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Species: Saccharomyces cerevisiae
Genetic Insert: TRPL3
Vector Backbone Description: Vector Backbone:pRS425; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28239415
Comments: Yeast gene fragments were amplified from CEN.PK strain. The relative sequences in plasmids are based on S288C genomic sequence.
Proper citation: RRID:Addgene_98305 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: TRPL3
Vector Backbone Description: Vector Backbone:pRS425; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28239415
Comments: Yeast gene fragments were amplified from CEN.PK strain. The relative sequences in plasmids are based on S288C genomic sequence.
Proper citation: RRID:Addgene_98304 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: PTEF2>ScERG20N127W>TRPL3>PTEF1>AcNES1>TRPL41B
Vector Backbone Description: Vector Backbone:pRS425; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29471044
Comments: Please set 1/1/2018 as the initial day available for public access. Yeast promoters and terminators were amplified from CEN.PK strain. The relative sequences in plasmids are based on S288C genom
Proper citation: RRID:Addgene_98306 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: TRPL3
Vector Backbone Description: Vector Backbone:pRS425; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29471044
Comments: Publicly available as of 1/1/2018. Yeast promoters and terminators were amplified from CEN.PK strain. The relative sequences in plasmids are based on S288C genome.
Proper citation: RRID:Addgene_98308 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: McIsaac 2014 P3 promoter
Vector Backbone Description: Backbone Size:3436; Vector Backbone:pFA6; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29525204
Comments: The P3 promoter sequence is from McIsaac, R. S., Gibney, P. A., Chandran, S. S., Benjamin, K. R., & Botstein, D. (2014). Synthetic biology tools for programming gene expression without nutritional perturbations in Saccharomyces cerevisiae. Nucleic Acids Research, 42(6), e48. http://doi.org/10.1093/nar/gkt1402
Proper citation: RRID:Addgene_99048 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Tropomyosin
Vector Backbone Description: Vector Backbone:pJC20; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17452625
Proper citation: RRID:Addgene_99356 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: POL30
Vector Backbone Description: Backbone Marker:Gietz & Sugino (1988) Gene 74:527-34; Backbone Size:3545; Vector Backbone:YIplac204; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22941627
Comments: The ORF is followed by the ADH1 transcription terminator; digest with Bsu36I for integration into TRP1. Please see secondary publication PMID: 19107412 for more information about this plasmid.
Proper citation: RRID:Addgene_99550 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Swi1 NQ-rich region (AA1-536)
Vector Backbone Description: Backbone Marker:ATCC; Vector Backbone:p416TEF1; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20679490
Proper citation: RRID:Addgene_99552 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Swi1 N-rich region (AA1-327)
Vector Backbone Description: Backbone Marker:ATCC; Vector Backbone:p415TEF1; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21379326
Proper citation: RRID:Addgene_99555 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SMT3
Vector Backbone Description: Backbone Marker:Gietz & Sugino (1988) Gene 74:527-34; Backbone Size:5741; Vector Backbone:YEplac181; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19107412
Comments: The ORF is followed by the ADH1 transcription terminator. Please see secondary publication PMID: 22941627 for more information about this plasmid.
Proper citation: RRID:Addgene_99536 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: UBI3(1-76)
Vector Backbone Description: Backbone Marker:Gietz & Sugino (1988) Gene 74:527-34; Backbone Size:5741; Vector Backbone:YEplac181; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22941627
Comments: The ORF is followed by the ADH1 transcription terminator.
Proper citation: RRID:Addgene_99539 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SMT3
Vector Backbone Description: Backbone Marker:Gietz & Sugino (1988) Gene 74:527-34; Backbone Size:5241; Vector Backbone:YEplac195; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19107412
Comments: The ORF is followed by the ADH1 transcription terminator. Please see secondary publication PMID: 22941627 for more information about this plasmid.
Proper citation: RRID:Addgene_99538 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: POL30
Vector Backbone Description: Backbone Marker:Gietz & Sugino (1988) Gene 74:527-34; Backbone Size:4293; Vector Backbone:YIplac128; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22941627
Comments: The ORF is followed by the ADH1 transcription terminator; digest with AflII, BbsI or XcmI for integration into LEU2. Please see secondary publication PMID: 19107412 for more information about this plasmid.
Proper citation: RRID:Addgene_99544 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: POL30
Vector Backbone Description: Backbone Marker:Gietz & Sugino (1988) Gene 74:527-34; Backbone Size:4293; Vector Backbone:YIplac128; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22941627
Comments: The ORF is followed by the ADH1 transcription terminator; digest with BbsI or XcmI for integration into LEU2. Please see secondary publication PMID: 19107412 for more information about this plasmid.
Proper citation: RRID:Addgene_99543 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: POL30
Vector Backbone Description: Backbone Marker:Gietz & Sugino (1988) Gene 74:527-34; Backbone Size:3797; Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22941627
Comments: The ORF is followed by 1129 bp of the POL30 3' region; digest with StuI for integration into URA3 or with PmlI for integration upstream of endogenous POL30 (for exchange of alleles by 5FOA selection.) Please see secondary publication PMID: 19107412 for more information about this plasmid.
Proper citation: RRID:Addgene_99546 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: POL30
Vector Backbone Description: Backbone Marker:Gietz & Sugino (1988) Gene 74:527-34; Backbone Size:4293; Vector Backbone:YIplac128; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22941627
Comments: The ORF is followed by the ADH1 transcription terminator; digest with BbsI or XcmI for integration into LEU2. Please see secondary publication PMID: 19107412 for more information about this plasmid.
Proper citation: RRID:Addgene_99545 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: AKAR4
Vector Backbone Description: Vector Backbone:pCDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28106114
Proper citation: RRID:Addgene_99869 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Mrt4
Vector Backbone Description: Vector Backbone:pAS2-1; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29054886
Proper citation: RRID:Addgene_99997 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Cka1
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29054886
Proper citation: RRID:Addgene_99979 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Cka2
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29054886
Proper citation: RRID:Addgene_99980 Copy
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