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Species: Danio rerio
Genetic Insert: wnt-8
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:2900; Vector Backbone:pGem-4; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Insert: RACE'd wnt-8. Wnt-8 in situ clone 25. Sense probe: cut with XbaI, transcribe with SP6.
Antisense probe: cut with EcoR1, transcribe with T7.
Plasmid History:
Constructed by Greg Kelly, 10/93.
Notes for Use:
Long 5' end of wnt-8 good in situ probe.
Proper citation: RRID:Addgene_16941 Copy
Species: Danio rerio
Genetic Insert: antiCD19-Notch3-GL4VP64
Vector Backbone Description: Vector Backbone:pHR; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32002793
Proper citation: RRID:Addgene_169916 Copy
Species: Danio rerio
Genetic Insert: slc1a3b
Vector Backbone Description: Backbone Size:3299; Vector Backbone:pT2A; Vector Types:Zebrafish; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32895565
Proper citation: RRID:Addgene_170208 Copy
Species: Danio rerio
Genetic Insert: slc1a3b
Vector Backbone Description: Backbone Size:3299; Vector Backbone:pT2A; Vector Types:Zebrafish; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32895565
Proper citation: RRID:Addgene_170209 Copy
Species: Danio rerio
Genetic Insert: wnt-1
Vector Backbone Description: Backbone Marker:Doug Melton Lab (Addgene #15030); Backbone Size:3000; Vector Backbone:SP64T; Vector Types:; Bacterial Resistance:Ampicillin
Comments: Insert: wnt-1 myc.
Insert size: 1.2 Kb.
Transcription: linearize with Pst1, transcribe with Sp6.
Plasmid History:
Constructed by Greg Kelly on date of 1/27/93, page 20 Book 2.
Notes for Use:
There is also a construct where wnt-1 myc is clone in antisense orientation.
Proper citation: RRID:Addgene_17042 Copy
Species: Danio rerio
Genetic Insert: wnt-4 cDNA
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pT7TS; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8541205
Comments: Insert: wnt-4 (zebrafish) cDNA.
Insert size: 1.3 Kb.
To make an in situ probe, linearize with EcoRV and transcribe with SP6.
To make sense RNA, linearize with Xba1 and transcribe with T7.
Plasmid History:
Constructed by Anne Ungar on date of 6/8/93.
Species of Sequence Origin: Zebrafish.
Notes for Use:
Note presence of R1, Sma, BamH1 sites brought in from pBSSK during construction. The 1274 nucleotide Nsi1 fragment containing the complete wnt-4 coding region was ligated into the Pst1 site of pBSSK (zf4 in pBSSK clone 5). The 5' end is near the Kpn1 site. A 1.3 Kb fragment was cut out with EcoRV and Spe1, isolated and ligated into EcoRV/Spe1 cut pT7Ts.
Citation: Ungar et al., Mechanisms of Development 52 (1995)
Proper citation: RRID:Addgene_17043 Copy
Species: Danio rerio
Genetic Insert: tiggywinkle hedgehog
Vector Backbone Description: Backbone Size:0; Vector Backbone:NA; Vector Types:; Bacterial Resistance:Ampicillin
Comments: twhh under control of CMV promoter.
Proper citation: RRID:Addgene_17060 Copy
Species: Danio rerio
Genetic Insert: wnt8 pBand3-5
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3000; Vector Backbone:pGemT easy; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11703928
Comments: RT PCR prodct ligated into vector full length cDNA cloned includes ORF1, IRES and ORF2.
For antiense RNA: transcribe with SP6 polymerase.
Plasmid History:
Species of Sequence Origin: Zebrafish.
Reference: Lekven, Dev Cell, 2001.
Proper citation: RRID:Addgene_17053 Copy
Species: Danio rerio
Genetic Insert: pax b
Vector Backbone Description: Backbone Size:3000; Vector Backbone:SP64T; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7586754
Comments: Insert: pax b sense or antisense orientation.
Insert size: 1.18 Kb.
Transcription: linearize at EcoR1, transcribe with SP6.
Plasmid History:
Constructed by Greg Kelly on date of 9/21/92, page 118 Book 1.
Sepecies of Sequence Origin: Zebrafish.
Note for Use:
This construct is used to make DIG probes or for making RNA suitable for injections.
Citation: Kelly and Moon, Developmental Genetics (1995)
Proper citation: RRID:Addgene_17056 Copy
Species: Danio rerio
Genetic Insert: wnt8
Vector Backbone Description: Backbone Size:4350; Vector Backbone:pCS2+MT; Vector Types:; Bacterial Resistance:Ampicillin
Comments: ORFs ligated into BamHI, ClaI cut pCS2+MT. Adds 6 myc tags onto carboxy terminus of each.
For Sense RNA: cut with Asp718 I and transcribe with SP6 polymerase.
Plasmid History:
Species of Sequence Origin: Zebrafish.
Proper citation: RRID:Addgene_17050 Copy
Species: Danio rerio
Genetic Insert: wnt8 ORF1/2
Vector Backbone Description: Backbone Size:4350; Vector Backbone:pCS2+MT; Vector Types:; Bacterial Resistance:Ampicillin
Comments: wnt8 ORFs + IREs cloned into BamHI-ClaI sites of pCS2+MT. Adds 6 myc tags onto c-terminus of ORF2.
