proper citation: RRID:Addgene_109326 Copy
Species: Caenorhabditis elegans
Genetic Insert: End-1
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2; Vector Types:xenopus, mammalian, avian, and zebrafish; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10760276
Proper citation: RRID:Addgene_11030 Copy
Species: Caenorhabditis elegans
Genetic Insert: C113S-KVS-1
Vector Backbone Description: Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19850480
Comments: This plasmid contains a K861I mutation in KVS-1 compared to the reference sequence AAO65852.1.
Proper citation: RRID:Addgene_113062 Copy
Species: Caenorhabditis elegans
Genetic Insert: K+ voltage sensitive channel subunit 1
Vector Backbone Description: Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16222231
Comments: This plasmid contains a K861I mutation in KVS-1 compared to the reference sequence AAO65852.1.
Proper citation: RRID:Addgene_113061 Copy
Species: Caenorhabditis elegans
Genetic Insert: C113S K+ voltage sensitive channel subunit 1.2
Vector Backbone Description: Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19330004
Comments: This plasmid contains K681I and T208A mutations in KVS-1 compared the reference AAO65852.1 that do not impact function.
Proper citation: RRID:Addgene_113068 Copy
Species: Caenorhabditis elegans
Genetic Insert: MiRP K+ channel accessory Subunit 3
Vector Backbone Description: Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15799965
Comments: This plasmid contains a T61A mutation compared to the reference NP_490778.1 that does not impact function.
Proper citation: RRID:Addgene_113071 Copy
Species: Caenorhabditis elegans
Genetic Insert: C113S K+ voltage sensitive channel subunit 1
Vector Backbone Description: Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19330004
Proper citation: RRID:Addgene_113070 Copy
Species: Caenorhabditis elegans
Genetic Insert: nud-1
Vector Backbone Description: Backbone Marker:L4440 is available at Addgene; Backbone Size:2790; Vector Backbone:L4440; Vector Types:Worm Expression, RNAi; Bacterial Resistance:Ampicillin
Comments: Full length cDNA for C. elegans open-reading frame F53A2.4, encoding the nud-1 gene, cloned into RNAi feeding vector L4440. Can be used for RNAi feeding; should result in embryonic lethality at high levels of induction.
This plasmid is intended for use exclusively as a teaching resource as part of the Integrated Genomics Discovery-Based Laboratory Course. Addgene does not make any guarantee that the plasmid is suitable for research purposes.
Proper citation: RRID:Addgene_11337 Copy
Species: Caenorhabditis elegans
Genetic Insert: nud-1
Vector Backbone Description: Backbone Marker:see pLexA map in "author's map"; Backbone Size:5700; Vector Backbone:pLexA; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: Full-length cDNA for C. elegans open-reading frame F53A2.4, encoding the nud-1 gene, cloned into the yeast two-hybrid DNA-binding domain vector pLexA. This vector can be used as the bait for a yeast two-hybrid screen. See "author's map" for picture of empty pLexA vector.
Please note that Addgene's sequencing result differs from GenBank ID NM_067348, which indicates mutations in nud-1 are present at K136R, C220G and E235G.
This plasmid is intended for use exclusively as a teaching resource as part of the Integrated Genomics Discovery-Based Laboratory Course. Addgene does not make any guarantee that the plasmid is suitable for research purposes.
Proper citation: RRID:Addgene_11339 Copy
Species: Caenorhabditis elegans
Genetic Insert: lis-1
Vector Backbone Description: Backbone Marker:see pLexA map in "author's map"; Backbone Size:5700; Vector Backbone:pLexA; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: Full-length cDNA for C. elegans open-reading frame T03F6.5, encoding the lis-1 gene, cloned into the yeast two-hybrid DNA-binding domain vector pLexA. This vector can be used as the bait for a yeast two-hybrid screen. See "author's map" for picture of empty pLexA vector.
This plasmid is intended for use exclusively as a teaching resource as part of the Integrated Genomics Discovery-Based Laboratory Course. Addgene does not make any guarantee that the plasmid is suitable for research purposes.
Proper citation: RRID:Addgene_11338 Copy
Species: Caenorhabditis elegans
Genetic Insert: mNeptune
Vector Backbone Description: Backbone Marker:Anuj Sharma/Leifer Lab; Vector Backbone:pAS2; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_124332 Copy
Species: Caenorhabditis elegans
Genetic Insert: mNeptune
Vector Backbone Description: Backbone Marker:Anuj Sharma/Leifer Lab; Vector Backbone:pAS1; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_124331 Copy
Species: Caenorhabditis elegans
Genetic Insert: gpa-2, Cerulean
Vector Backbone Description: Backbone Marker:Anuj Sharma/Leifer Lab; Vector Backbone:pAS1; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_124337 Copy
Species: Caenorhabditis elegans
Genetic Insert: tagBFP2
Vector Backbone Description: Backbone Marker:Anuj Sharma/Leifer Lab; Vector Backbone:pAS1; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_124321 Copy
Species: Caenorhabditis elegans
Genetic Insert: tagBFP
Vector Backbone Description: Backbone Marker:Anuj Sharma/Leifer Lab; Vector Backbone:pAS2; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_124320 Copy
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