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Species: Mus musculus
Genetic Insert: BMAL
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22653727
Proper citation: RRID:Addgene_47351 Copy
Species: Mus musculus
Genetic Insert: BMAL
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22653727
Proper citation: RRID:Addgene_47352 Copy
Species: Mus musculus
Genetic Insert: BMAL
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22653727
Proper citation: RRID:Addgene_47346 Copy
Species: Mus musculus
Genetic Insert: BMAL
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22653727
Proper citation: RRID:Addgene_47344 Copy
Species: Mus musculus
Genetic Insert: BMAL
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22653727
Proper citation: RRID:Addgene_47349 Copy
Species: Mus musculus
Genetic Insert: Clock
Vector Backbone Description: Backbone Marker:sigma; Vector Backbone:pCMV103Xflag; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22653727
Proper citation: RRID:Addgene_47340 Copy
Species: Mus musculus
Genetic Insert: BMAL
Vector Backbone Description: Vector Backbone:pfastbac; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22653727
Proper citation: RRID:Addgene_47335 Copy
Species: Mus musculus
Genetic Insert: Clock
Vector Backbone Description: Backbone Marker:sigma; Vector Backbone:pCMV103Xflag; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22653727
Proper citation: RRID:Addgene_47338 Copy
Species: Mus musculus
Genetic Insert: TTC30A2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5546; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23810713
Proper citation: RRID:Addgene_47324 Copy
Species: Mus musculus
Genetic Insert: TTC30B
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5546; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23810713
Proper citation: RRID:Addgene_47325 Copy
Species: Mus musculus
Genetic Insert: TTC30A1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5546; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23810713
Proper citation: RRID:Addgene_47323 Copy
Species: Mus musculus
Genetic Insert: KIF17-short
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5546; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: This vector directs expression of neither of the current mouse RefSeq Kif17 variants, isoform 1 or isoform 2. Rather the vector expresses a cDNA which corresponds to a different alternatively spliced transcript encoding a still shorter isoform of 511 amino acids rather than the 1038 residues of isoform 1 or 710 of isoform 2. The first 400 residues of the “short” isoform are essentially identical to the amino-terminal motor domain of isoform 1 and 2. Based on studies of Kif17 truncations, the short form of mouse Kif17 is predicted to form dimers and to bind microtubules.
*Compared to the reference sequence, this expression vector expresses a protein with substitution of conserved glycine-152 with a serine in the motor domain of Kif17. Due to this mutation, the properties of the produced protein could be altered.
Proper citation: RRID:Addgene_47545 Copy
Species: Mus musculus
Genetic Insert: Jun
Vector Backbone Description: Backbone Size:4300; Vector Backbone:pCMV-Tag2A; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21196933
Proper citation: RRID:Addgene_47443 Copy
Species: Mus musculus
Genetic Insert: PMCA4b
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_47597 Copy
Species: Mus musculus
Genetic Insert: Notch3 Intracellular domain
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pCMX; Vector Types:Mammalian Expression, in vitro transcription and translation; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8951795
Proper citation: RRID:Addgene_47618 Copy
Species: Mus musculus
Genetic Insert: MEK1-DD
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:5062; Vector Backbone:pGEX-4T-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23233447
Proper citation: RRID:Addgene_47576 Copy
Species: Mus musculus
Genetic Insert: Notch1 intracellular domain
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:4900; Vector Backbone:pGEX-4t-3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10433920
Comments: Construct also called GST-1111.
Proper citation: RRID:Addgene_47612 Copy
Species: Mus musculus
Genetic Insert: E-Cadherin EC12
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5406; Vector Backbone:pET-22b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23923816
Comments: Plasmid contains E-Cadherin domains 1 and 2 (aa157-375, when compared to GenBank reference sequence NP_033994.1). Numbering of the mutations is relative to this sequence. The pro-peptide sequence that is normally cleaved during maturation was intentionally removed as part of the cloning strategy for expression in bacteria.
Proper citation: RRID:Addgene_47926 Copy
Species: Mus musculus
Genetic Insert: E-Cadherin EC12
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5406; Vector Backbone:pET-22b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23923816
Comments: Plasmid contains E-Cadherin domains 1 and 2 (aa157-375, when compared to GenBank reference sequence NP_033994.1). Numbering of the mutation is relative to this sequence. The pro-peptide sequence that is normally cleaved during maturation was intentionally removed as part of the cloning strategy for expression in bacteria.
Proper citation: RRID:Addgene_47924 Copy
Species: Mus musculus
Genetic Insert: CMYC
Vector Backbone Description: Backbone Size:5169; Vector Backbone:pBABE puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24717435
Comments: The mutations found during the QC process have no functional consequence.
Proper citation: RRID:Addgene_53178 Copy
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