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Species: Mus musculus
Genetic Insert: Pdgfra donor region including homology arms and T2A-H2B-eGFP
Vector Backbone Description: Vector Backbone:pbluescript; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29889212
Proper citation: RRID:Addgene_113120 Copy
Species: Escherichia coli
Genetic Insert: NusG
Vector Backbone Description: Backbone Marker:UC Berkeley MacroLab; Backbone Size:6500; Vector Backbone:1C; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30122535
Proper citation: RRID:Addgene_113124 Copy
Species: Homo sapiens
Genetic Insert: p21 Activated kinase 4
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:7350; Vector Backbone:pmirGLO Dual-Luciferase; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29983868
Proper citation: RRID:Addgene_113090 Copy
Species: Synthetic
Genetic Insert: GINKO1
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30652129
Proper citation: RRID:Addgene_113112 Copy
Species: Homo sapiens
Genetic Insert: RapGEF3
Vector Backbone Description: Backbone Marker:Clonetech; Backbone Size:4700; Vector Backbone:pEGFP-N3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11801596
Proper citation: RRID:Addgene_113110 Copy
Species: Mus musculus
Genetic Insert: Gata4 donor region including homology arms and eGFP
Vector Backbone Description: Vector Backbone:pbluescript; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29889212
Proper citation: RRID:Addgene_113115 Copy
Species: Mus musculus
Genetic Insert: Cdk9 donor region including homology arms and HaloTag
Vector Backbone Description: Vector Backbone:pbluescript; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29889212
Proper citation: RRID:Addgene_113113 Copy
Species: Mus musculus
Genetic Insert: Gata3 donor region including homology arms and T2A-H2B-eGFP
Vector Backbone Description: Vector Backbone:pbluescript; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29889212
Proper citation: RRID:Addgene_113119 Copy
Species: Mus musculus
Genetic Insert: Dppa1 donor region including homology arms and T2A-H2B-Halo
Vector Backbone Description: Vector Backbone:pbluescript; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29889212
Proper citation: RRID:Addgene_113118 Copy
Species: Mus musculus
Genetic Insert: Fgfr2 donor region including homology arms and T2A-H2B-Halo
Vector Backbone Description: Vector Backbone:pbluescript; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29889212
Proper citation: RRID:Addgene_113117 Copy
Vector Backbone Description: Vector Backbone:T444T; Vector Types:Bacterial Expression, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29924347
Comments: For plasmid usage, please see the associated publication: Sturm et al.
Highly efficient RNAi and Cas9-based auto-cloning systems for C. elegans research. Nucleic Acids Research, gky516, https://doi.org/10.1093/nar/gky516
Proper citation: RRID:Addgene_113082 Copy
Species: Caenorhabditis elegans
Genetic Insert: C113S-KVS-1
Vector Backbone Description: Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19850480
Comments: This plasmid contains a K861I mutation in KVS-1 compared to the reference sequence AAO65852.1.
Proper citation: RRID:Addgene_113062 Copy
Species: Caenorhabditis elegans
Genetic Insert: K+ voltage sensitive channel subunit 1
Vector Backbone Description: Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16222231
Comments: This plasmid contains a K861I mutation in KVS-1 compared to the reference sequence AAO65852.1.
Proper citation: RRID:Addgene_113061 Copy
Species: Caenorhabditis elegans
Genetic Insert: C113S K+ voltage sensitive channel subunit 1.2
Vector Backbone Description: Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19330004
Comments: This plasmid contains K681I and T208A mutations in KVS-1 compared the reference AAO65852.1 that do not impact function.
Proper citation: RRID:Addgene_113068 Copy
Species: E. coli
Genetic Insert: RFP
Vector Backbone Description: Vector Backbone:pBbE8K; Vector Types:Bacterial Expression, Cell-free; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29596741
Proper citation: RRID:Addgene_113109 Copy
Species: Caenorhabditis elegans
Genetic Insert: MiRP K+ channel accessory Subunit 3
Vector Backbone Description: Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15799965
Comments: This plasmid contains a T61A mutation compared to the reference NP_490778.1 that does not impact function.
Proper citation: RRID:Addgene_113071 Copy
Species: Caenorhabditis elegans
Genetic Insert: C113S K+ voltage sensitive channel subunit 1
Vector Backbone Description: Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19330004
Proper citation: RRID:Addgene_113070 Copy
Species: Homo sapiens
Genetic Insert: prostatic acid phosphatase isoform 1
Vector Backbone Description: Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23233530
Comments: The plasmid contains a V360A compared to the reference NP_001090.2 that is not of concern for function.
Proper citation: RRID:Addgene_113052 Copy
Species: Homo sapiens
Genetic Insert: human Kv2.1
Vector Backbone Description: Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_113056 Copy
Genetic Insert: 2 bulged bind sites for CXCR4 siRNA antisense
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4045; Vector Backbone:pRL-TK; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12600936
Comments: The end of the ORF and the beginning of the 3' UTR is (with the TAA
stop codon, followed by T, then the XbaI site TCTAGA):
AAATGAACAA TAA T TCTAGA GCGGCCGCT.
We digested the plasmids with XbaI, and ligated in the following:
ctaga ttccgagatatcggtaat gggcc.
This produced a modified vector, still containing an XbaI site, but
now also containing an ApaI site (GGGCCC), to allow for directional
cloning of 3' UTR inserts. The final vector contains, starting with
the stop codon TAA: TAA T Tctaga ttccgagatatcggtaat gggccC TAGA.
All UTR inserts were then constructed in the following format:
TCTAGA CTCGAGCCGG[binding site]ATCGC GGGCCC. A single perfect site
sequence is AAGTTTTCACTCCAGCTAACACCGG.
CXCR4 siRNA used is GUUUUCACUCCAGCUAACACATTCAAAAGUGAGGUCGAUUGU.
Proper citation: RRID:Addgene_11306 Copy
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