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Species: Homo sapiens
Genetic Insert: Ngn3
Vector Backbone Description: Vector Backbone:pCLXEBR65; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21761975
Comments: pTF is an optimized TET-promoter, described in HGT 2011, Giry et al.
Proper citation: RRID:Addgene_114307 Copy
Species: Homo sapiens
Genetic Insert: GFP
Vector Backbone Description: Vector Backbone:pCLX; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_114309 Copy
Species: Homo sapiens
Genetic Insert: gp78c
Vector Backbone Description: Backbone Marker:Amersham/GE; Backbone Size:5000; Vector Backbone:pGEX-4T2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11724934
Proper citation: RRID:Addgene_11429 Copy
Species: Homo sapiens
Genetic Insert: MYL2 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR, Donor Template; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30956114
Comments: This plasmid has been used with locus-specific CRISPR/Cas9 in a two-step editing process to add a mEGFP tag to the C-terminus of human MYL2 in WTC human induced pluripotent stem cells by the Allen Institute for Cell Science. After initial HDR, stable expression of CAGGS-driven mCherry will indicate cells with integrated donor sequence. This selection cassette is designed to be removed with subsequent CRISPR/Cas9-mediated excision as previously described (https://doi.org/10.1101/342881). A linker of 3-21 amino acids will be produced after excision. To enrich for edited cells, see our protocol for fluorsecence-assisted cell sorting and subcloning of hiPSCs (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209).
For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/.
Please visit https://www.biorxiv.org/content/early/2018/06/09/342881 for the bioRxiv preprint.
Proper citation: RRID:Addgene_114414 Copy
Species: Homo sapiens
Genetic Insert: TNNI1 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR, Donor Template; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30956114
Comments: This plasmid has been used with locus-specific CRISPR/Cas9 in a two-step editing process to add a mEGFP tag to the C-terminus of human TNNI1 in WTC human induced pluripotent stem cells by the Allen Institute for Cell Science. After initial HDR, stable expression of hPGK-driven mCherry will indicate cells with integrated donor sequence. This selection cassette is designed to be removed with subsequent CRISPR/Cas9-mediated excision as previously described (https://doi.org/10.1101/342881). A linker of 3-21 amino acids will be produced after excision. To enrich for edited cells, see our protocol for fluorsecence-assisted cell sorting and subcloning of hiPSCs (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209).
For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/.
Please visit https://www.biorxiv.org/content/early/2018/06/09/342881 for the bioRxiv preprint.
Proper citation: RRID:Addgene_114411 Copy
Species: Homo sapiens
Genetic Insert: MLL-AF4 fusion gene
Vector Backbone Description: Backbone Marker:Rudolf Jaenisch, Addgene Plasmid #22075; Backbone Size:6602; Vector Backbone:AAVS1 SA-2A-puro-pA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29386396
Proper citation: RRID:Addgene_114380 Copy
Species: Homo sapiens
Genetic Insert: LMNB1 Homology Arms with mTagRFPT-linker
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR, Donor Template; Bacterial Resistance:Ampicillin
Comments: This plasmid has been used with locus-specific CRISPR/Cas9 to add a mTagRFP-T tag to the N-terminus of human LMNB1 in WTC human induced pluripotent stem cells by the Allen Institute for Cell Science. Linker (AA) sequence: SGLRSRAQAS. After protein tagging using this donor template plasmid and CRISPR/Cas9 reagents, transfected cells may exhibit varying intensity levels of fluorescence, likely due to editing precision. To obtain cells of uniform intensity levels, see our protocol for fluorescence-assisted cell sorting and subcloning of transfected cells (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/ .
