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Species: methanosarcina mazei
Genetic Insert: PylTUUA
Vector Backbone Description: Vector Backbone:pEVOL; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:22628069
Proper citation: RRID:Addgene_182537 Copy
Species: Pseudomonas aeruginosa
Genetic Insert: TetR/A
Vector Backbone Description: Backbone Marker:Brumwell et al.; Backbone Size:14930; Vector Backbone:pDEINO1; Vector Types:Synthetic Biology, Bacterial and Yeast Cloning; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:35254818
Proper citation: RRID:Addgene_179487 Copy
Species: Synthetic
Genetic Insert: Reverse CP25 promoter and forward rpoC terminator for dsRNA expression
Vector Backbone Description: Vector Backbone:ColE1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:32001655
Proper citation: RRID:Addgene_183127 Copy
Species: Synthetic
Genetic Insert: Reverse rnpB-T1 terminator and forward CP25 promoter for dsRNA expression
Vector Backbone Description: Vector Backbone:colE1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:32001655
Proper citation: RRID:Addgene_183126 Copy
Species: Bacteriophage MS2
Genetic Insert: MS2 coat protein
Vector Backbone Description: Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:33500316
Proper citation: RRID:Addgene_174786 Copy
Species: Escherichia coli
Genetic Insert: tdk
Vector Backbone Description: Vector Backbone:ColE1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:32232367
Comments: The tdk-KanR was decribed in Metzgar, David, Jamie M Bacher, Valérie Pezo, John Reader, Volker Döring, Paul Schimmel, Philippe Marlière, and Valérie de Crécy-Lagard. (2004) Acinetobacter Sp. ADP1: An Ideal Model Organism for Genetic Analysis and Genome Engineering. Nucleic Acids Research 32 (19): 5780–5790. https://doi.org/10.1093/nar/gkh881.
Proper citation: RRID:Addgene_180592 Copy
Species: Homo sapiens
Genetic Insert: Lucia Luciferase
Vector Backbone Description: Backbone Marker:Diego Orzaez Lab; Backbone Size:2343; Vector Backbone:pUPD3; Vector Types:Mammalian Expression, Luciferase, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31836712
Comments: Insert can be released with BsaI.
Synthetic luciferase from Invivogen, codon-optimized and synthesized de novo.
Proper citation: RRID:Addgene_135287 Copy
Genetic Insert: Cas12c single guide RNA targeting GFP
Vector Backbone Description: Vector Backbone:p15A ori; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:35659325
Proper citation: RRID:Addgene_183070 Copy
Species: Synthetic
Genetic Insert: Cas12c tracrRNA and pre-crRNA
Vector Backbone Description: Vector Backbone:p15A ori; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:35659325
Comments: A part of the Cas12c2 concatenated noncoding RNA sequence from Addgene plasmid # 120877 (http://n2t.net/addgene:120877 ; RRID:Addgene_120877) was used for tracrRNA expression.
Proper citation: RRID:Addgene_183076 Copy
Species: Synthetic
Genetic Insert: LbCas12a guide RNA containing BbsI sites in spacer sequence
Vector Backbone Description: Vector Backbone:p15A ori; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:35659325
Proper citation: RRID:Addgene_183222 Copy
Genetic Insert: Efflux pump
Vector Backbone Description: Backbone Size:4904; Vector Backbone:pBbS5c; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25496359
Comments: This plasmid carries G179V in Maqu_3494 compared to the genomic reference sequence. This mutation does not affect plasmid function.
Proper citation: RRID:Addgene_86205 Copy
Genetic Insert: Efflux pump
Vector Backbone Description: Backbone Size:4904; Vector Backbone:pBbS5c; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25496359
Proper citation: RRID:Addgene_86203 Copy
Species: Synthetic
Genetic Insert: Pveg-sLucopt
Vector Backbone Description: Backbone Marker:Robert Fagan and Neil Fairweather; Backbone Size:6378; Vector Backbone:pRPF185; Vector Types:Bacterial Expression, Luciferase, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Comments: Please note that the individual components of this construct (that is, Pveg and sLucopt) are described in Oliveira Paiva AM et al (2016) ACS Synth Biol (DOI: 10.1021/acssynbio.6b00104). During the cloning a mutation occurred that removes the SacI site in between the Pveg and sLucopt genes. The 5' (KpnI) and 3' (BamHI) restriction sites remain.
Proper citation: RRID:Addgene_86554 Copy
Vector Backbone Description: Backbone Size:5328; Vector Backbone:pBBR; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28931397
Proper citation: RRID:Addgene_86709 Copy
Genetic Insert: gltA1-gltA2-udhA-GapAP1 guide array
Vector Backbone Description: Backbone Marker:None - Constructed in House; Backbone Size:2500; Vector Backbone:pCASCADE; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_87151 Copy
Genetic Insert: gltA1-gltA2-gapAP1 guide array
Vector Backbone Description: Backbone Marker:None - Constructed in House; Backbone Size:2500; Vector Backbone:pCASCADE; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_87150 Copy
Genetic Insert: fabI-gltA1-gltA2-udhA-zwf-gapAP1 guide array
Vector Backbone Description: Backbone Marker:None - Constructed in House; Backbone Size:2500; Vector Backbone:pCASCADE; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_87149 Copy
Genetic Insert: gapAP1 guide array
Vector Backbone Description: Backbone Marker:None - Constructed in House; Backbone Size:2500; Vector Backbone:pCASCADE; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_87146 Copy
Species: Bacillus subtilis
Genetic Insert: XynA signal peptide
Vector Backbone Description: Backbone Size:2676; Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28230977
Proper citation: RRID:Addgene_87372 Copy
Genetic Insert: SpyTag
Vector Backbone Description: Backbone Size:2676; Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28230977
Proper citation: RRID:Addgene_87375 Copy
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