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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pCFD2-dU6:2gRNA Resource Report Resource Website 1+ mentions |
RRID:Addgene_49409 | dU6-2:gRNA | Drosophila melanogaster | Ampicillin | PMID:25002478 | Please acknowledge Fillip Port and Simon Bullock when publishing work derived from use of this plasmid. Visit crisprflydesign.org for more information. | Backbone Marker:Norbert Perrimon, Jian-Quan Ni, Harvard; Vector Backbone:pValium22; Vector Types:Insect Expression, CRISPR; Bacterial Resistance:Ampicillin | 2026-08-15 01:15:59 | 4 | |
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pCFD1-dU6:1gRNA Resource Report Resource Website 1+ mentions |
RRID:Addgene_49408 | dU6-1:gRNA | Drosophila melanogaster | Ampicillin | PMID:25002478 | Please acknowledge Fillip Port and Simon Bullock when publishing work derived from use of this plasmid. Visit crisprflydesign.org for more information. | Backbone Marker:Norbert Perrimon, Jian-Quan Ni, Harvard; Vector Backbone:pValium22; Vector Types:Insect Expression, CRISPR; Bacterial Resistance:Ampicillin | 2026-08-15 01:16:00 | 4 | |
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pCFD4-U6:1_U6:3tandemgRNAs Resource Report Resource Website 100+ mentions |
RRID:Addgene_49411 | dU6-1:gRNA | Drosophila melanogaster | Ampicillin | PMID:25002478 | Please acknowledge Fillip Port and Simon Bullock when publishing work derived from use of this plasmid. Visit crisprflydesign.org for more information. | Backbone Marker:Norbert Perrimon, Jian-Quan Ni, Harvard; Vector Backbone:pValium22; Vector Types:Insect Expression, CRISPR; Bacterial Resistance:Ampicillin | 2026-08-15 01:16:00 | 134 | |
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pAFW-Ago2 Resource Report Resource Website 1+ mentions |
RRID:Addgene_50554 | Argonaute2 | Drosophila melanogaster | Ampicillin | PMID:20605501 | Please note that when compared to GenBank reference sequence NP_648775.1, the Ago2 sequence in this plasmid is missing amino acids 49-54 (LQQPQQ). | Backbone Marker:The Drosophila Gateway™ Vector Collection; Vector Backbone:pAFW; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:16:09 | 3 | |
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pENTR-E2F1-PIP3A Resource Report Resource Website |
RRID:Addgene_37142 | E2F1-P!P3A | Drosophila melanogaster | Kanamycin | PMID:19081076 | Backbone Marker:Invitrogen; Backbone Size:2580; Vector Backbone:pENTR/D-TOPO; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin | I153, Y156, Y157 to Alanine | 2026-08-15 01:14:13 | 0 | |
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pCaShs-hopscotch Resource Report Resource Website |
RRID:Addgene_37299 | hopscotch | Drosophila melanogaster | Ampicillin | PMID:7796812 | Perrimon Lab plasmid #99 | Backbone Size:8900; Vector Backbone:pCaSpeR-hs; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:14 | 0 | |
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ovoD1 D1B2H (7.2 kb) Resource Report Resource Website |
RRID:Addgene_37282 | ovoD1 genomic DNA fragment | Drosophila melanogaster | Ampicillin | PMID:8306893 | Perrimon Lab plasmid #25 Please see associated publication for cloning details | Backbone Size:7815; Vector Backbone:pCaSpeR 2; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:14 | 0 | |
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orthodenticle Resource Report Resource Website |
RRID:Addgene_37285 | orthodenticle | Drosophila melanogaster | Ampicillin | PMID:1979296 | Perrimon Lab plasmid #46 | Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBlueScript; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:14 | 0 | |
