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Species: S. pyogenes
Genetic Insert: Cas9 nickase
Vector Backbone Description: Backbone Marker:Thermo-Fisher; Backbone Size:3900; Vector Backbone:PCR2-1 TOPO; Vector Types:in vitro transcription; Bacterial Resistance:Ampicillin and Kanamycin
Comments: The poly A tail included in the sequence is sufficient for purification of full length transcript using an oligo dT column. This decreases the amount of incomplete transcripts (and therefore decreases truncated Cas9 proteins).
Proper citation: RRID:Addgene_71311 Copy
Genetic Insert: sfGFP
Vector Backbone Description: Backbone Size:4719; Vector Backbone:pG1AK; Vector Types:Bacterial Expression, Synthetic Biology, Shuttle Vector; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:27332993
Comments: Plasmid from the Geobacillus plasmid set - a modular shuttle vector toolkit for thermophile metabolic engineering
Proper citation: RRID:Addgene_71739 Copy
Vector Backbone Description: Backbone Size:4719; Vector Backbone:pG1AK; Vector Types:Bacterial Expression, Synthetic Biology, Shuttle Vector; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:27332993
Comments: Plasmid from the Geobacillus plasmid set - a modular shuttle vector toolkit for thermophile metabolic engineering
Proper citation: RRID:Addgene_71736 Copy
Species: Escherichia coli str. K-12 substr. MG1655
Genetic Insert: response regulator in two-component regulatory system with EvgS
Vector Backbone Description: Backbone Size:3851; Vector Backbone:pDRIVE-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Comments: TA cloning product with 286 bp 5'-UTR including EvgA binding site.
Proper citation: RRID:Addgene_72467 Copy
Species: Acetobacter aceti 1023
Genetic Insert: DNA mismatch repair protein MutS (partial gene)
Vector Backbone Description: Backbone Size:3851; Vector Backbone:pDRIVE-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Comments: Used for nucleotide quantitation in RT-PCR and similar experiments for Acetobacter aceti 1023.
Based on method described in M. Ishikawa, A. Okamoto-Kainuma, T. Jochi, I. Suzuki, K. Matsui, T Kaga, Y. Koizumi (2009) Cloning and characterization of grpE in Acetobacter pasteurianus NBRC 3283. J. Biosci. Bioeng. 109(1), 25–31[doi: 10.1016/j.jbiosc.2009.07.008]
Proper citation: RRID:Addgene_72437 Copy
Species: Mus musculus
Genetic Insert: Semaphorin-4A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:mRNA In situ hybridization probe; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:21122816
Proper citation: RRID:Addgene_68029 Copy
Species: Mus musculus
Genetic Insert: Semaphorin-4F
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:mRNA In situ hybridization probe; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:21122816
Proper citation: RRID:Addgene_68033 Copy
Species: Mus musculus
Genetic Insert: Semaphorin-4D
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:mRNA In situ hybridization probe; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:21122816
Proper citation: RRID:Addgene_68032 Copy
Species: Mus musculus
Genetic Insert: Semaphorin-4C
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:mRNA In situ hybridization probe; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:21122816
Proper citation: RRID:Addgene_68031 Copy
Species: Mus musculus
Genetic Insert: Semaphorin-4B
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:mRNA In situ hybridization probe; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:21122816
Proper citation: RRID:Addgene_68030 Copy
Species: Gallus gallus
Genetic Insert: CAG-loxP-stop-loxP-RFP-ires-tTA-WPRE
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4339; Vector Backbone:pDisplay; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:24685175
Proper citation: RRID:Addgene_69138 Copy
Species: Mus musculus
Genetic Insert: TCR beta cDNA clone HSV2.3
Vector Backbone Description: Backbone Marker:Invitrogen ; Backbone Size:3947; Vector Backbone:pCRII; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:11940116
Comments: This plasmid contains the TCR beta chain cDNA from the HSV2.3 clone and was used to create the transgenic construct p3A9cbetaTCR.HSV2.3
(Addgene plasmid #69594).
Proper citation: RRID:Addgene_74077 Copy
Species: Synthetic
Genetic Insert: aphII
Vector Backbone Description: Backbone Marker:Alexeyev, M.F., BioTechniques 18:52-54, 1995; Vector Backbone:pBSL15; Vector Types:Bacterial vector based on pMB1 replicon; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:26921431
Proper citation: RRID:Addgene_74108 Copy
Species: maize (corn)
Genetic Insert: zmU3 promoter - gRNA
Vector Backbone Description: Backbone Marker:Transgen; Backbone Size:3929; Vector Backbone:pEasy-blunt; Vector Types:CRISPR, Plant expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:24576457
Proper citation: RRID:Addgene_53061 Copy
Species: S. pyogenes
Genetic Insert: Cas9
Vector Backbone Description: Backbone Size:7662; Vector Backbone:pPMQAK1; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:35969224
Proper citation: RRID:Addgene_190790 Copy
Species: Streptomyces lavendulae subsp. lavendulae CCM 3239
Genetic Insert: T5 terminator
Vector Backbone Description: Backbone Size:4065; Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:36173451
Proper citation: RRID:Addgene_190785 Copy
Species: pSA3239
Genetic Insert: upstream region of the aur1 cluster for auricin
Vector Backbone Description: Backbone Size:7907; Vector Backbone:sCos-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:36173451
Proper citation: RRID:Addgene_190786 Copy
Species: E. coli
Genetic Insert: lacZ
Vector Backbone Description: Vector Backbone:pXD69m2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:36494336
Proper citation: RRID:Addgene_191620 Copy
Species: E. coli
Genetic Insert: lacZ
Vector Backbone Description: Vector Backbone:pXD69m2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:36494336
Proper citation: RRID:Addgene_191621 Copy
Species: E. coli
Genetic Insert: lacZ
Vector Backbone Description: Vector Backbone:pXD69m2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:36494336
Proper citation: RRID:Addgene_191627 Copy
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