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Species: Synthetic
Genetic Insert: gRNA scaffold
Vector Backbone Description: Backbone Size:6500; Vector Backbone:pCambia; Vector Types:Plant Expression, plant binary vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25432517
Proper citation: RRID:Addgene_62200 Copy
Species: Synthetic
Genetic Insert: Luciferase
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Mammalian Expression, Luciferase, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25751063
Proper citation: RRID:Addgene_62168 Copy
Species: Synthetic
Genetic Insert: gRNA scaffold
Vector Backbone Description: Backbone Size:6500; Vector Backbone:pCambia; Vector Types:Plant Expression, plant binary vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25432517
Proper citation: RRID:Addgene_62201 Copy
Species: Synthetic
Genetic Insert: Puromycin
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25751063
Proper citation: RRID:Addgene_62162 Copy
Species: Synthetic
Genetic Insert: Puromycin
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25751063
Proper citation: RRID:Addgene_62164 Copy
Species: Mus musculus
Genetic Insert: Nxph4-2A-CreERT2
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:2850; Vector Backbone:pBluescriptII SK+; Vector Types:Mammalian Expression, Mouse Targeting, Cre/Lox; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25071457
Proper citation: RRID:Addgene_62219 Copy
Species: Synthetic
Genetic Insert: Luciferase
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Mammalian Expression, Luciferase, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25751063
Proper citation: RRID:Addgene_62170 Copy
Species: Synthetic
Genetic Insert: CreERT2
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25751063
Proper citation: RRID:Addgene_62174 Copy
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Mammalian Expression, RNAi, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25751063
Proper citation: RRID:Addgene_62181 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25751063
Proper citation: RRID:Addgene_62184 Copy
Species: Synthetic
Genetic Insert: tdTomato
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25751063
Proper citation: RRID:Addgene_62185 Copy
Species: Synthetic
Genetic Insert: ER-mRFP
Vector Backbone Description: Backbone Marker:clontech; Backbone Size:4000; Vector Backbone:clontech C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16617114
Comments: Red localization marker for mammalian endoplasmic reticulum.
Proper citation: RRID:Addgene_62236 Copy
Species: Synthetic
Genetic Insert: hamster BiP-mGFP-KDEL
Vector Backbone Description: Backbone Marker:clontech; Backbone Size:4000; Vector Backbone:clontech N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20410136
Comments: Avoid overexpression. Low expressing cells are best for imaging. High expression leads to protein aggregation in the nuclear envelope. See supplementary data for original paper.
Do NOT use this as an ER marker. BiP is a functional protein and significantly impacts the ER environment increasing stress resistance. Use ER-GFP if you simply want a marker for the ER when imaging cells.
Proper citation: RRID:Addgene_62231 Copy
Species: Synthetic
Genetic Insert: hamster BiP-mGFP-KDEL
Vector Backbone Description: Backbone Marker:clontech; Backbone Size:4000; Vector Backbone:clontech N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20410136
Comments: Avoid overexpression. Low expressing cells are best for imaging. High expression leads to protein aggregation in the nuclear envelope. See supplementary data for original paper.
This mutant can not bind client proteins.
Do NOT use this as an ER marker. BiP is a functional protein and significantly impacts the ER environment increasing stress resistance. Use ER-GFP if you simply want a marker for the ER when imaging cells.
Proper citation: RRID:Addgene_62232 Copy
Species: Synthetic
Genetic Insert: hamster BiP-mCherry-KDEL
Vector Backbone Description: Backbone Marker:clontech; Backbone Size:4000; Vector Backbone:clontech N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20410136
Comments: Avoid overexpression. Low expressing cells are best for imaging. High expression leads to protein aggregation in the nuclear envelope. See supplementary data for original paper.
Do NOT use this as an ER marker. BiP is a functional protein and significantly impacts the ER environment increasing stress resistance. Use ER-mCherry if you simply want a marker for the ER when imaging cells.
Proper citation: RRID:Addgene_62233 Copy
Species: Synthetic
Genetic Insert: Left CLYBL TALEN
Vector Backbone Description: Backbone Size:4681; Vector Backbone:pZT; Vector Types:TALEN; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25587899
Comments: pZT-C13/CLYBL TALENs were assembled using RVD monomers from Golden Gate TALEN kit 1.0 (TALEN Kit #1000000016) and a mammalian TALEN expression vector pZT
Proper citation: RRID:Addgene_62196 Copy
Species: Other
Genetic Insert: YFP
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25422271
Proper citation: RRID:Addgene_62245 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Cre recombinase under control of vasa promoter and terminator
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2700; Vector Backbone:pDONR P1-P4; Vector Types:Insect Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25869647
Proper citation: RRID:Addgene_62301 Copy
Species: Synthetic
Genetic Insert: tev 3x FLAG
Vector Backbone Description: Backbone Size:3519; Vector Backbone:pCRII blunt topo; Vector Types:yeast c-terminal tagging vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25378625
Proper citation: RRID:Addgene_62384 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5144; Vector Backbone:pDONR P4r-P3r; Vector Types:Insect Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25869647
Proper citation: RRID:Addgene_62289 Copy
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