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Species: Mus musculus
Genetic Insert: Ngn3 promoter
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript II KS; Vector Types:Mammalian Expression, Cre/Lox, mouse transgenic; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11973276
Comments: To generate the Ngn3 constructs, a 7.6 kb fragment containing Ngn3 (pGD15, contains the 6.5 kb upstream region of Ngn3) was isolated using Ngn3 cDNA to screen a mouse RPI-22 BAC library. The Ngn3-Cre construct was obtained by directly ligating a XbaI-BssHII (partial digestion) blunt-ended fragment from pGD15 to the pMC-Cre plasmid to generate pGD 84. The insert was released by NotI-SalI digestion and used for transgenic animal production.
See Author's Map and article for more information.
Proper citation: RRID:Addgene_15023 Copy
Species: Mus musculus
Genetic Insert: Pdx1 promoter
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript II KS; Vector Types:Mammalian Expression, Cre/Lox, mouse transgenic; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11973276
Comments: The CRE-ER(TM) protein requires tamoxifen (TM) to catalyze LoxP site-mediated excision.
For Pdx-1-Cre-ER(TM), the coding region of the Cre-ER(TM) cDNA was directly fused to the starting ATG of the PDX1 protein by PCR. Three primers (p5, 5'-ttgaaacaagtgcaggtgttcg-3'; p75, 5'-gttgcatcgaccggtaatgcaggcaaattttggtgtacggtcagtaaattggacatggtggcagccggcact-3'; and p71, 5'-gttgcatcgaccggtaatgca-3') were used. First, p5 and p75 were used to amplify a 400 base pair fragment from the Pdx1 genomic DNA. Then this fragment was used together with p5 and p71 and the Cre-ER(TM) plasmid to obtain a fragment that has the 5' end coding region directly fused to the Pdx1 promoter. This fragment was digested with AgeI and ligated to the XhoI (blunt-ended)-AgeI double-digested pBSCre-ER(TM) that contains the full-length Cre-ER-coding region to give pGD19. Then a 2.2 kb insert was released from pGD19 by SmaI-SpeI digestion and ligated to SmaI-NotI digested pKSpdx-1SalI, and a SpeI-NotI digested PCR fragment that contains the SV40 polyadenylation signal to give pGD35. The 8 kb insert was released by SalI-NotI digestion and was used for pronucleus injection.
See Author's Map and article for more information.
SalI/SmaI digest should give 6.7, 1.5, 4 kb bands.
Proper citation: RRID:Addgene_15022 Copy
Species: Mus musculus
Genetic Insert: Pdx1 promoter
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript II KS; Vector Types:Mammalian Expression, Cre/Lox, mouse transgenic; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11973276
Comments: To generate the Pdx1-Cre construct, a 5.5 kb SalI-SmaI fragment (blunt-ended) containing the Pdx1 promoter from pKSpdx-1SalI was ligated to the XbaI-digested (blunt-ended) pMC-Cre. The insert was released by SalI and NotI digestion.
See Author's Map for more information.
Proper citation: RRID:Addgene_15021 Copy
Species: Mus musculus
Genetic Insert: IFT88
Vector Backbone Description: Backbone Size:9800; Vector Backbone:pEF1α-IRES-EGFP (IRES-EGFP removed); Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_149697 Copy
Species: Mus musculus
Genetic Insert: CRX
Vector Backbone Description: Backbone Marker:Cepko Lab; Backbone Size:0; Vector Backbone:pLIA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9390562
Comments: For the CRX-EnR fusion construct, a 302 bp XmaI fragment (amino acids 7–108) containing the Crx homeobox was fused to the EnR domain. To make the CRX-EnR fusion, the XmaI Crx fragment, an EcoRI-XmaI linker encoding amino acids 2–6 with a NcoI site at the methionine of position 2, an XmaI-ClaI linker, and a ClaI-NotI (partial digestion) 930 bp fragment of the EnR were ligated into a shuttle vector, pSLAX21. The ClaI site inside this construct was designed so that the entire CRX-EnR fusion could be excised as a 1.3 kb ClaI fragment from pSLAX21/CRX-EnR. The ClaI fragment was ligated into pLIA.
Proper citation: RRID:Addgene_15155 Copy
Species: Mus musculus
Genetic Insert: Rax
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript KS+; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9096350
Comments: Rax cDNA
Proper citation: RRID:Addgene_15138 Copy
Species: Mus musculus
Genetic Insert: Notch1 (C-term)
Vector Backbone Description: Backbone Marker:Cepko Lab; Backbone Size:0; Vector Backbone:pLIA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9006984
Comments: mNIC encodes the cytoplasmic domain of mouse Notch 1 with a myc tag at the N terminus.
