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Vector Backbone Description: Backbone Size:6143; Vector Backbone:pGRB2.1; Vector Types:C. glabrata expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23934995
Proper citation: RRID:Addgene_45319 Copy
Species: Escherichia coli
Genetic Insert: rpoA
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3993; Vector Backbone:pGEMEX1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23389035
Proper citation: RRID:Addgene_45398 Copy
Species: Bos taurus
Genetic Insert: Rhodopsin
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22855808
Proper citation: RRID:Addgene_45399 Copy
Species: Synthetic
Genetic Insert: eGFP
Vector Backbone Description: Vector Backbone:pGRB2.3; Vector Types:C. glabrata expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23934995
Proper citation: RRID:Addgene_45316 Copy
Species: Homo sapiens
Genetic Insert: PAI-RBP
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5700; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11001948
Comments: Our lab isolated the protein based on it's ability to bind to a region of the PAI-1 (plasminogen activator inhibitor type 1) 3'UTR that we had determined to be involved in the cAMP regulation of mRNA stability. A search of the database revealed that the cDNA for the protein had been cloned by the German Genome Project and dubbed hypothetical protein. They kindly provided us with the clone, which we subcloned into pET-15b for expression of the protein.
Subsequently, we discovered isoforms of the protein with or without 6 and/or 15 amino acid insertions. These variants are now subcloned into pCELF.
Proper citation: RRID:Addgene_45390 Copy
Species: e. coli
Genetic Insert: lac repressor
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4486; Vector Backbone:pBEST-Luc; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20576148
Proper citation: RRID:Addgene_45393 Copy
Genetic Insert: melibiose repressor
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4486; Vector Backbone:pBEST-Luc; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20576148
Proper citation: RRID:Addgene_45396 Copy
Species: Homo sapiens
Genetic Insert: MCOPS3
Vector Backbone Description: Vector Backbone:pWzl-neo-flag-DEST; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23764425
Proper citation: RRID:Addgene_45309 Copy
Species: Homo sapiens
Genetic Insert: 6 copies of hPAI-1 promoter -732/-721 mutated at -725/723
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5700; Vector Backbone:pGL2 basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9792626
Proper citation: RRID:Addgene_45388 Copy
Species: Aotus trivirgatus (douroucouli)
Genetic Insert: MAVS
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pFLAG-CMV2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22427742
Proper citation: RRID:Addgene_45380 Copy
Species: Mus musculus
Genetic Insert: Src-KD-UniRapR-mCerulean-myc
Vector Backbone Description: Vector Backbone:pUSE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23569285
Proper citation: RRID:Addgene_45382 Copy
Species: Homo sapiens
Genetic Insert: 6 copies of hPAI-1 promoter -732/-721 mutated at -730/-728
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5700; Vector Backbone:pGL2 basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9792626
Proper citation: RRID:Addgene_45385 Copy
Species: Homo sapiens
Genetic Insert: 6 copies of hPAI1 promoter -732/-721 mutated at -732/-731
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5600; Vector Backbone:pGL2 basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9792626
Proper citation: RRID:Addgene_45384 Copy
Species: Lagothrix lagotricha (brown woolly monkey)
Genetic Insert: MAVS
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pFLAG-CMV2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22427742
Proper citation: RRID:Addgene_45376 Copy
Species: Saguinus labiatus (red-chested mustached tamarin)
Genetic Insert: MAVS
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pFLAG-CMV2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22427742
Proper citation: RRID:Addgene_45378 Copy
Species: Callicebus moloch (Red-bellied titi)
Genetic Insert: MAVS
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pFLAG-CMV2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22427742
Proper citation: RRID:Addgene_45379 Copy
Species: Macaca mulatta (Rhesus monkey)
Genetic Insert: MAVS
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pFLAG-CMV2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22427742
Proper citation: RRID:Addgene_45372 Copy
Species: Mus musculus
Genetic Insert: PKA regulatory subunit RI beta tagged by mEGFP
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19447092
Proper citation: RRID:Addgene_45526 Copy
Species: Rattus norvegicus
Genetic Insert: PKA regulatory subunit RII beta
Vector Backbone Description: Backbone Marker:Karel Svoboda; Backbone Size:5900; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression, with chicken beta actin promoter and WPRE; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19447092
Proper citation: RRID:Addgene_45529 Copy
Species: Mus musculus
Genetic Insert: TRPM7
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5000; Vector Backbone:pcDNA4/TO (modified); Vector Types:Mammalian Expression, Tetracycline Inducible; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11385574
Comments: For the purpose of expressing LTRPC7 in eukaryotic cells, the depositing lab used PCR to produce an epitope tagged expression construct from two overlapping murine LTRPC7 clones. The LTRPC7 coding sequence was modified by removing the initiating methionine and replacing it with a sequence encoding a Kozak sequence, the FLAG tag and the additional sequence GCGGCCGCAT, and by placing a SpeI site just after the stop codon. These modifications result in an expressed protein which started with the following amino acid sequence: MGDYKDDDDKRPH followed by the murine LTRPC7 coding sequence starting at the second amino acid. This construct is expressed from the pcDNA4/TO vector which provides tetracycline-controlled expression from a CMV promotor.
Proper citation: RRID:Addgene_45482 Copy
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