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Species: Synthetic
Genetic Insert: Codon-optimised RhopH3
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6633; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24043421
Proper citation: RRID:Addgene_47781 Copy
Species: Synthetic
Genetic Insert: promoter 14 and BCD9
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pFAB1781; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23474465
Comments: http://biofab.org/services
RFP fluorescence (Arbitrary Units, AU) is used to measure the efficiency of the promoter/BCD combination. Promoter/BCD combinations ranged from a low score of 6.14 to a high score of 634.54. The score for this combination was: 90.37
Proper citation: RRID:Addgene_47816 Copy
Species: Synthetic
Genetic Insert: Codon-optimised RH2b (partial)
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6633; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24043421
Proper citation: RRID:Addgene_47778 Copy
Species: Arabidopsis thaliana
Genetic Insert: AmTrac-100
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pDR GW; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23840931
Proper citation: RRID:Addgene_47771 Copy
Species: Arabidopsis thaliana
Genetic Insert: AmTrac
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pDR GW; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23840931
Proper citation: RRID:Addgene_47770 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: MepTrac-H194E
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pDR GW; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23840931
Proper citation: RRID:Addgene_47764 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: meptrac
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pDR GW; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23840931
Proper citation: RRID:Addgene_47768 Copy
Species: Arabidopsis thaliana
Genetic Insert: AmTrac-GS
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pDR GW; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23840931
Proper citation: RRID:Addgene_47769 Copy
Genetic Insert: PesaR-luxCDABE-term
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:2934; Vector Backbone:pET-17b; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23879176
Comments: PesaR-luxCDABE-transcriptional terminator fragment from pCS-PesaRlux is between XhoI and BglII sites in pET17b.
PesaR (274bp, indicated as lowercase letters in the 2nd partial sequence for PesaR between XhoI and BamHI) is located between XhoI and BamHI sites. luxCDABE is located between two NotI sites. The transcriptional terminator is located downstream of luxCDABE between the second NotI and BglII.
Sequencing primers provided were used to sequence PesaR.
Proper citation: RRID:Addgene_47802 Copy
Species: Arabidopsis thaliana
Genetic Insert: AmTrac1;2
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pDR GW; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23840931
Proper citation: RRID:Addgene_47766 Copy
Species: Arabidopsis thaliana
Genetic Insert: AmTrac-LS
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pDR GW; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23840931
Proper citation: RRID:Addgene_47767 Copy
Species: HPV
Genetic Insert: HPV 6bE6
Vector Backbone Description: Backbone Size:6550; Vector Backbone:pNCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22553330
Proper citation: RRID:Addgene_47800 Copy
Species: Homo sapiens
Genetic Insert: PMCA4a
Vector Backbone Description: Backbone Marker: Adamo, H.P. et al. (1992) Biochem. J. 285, 791-797; Vector Backbone:pMM2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8276828
Comments: Note that PMCA4 residue 2 is Ala (instead of Thr) due to an optimized initiation sequence (ATG GCG). The alternatively spliced 178 nt exon sequence is at positions 4377-4554.
Proper citation: RRID:Addgene_47761 Copy
Species: Homo sapiens
Genetic Insert: PMCA4b
Vector Backbone Description: Backbone Marker: Adamo, H.P. et al. (1992) Biochem. J. 285, 791-797; Vector Backbone:pMM2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:1323273
Comments: Note that PMCA4 residue 2 is Ala (instead of Thr) due to an optimized initiation sequence (ATG GCG).
Proper citation: RRID:Addgene_47759 Copy
Species: Synthetic
Genetic Insert: dCas9-VP64
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23892898
Comments: Plasmid Features:
Cas9 = 409-4512
NLS = 4519-4539
3X FLAG = 4546-4611
VP64 = 4633-4794
Proper citation: RRID:Addgene_47753 Copy
Species: Synthetic
Genetic Insert: codon optimized dCas9-VP64
Vector Backbone Description: Backbone Marker:Joung lab (Addgene plasmid # 43861); Vector Backbone:pJDS246; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23892898
Comments: Plasmid Features:
Cas9 = 409-4512
NLS = 4519-4539
3X FLAG = 4546-4611
VP64 = 4633-4794
Proper citation: RRID:Addgene_47754 Copy
Species: Homo sapiens
Genetic Insert: CITED2
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23082118
Proper citation: RRID:Addgene_47749 Copy
Species: Synthetic
Genetic Insert: Codon-optimised EBA140
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6633; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24043421
Proper citation: RRID:Addgene_47742 Copy
Species: Synthetic
Genetic Insert: Codon-optimised EBA175
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6633; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24043421
Proper citation: RRID:Addgene_47743 Copy
Species: Synthetic
Genetic Insert: Codon-optimised MSP9
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6633; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24043421
Proper citation: RRID:Addgene_47740 Copy
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