For Sense RNA: cut with Asp718 I and transcribe with SP6 polymerase.
Plasmid History:
Species of Sequence Origin: Zebrafish.
Proper citation: RRID:Addgene_17052 Copy
Species: Danio rerio
Genetic Insert: wnt8
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2P+ (modified pCS2+); Vector Types:; Bacterial Resistance:Ampicillin
Comments: ORF1 amplified from pBand 3-5 (full length cDNA clone) ligated into BamHI-StuI cut CS2P+.
For Sense RNA: cut with Asp718 I and transcribe with SP6 polymerase.
Plasmid History:
Species of Sequence Origin: Zebrafish.
Proper citation: RRID:Addgene_17048 Copy
Species: Danio rerio
Genetic Insert: wnt8
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2P+ (modified pCS2+); Vector Types:; Bacterial Resistance:Ampicillin
Comments: ORF2 amplified from pBand 3-5 ligated into BamHI-StuI cut CS2P+.
For Sense RNA: cut with Asp718 I and transcribe with SP6 polymerase.
Plasmid History:
Species of Sequence Origin: Zebrafish.
Proper citation: RRID:Addgene_17049 Copy
Species: Danio rerio
Genetic Insert: Prickle-1
Vector Backbone Description: Backbone Size:4100; Vector Backbone:CS2P+; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12699626
Comments: Description: Zebrafish prickle-1 (full-length ORF), cloned into the BamH1 and EcoR1 sites of CS2P+.
Species of Sequence Origin: zebrafish.
Notes for Use: Has internal Not1, Kp1 sites, so linearize with BssH11 and transcribe SP6 for sense RNA. Select clones with amp.
Reference: MT Veeman et al., Current Biology 13: 680-685 (2003).
Proper citation: RRID:Addgene_17072 Copy
Species: Danio rerio
Genetic Insert: Prickle-1
Vector Backbone Description: Backbone Size:0; Vector Backbone:CS2+GFP-BB; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12699626
Comments: Description: Zebrafish prickle-1, cloned into the vector CS2+GFP-BB, to provide an N-terminal GFP. The C-terminal prenylation motif KNCIIS was deleted by PCR.
Species of Sequence Origin: zebrafish.
Notes for Use: Has internal Not1, Kp1 sites, so linearize with BssH11 and transcribe SP6 for sense RNA. Select clones with amp.
Reference: MT Veeman et al., Current Biology 13: 680-685 (2003).
Proper citation: RRID:Addgene_17074 Copy
Species: Danio rerio
Genetic Insert: vox-2
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2+; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin
Comments: Zvox-2 coding region amplified by PCR and inserted into CS2+ (first cloned into SK as zV93).
For RNA: cut with Asp718 and transcribe with SP6.
Plasmid History:
Species of Sequence Origin: Zebrafish.
Constructed by: David Kimelman, 6/28/97 XIX p. 67.
Proper citation: RRID:Addgene_17064 Copy
Species: Danio rerio
Genetic Insert: Stbm
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2+; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11780127
Comments: 6-c-myc epitopes in frame at the amino terminus of wild type zebrafish strabismus; EcoRI-Xba1 insert.
For Sense RNA: linearize with ASP 718 and transcribe with SP6 polymerase.
Plasmid History:
Species of Sequence Origin: Zebrafish.
Reference: M. Park and R. Moon, Nature Cell Biology 4, 20-25, 2002.
Proper citation: RRID:Addgene_17068 Copy
Species: Danio rerio
Genetic Insert: Dapper-1
Vector Backbone Description: Backbone Size:4100; Vector Backbone:CS2P+; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15539487
Comments: Dishevelled associated protein. cloned into Cla1/Xba1 sites.
Species of Sequence Origin: zebrafish.
Notes for Use: For functional synthetic RNA cut NotI, transcribe SP6.
Reference: Waxman JS, Hocking AM, Stoick CL, Moon RT.
Zebrafish Dapper1 and Dapper2 play distinct roles in Wnt-mediated developmental processes.
Development. 2004 Dec;131(23):5909-21.
Proper citation: RRID:Addgene_17082 Copy
Species: Danio rerio
Genetic Insert: Nlk1
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2+; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15151990
Comments: Description: Zebrafish Nemo-like kinase 1(NLK-1), cloned into the vector CS2+. 1425bp EcoR1-Xba1 insert.
Species of Sequence Origin: zebrafish.
Notes for Use: For functional synthetic RNA, linearize with Asp 718 and transcribe with SP6. Select clones with amp.
Reference: C. J. Thorpe and R. T. Moon, Development 131:2899-2909 (2004).
Proper citation: RRID:Addgene_17075 Copy
Species: Danio rerio
Genetic Insert: Nlk1
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pCS2+; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15151990
Comments: Description: Zebrafish Nemo-like kinase 1(NLK-1), cloned into the vector CS2+. ~1425bp EcoR1-Xba1 insert.
Clone is an kinase dead mutant of NLK1, analogous to K155M mutant of mouse NLK.
Species of Sequence Origin: zebrafish.
Notes for Use: For functional synthetic RNA, linearize with Asp 718 and transcribe with SP6. Select clones with amp.
Reference: C. J. Thorpe and R. T. Moon, Development 131:2899-2909 (2004).
Proper citation: RRID:Addgene_17078 Copy
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