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10XSTAT92E–luciferase Resource Report Resource Website 1+ mentions |
RRID:Addgene_37393 | SOCS36E enhancer fragment | Drosophila melanogaster | Ampicillin | PMID:16055650 | Perrimon Lab plasmid #446 A 441-bp genomic fragment in the enhancer of SOCS36E containing two potential STAT92E-binding sites was amplified by PCR, using five different sets of oligos: (1) CTGCAGGAACCACTCAGAGTGCCTGCGTGT (PstI), GAATTCATACAAAACTGTCTTAGGTGTTTA (EcoRI); (2) CTGCAGGAACCACTCAGAGTGCCTGCGTGT (PstI), CTGCAGATACAAAACTGTCTTAGGTGTTTA (PstI); (3) GAATTCGAACCACTCAGAGTGCCTGCGTGT (EcoRI), GAATTCATACAAAACTGTCTTAGGTGTTTA (EcoRI); (4) AGATCTGAACCACTCAGAGTGCCTGCGTGT (BglII), AGATCTATACAAAACTGTCTTAGGTGTTTA (BglII); (5) GCGGCCGCGAACCACTCAGAGTGCCTGCGTGT (NotI), GCGGCCGCATACAAAACTGTCTTAGGTGTTTA (NotI). Each amplified genomic fragment containing different restriction enzyme sites was sequentially subcloned into pUAST. The genomic fragment amplified using the first set of oligos was subcloned into the PstI/EcoRI sites of pUAST to generate 2XSTAT92E. The genomic fragment amplified using the second set of oligos was subcloned into the PstI site of 2XSTAT92E to generate 4XSTAT92E. The genomic fragment amplified using the third set of oligos was subcloned into the EcoRI site of 4XSTAT92E to generate 6XSTAT92E. The genomic fragment amplified using the fourth set of oligos was subcloned into the BglII site of 6XSTAT92E to generate 8XSTAT92E. Next, the hsp70 minimal promoter element was amplified from pUAST by PCR using oligos GCGGCCGCAGCGGAGACTCTAGCGAGCG (NotI) and CTCGAGAATTCCCTATTCAGAGTTCT (XhoI). This hsp70 minimal promoter was subcloned into the NotI/XhoI sites of 8XSTAT92E to generate 8XSTAT92E–hsp70. Again, the genomic fragment amplified using the fifth set of oligos was subcloned into the NotI site of 8XSTAT92E–hsp70 vector to generate 10XSTAT92E–hsp70. Finally, an XhoI/XbaI fragment containing the firefly luciferase gene from the pGL3–luciferase vector (Promega) was subcloned into the XhoI/XbaI sites of 10XSTAT92E–hsp70 to generate 10XSTAT92E–luciferase. | Backbone Size:8904; Vector Backbone:pUAST; Vector Types:Insect Expression, Luciferase, JAK/STAT reporter construct; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:14 | 2 | |
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ovoD1 D1B2NR (18 kb) Resource Report Resource Website |
RRID:Addgene_37280 | ovoD1 genomic DNA fragment | Drosophila melanogaster | Ampicillin | PMID:8306893 | Perrimon Lab plasmid #23 Please see associated publication for cloning details | Backbone Size:7815; Vector Backbone:pCaSpeR 2; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:14 | 0 | |
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pUAS-hopscotch Resource Report Resource Website |
RRID:Addgene_37301 | hopscotch | Drosophila melanogaster | Ampicillin | PMID:7796812 | Perrimon Lab plasmid #100 | Backbone Size:8904; Vector Backbone:pUAST; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:14 | 0 | |
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pCaShs-Tum Resource Report Resource Website |
RRID:Addgene_37303 | hopTum-1 | Drosophila melanogaster | Ampicillin | PMID:7796812 | Perrimon Lab plasmid #101 The pCaShs-Tum construct was generated by the replacement of a 220 bp SacII-BstEII fragment from pCaShs-hop (Addgene plasmid# 37299) with the same fragment of PCR-generated DNA from hopTum-1 hemizygous flies. This mutation is a G to A transversion at nucleotide 1641 resulting in the substitution of a Glu for a Gly at amino acid 341. | Backbone Marker:Addgene plasmid# 37299; Vector Backbone:pCaShs-hopscotch; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin | Tul-l mutation is a G to A transversion at nucleotide 1641 resulting in the substitution of a Glu for a Gly at amino acid 341 | 2026-08-15 01:14:14 | 0 |