Proper citation: RRID:Addgene_15131 Copy
Species: Mus musculus
Genetic Insert: CRX
Vector Backbone Description: Backbone Marker:Cepko Lab; Backbone Size:6500; Vector Backbone:pLIA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9390562
Comments: An EcoRV-HincII Crx cDNA fragment containing the entire Crx coding region was subcloned into the SnaBI site of the pLIA retrovirus vector.
Proper citation: RRID:Addgene_15128 Copy
Species: Mus musculus
Genetic Insert: CRX
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript KS+; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9390562
Comments: CRX cDNA
Proper citation: RRID:Addgene_15127 Copy
Species: Mus musculus
Genetic Insert: beta2-YFP-M
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5500; Vector Backbone:pCI-Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14684858
Comments: YFP with an upstream HA epitope tag was inserted into PpuMI restriction site of the (M3-M4) loop at residue 381 (beta2-YFP-M).
Proper citation: RRID:Addgene_15107 Copy
Species: Mus musculus
Genetic Insert: p27
Vector Backbone Description: Backbone Marker:Cepko Lab; Backbone Size:0; Vector Backbone:pLIA; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11404411
Proper citation: RRID:Addgene_15191 Copy
Species: Mus musculus
Genetic Insert: PIAS3
Vector Backbone Description: Backbone Size:3800; Vector Backbone:pCMV-Flag; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9388184
Comments: Please note that Addgene's sequencing results identified a V580I difference when compared to GenBank reference sequence AAB88902.2. The depositing laboratory has confirmed that plasmid this is exact construct used in the associated publication.
Proper citation: RRID:Addgene_15207 Copy
Species: Mus musculus
Genetic Insert: Chx10 3kb AP reporter
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15893976
Comments: The 3Kb-AP reporter was constructed using a
genomic BamHI–SacII fragment isolated from a Chx10
BAC (Rowan and Cepko, 2004) and subcloned into pBluescript
(Stratagene). An SV40 intervening sequence (IVS)
was isolated from pNASSbeta (Clontech) digested with EcoRI
and blunt cloned into the 3' SacII restriction site. The full length alkaline phosphatase (AP) reporter construct was
isolated from the SV40all plasmid (gift of S. Dymecki).
Proper citation: RRID:Addgene_15205 Copy
Species: Mus musculus
Genetic Insert: Neuroligin 1 with insert A
Vector Backbone Description: Backbone Size:4842; Vector Backbone:pCAAGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16846852
Proper citation: RRID:Addgene_15227 Copy
Species: Mus musculus
Genetic Insert: MED9
Vector Backbone Description: Backbone Marker:Invitrogen, modified at Conaway lab; Backbone Size:5600; Vector Backbone:pcDNA3.1 N-FLAG/Hyg; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14638676
Proper citation: RRID:Addgene_15407 Copy
Species: Mus musculus
Genetic Insert: sgRNA and BAIT targeting murine B2M locus
Vector Backbone Description: Backbone Marker:Derived from pX458 (Zhang lab); Backbone Size:9500; Vector Backbone:pQCi; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33616439
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.03.25.008276v4 for bioRxiv preprint.
Proper citation: RRID:Addgene_154091 Copy
Species: Mus musculus
Genetic Insert: MED10
Vector Backbone Description: Backbone Marker:Invitrogen, modified at Conaway lab; Backbone Size:5600; Vector Backbone:pcDNA3.1 N-FLAG/Hyg; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12584197
Proper citation: RRID:Addgene_15417 Copy
Species: Mus musculus
Genetic Insert: CHMP3
Vector Backbone Description: Backbone Marker:#11154; Backbone Size:4311; Vector Backbone:pEF-GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34597582
Proper citation: RRID:Addgene_154177 Copy
Species: Mus musculus
Genetic Insert: retroCHMP3
Vector Backbone Description: Backbone Marker:#11154; Backbone Size:4311; Vector Backbone:pEF-GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34597582
Proper citation: RRID:Addgene_154178 Copy
Species: Mus musculus
Genetic Insert: CHMP3
Vector Backbone Description: Backbone Marker:EvNO00601609; Backbone Size:5422; Vector Backbone:pCMV(WT); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34597582
Proper citation: RRID:Addgene_154175 Copy
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