Proper citation: RRID:Addgene_114403 Copy
Species: Homo sapiens
Genetic Insert: PYL-HDAC5 aa 1-275
Vector Backbone Description: Backbone Size:4977; Vector Backbone:pAAV-CMV-GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34494094
Proper citation: RRID:Addgene_114400 Copy
Species: Homo sapiens
Genetic Insert: NUP153 Homology Arms with mEGFP-linker
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR, Donor Template; Bacterial Resistance:Ampicillin
Comments: This plasmid has been used with locus-specific CRISPR/Cas9 to add a mEGFP tag to the N-terminus of human NUP153 in WTC human induced pluripotent stem cells by the Allen Institute for Cell Science. Linker (AA) sequence: YSDLE. After protein tagging using this donor template plasmid and CRISPR/Cas9 reagents, transfected cells may exhibit varying intensity levels of fluorescence, likely due to editing precision. To obtain cells of uniform intensity levels, see our protocol for fluorescence-assisted cell sorting and subcloning of transfected cells (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/ .
Proper citation: RRID:Addgene_114407 Copy
Species: Homo sapiens
Genetic Insert: SMC1A Homology Arms with linker-mEGFP
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR, Donor Template; Bacterial Resistance:Ampicillin
Comments: This plasmid has been used with locus-specific CRISPR/Cas9 to add a mEGFP tag to the C-terminus of human SMC1A in WTC human induced pluripotent stem cells by the Allen Institute for Cell Science. Linker (AA) sequence: LSSSGPSGS. After protein tagging using this donor template plasmid and CRISPR/Cas9 reagents, transfected cells may exhibit varying intensity levels of fluorescence, likely due to editing precision. To obtain cells of uniform intensity levels, see our protocol for fluorescence-assisted cell sorting and subcloning of transfected cells (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/ .
Proper citation: RRID:Addgene_114406 Copy
Species: Homo sapiens
Genetic Insert: LCOR
Vector Backbone Description: Vector Backbone:pVL FLAG/His; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29628311
Proper citation: RRID:Addgene_114450 Copy
Species: Homo sapiens
Genetic Insert: PPAR gamma 2
Vector Backbone Description: Backbone Marker:pBabe-zeo is available at Addgene; Backbone Size:5169; Vector Backbone:pBabe-zeo (aka: pBabe-bleo); Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Comments: Human PPARg2 (-10 to 1515 TAG) - insert was cut with XhoI and cloned into vector cut with SalI.
Proper citation: RRID:Addgene_11439 Copy
Species: Homo sapiens
Genetic Insert: RXR alpha
Vector Backbone Description: Backbone Marker:pBABE-puro is available at Addgene; Backbone Size:5169; Vector Backbone:pBABE puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_11441 Copy
Species: Homo sapiens
Genetic Insert: JARID2
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pCR8/GW/TOPO; Vector Types:Gateway Entry Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29628311
Proper citation: RRID:Addgene_114443 Copy
Species: Homo sapiens
Genetic Insert: PALI1
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pCR8/GW/TOPO; Vector Types:Gateway Entry Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29628311
Proper citation: RRID:Addgene_114442 Copy
Species: Homo sapiens
Genetic Insert: Frataxin
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript II KS; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11035806
Comments: (kahn lab plasmid name = FM Blue)
This plasmid was cloned before there was any consensus sequence for frataxin. There are 2 mutations with the currently available frataxin sequence - L140P R165C. The effect of these mutations is unknown.
Proper citation: RRID:Addgene_11443 Copy
Species: Homo sapiens
Genetic Insert: LCOR
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pCR8/GW/TOPO; Vector Types:Gateway Entry Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29628311
Proper citation: RRID:Addgene_114441 Copy
Species: Homo sapiens
Genetic Insert: Frataxin
Vector Backbone Description: Backbone Marker:pBABE-bleo/zeo is available at Addgene; Backbone Size:4888; Vector Backbone:pBABE bleo; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11035806
Proper citation: RRID:Addgene_11442 Copy
Species: Homo sapiens
Genetic Insert: PALI1
Vector Backbone Description: Vector Backbone:pLENTI EF1_ FLAG/HA; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29628311
Proper citation: RRID:Addgene_114445 Copy
Species: Homo sapiens
Genetic Insert: LCOR
Vector Backbone Description: Vector Backbone:pLENTI EF1_ FLAG/HA; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29628311
Proper citation: RRID:Addgene_114444 Copy
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