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pUAS-Tum Resource Report Resource Website |
RRID:Addgene_37304 | hopTum-1 | Drosophila melanogaster | Ampicillin | PMID:7796812 | Perrimon Lab plasmid #102 The pUAS-Tum was made by the insertion of the NotI-XbaI fragment from pCaShs-Tum (Addgene plasmid# 37303) into pUAST. | Backbone Size:8904; Vector Backbone:pUAST; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin | Tul-l mutation is a G to A transversion at nucleotide 1641 resulting in the substitution of a Glu for a Gly at amino acid 341 | 2026-08-15 01:14:14 | 0 |
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pCaSpeR-4 porcupine (genomic DNA) Resource Report Resource Website 1+ mentions |
RRID:Addgene_37376 | porcupine | Drosophila melanogaster | Ampicillin | PMID:8985181 | Perrimon Lab plasmid #123 The EcoRI-XbaI genomic DNA covering the porc locus was cloned in pCaspeR4 to generate a genomic porc rescue construct. | Backbone Size:7855; Vector Backbone:pCaSpeR-4; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:14 | 1 | |
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porcupine Resource Report Resource Website |
RRID:Addgene_37377 | porcupine | Drosophila melanogaster | Ampicillin | PMID:8985181 | Perrimon Lab plasmid #124 | Vector Backbone:pNB40; Vector Types:; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:14 | 0 | |
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Renilla luciferase-Pol III Resource Report Resource Website 1+ mentions |
RRID:Addgene_37380 | PolIII-Renilla control reporter | Drosophila melanogaster | Ampicillin | PMID:16311596 | Perrimon Lab plasmid #414 The PolIII–Renilla luciferase control construct was generated by PCR amplification of a fragment (base pairs 43,224–43,389 of scaffold AE003823) of the promoter of the D. melanogaster RNA PolIII 128 subunit (RpIII128) with a 5' BglII and a 3' SpeI site and ligation of this fragment into the BglII and SpeI sites of pRL-null. | Backbone Marker:Promega; Backbone Size:3320; Vector Backbone:pRL-null; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:14 | 6 | |
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tout velou (ttv) Resource Report Resource Website |
RRID:Addgene_37401 | tout-velu | Drosophila melanogaster | Ampicillin | PMID:9665133 | Perrimon Lab plasmid #179 | Vector Backbone:pNB40; Vector Types:; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:14 | 0 | |
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pUASP YFP Rab39 DN Resource Report Resource Website |
RRID:Addgene_37745 | Rab39 | Drosophila melanogaster | Ampicillin | PMID:17409086 | Backbone Size:9939; Vector Backbone:pUASP; Vector Types:Insect Expression, Drosophila; Bacterial Resistance:Ampicillin | S23N (dominant negative) | 2026-08-15 01:14:16 | 0 | |
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pUASP YFP Rab35 WT Resource Report Resource Website |
RRID:Addgene_37741 | Rab35 | Drosophila melanogaster | Ampicillin | PMID:17409086 | Backbone Size:9939; Vector Backbone:pUASP; Vector Types:Insect Expression, Drosophila; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:16 | 0 | ||
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pUASp YFP Rab32 CA Resource Report Resource Website |
RRID:Addgene_37740 | Rab32 | Drosophila melanogaster | Ampicillin | PMID:17409086 | Backbone Size:9939; Vector Backbone:pUASp; Vector Types:Insect Expression, Drosophila; Bacterial Resistance:Ampicillin | Q79L (constitutive active) | 2026-08-15 01:14:16 | 0 |
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