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Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5488; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: Please note that the hygromycin cassette in this plasmid is non-functional. See provided protocol for high-throughput, ligation-independent cloning using this plasmid.
Proper citation: RRID:Addgene_40728 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20733051
Comments: Venus YFP-3xFlag was inserted via BstXI-XhoI sites (to create pcDNA5/FRT/TO-Venus-Flag, Addgene Plasmid# 40998).
The plasmid was then converted to a Gateway destination vector by inserting Gateway cassette C1, containing recombination sites attR1 and attR2, CmR, and ccdB, into the Afl II site (blunt ended).
This plasmid can be used to place genes into the pcDNA5/FRT/TO-Venus-Flag construct by Gateway reaction, to make tetracycline-inducible stable cell lines.
For subcloning, the following restriction sites are present:
Between the CMV promoter and the Gateway cassette: PmeI (non-unique)
Between the Gateway cassette and Venus: HindIII, Asp718I, KpnI, BamHI (non-unique, also in GW casette), and BstXI
Between Venus and BGHpA: XhoI, Eco0109I, ApaI, and PmeI (non-unique)
Proper citation: RRID:Addgene_40999 Copy
Vector Backbone Description: Backbone Size:7522; Vector Backbone:pKW32; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18064608
Comments: pWK32 Gateway-compatible Destination vector. Combine with an Entry clone containing your gene of interest with the Gateway LR reaction to generate an expression clone containing a hemoglobin tag.
Proper citation: RRID:Addgene_18669 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7694; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18064608
Comments: pcDNA3 Gateway-compatible Destination vector. Combine with an Entry clone containing your gene of interest with the Gateway LR reaction to generate a mammalian expression clone.
Proper citation: RRID:Addgene_18672 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:11282; Vector Backbone:pPIC3.5K; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18064608
Comments: pPIC3.5K Gateway-compatible Destination vector. Combine with an Entry clone containing your gene of interest with the Gateway LR reaction for integration of gene of interest into Pichia.
Proper citation: RRID:Addgene_18707 Copy
Vector Backbone Description: Backbone Size:9313; Vector Backbone:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:19657394
Proper citation: RRID:Addgene_19065 Copy
Vector Backbone Description: Backbone Size:9778; Vector Backbone:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:19657394
Proper citation: RRID:Addgene_19067 Copy
Vector Backbone Description: Backbone Size:10301; Vector Backbone:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:19657394
Comments: There are several mismatches between Addgene's quality control sequence and depositor's reference sequence. The mismatches are in non-coding regions and do not affect the features.
Proper citation: RRID:Addgene_19066 Copy
Vector Backbone Description: Backbone Size:9599; Vector Backbone:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:19657394
Proper citation: RRID:Addgene_19068 Copy
Species: Caenorhabditis elegans
Genetic Insert: pDESTttTi5605[R4-R3]
Vector Backbone Description: Backbone Size:2354; Vector Backbone:N/A; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18953339
Comments: Invitrogen Gateway 3-fragment compatible destination vector. Contains C.Briggsae unc-119 rescue fragment. Also contains genomic regions flanking ttTi5605 Mos1 insertion to generate MosSCI inserts at this locus.
Please note that due to a bug in the Addgene map algorithm, the attR sites are depicted as attR1 and attR2, when in fact they are attR3 and attR4. Please refer to the author's map for an accurate depiction of these sites.
Proper citation: RRID:Addgene_19329 Copy
Species: Caenorhabditis elegans
Genetic Insert: Gateway(R1-R2)<4xNLS-GFP::let-858(3’)-(Cbr)unc-119(+)<4xNLS-tdimer2(12)::let-858(3’)
Vector Backbone Description: Backbone Size:0; Vector Backbone:pGC91; Vector Types:Worm Expression, FLP/FRT, Destination vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18723890
Comments: Sequence mismatches between Addgene sequence and depositor sequence do not affect function.
Proper citation: RRID:Addgene_19630 Copy
Species: Caenorhabditis elegans
Genetic Insert: Gateway(R1-R2)
Defining Citation: PMID:18723890
Comments: Sequence mismatches between Addgene sequence and depositor sequence do not affect function.
Proper citation: RRID:Addgene_19632 Copy
Species: Caenorhabditis elegans
Genetic Insert: Gateway(R1-R2)
Defining Citation: PMID:18723890
Proper citation: RRID:Addgene_19631 Copy
Species: Caenorhabditis elegans
Genetic Insert: Gateway(R1-R2)
Defining Citation: PMID:18723890
Proper citation: RRID:Addgene_19634 Copy
Species: Caenorhabditis elegans
Genetic Insert: Gateway(R1-R2)<4xNLS-GFP::let-858(3’)-(Cbr)unc-119(+)
Defining Citation: PMID:18723890
Proper citation: RRID:Addgene_19633 Copy
Species: Caenorhabditis elegans
Genetic Insert: Gateway(R1-R2)<4xNLS-tdimer2(12)::let-858(3’)
Defining Citation: PMID:18723890
Proper citation: RRID:Addgene_19636 Copy
Species: Caenorhabditis elegans
Genetic Insert: Gateway(R4-R3)
Defining Citation: PMID:18723890
Comments: Sequence mismatches between Addgene sequence and depositor sequence do not affect function.
Proper citation: RRID:Addgene_19638 Copy
Species: Caenorhabditis elegans
Genetic Insert: Gateway(attR1-attR2)::FLP::let-858(3’)-(Cbr)unc-119(+)
Vector Backbone Description: Backbone Size:0; Vector Backbone:pGC31; Vector Types:Worm Expression, FLP/FRT, Destination vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18723890
Proper citation: RRID:Addgene_19616 Copy
Species: Caenorhabditis elegans
Genetic Insert: Gateway(R1-R2)<4xNLS-GFP::let-858(3’)-(Cbr)unc-119(+)
Defining Citation: PMID:18723890
Proper citation: RRID:Addgene_19629 Copy
Species: Caenorhabditis elegans
Genetic Insert: Gateway(R1-R2)<4xNLS-GFP::let-858(3’)-(Cbr)unc-119(+)<1xNLS-lacZ::unc-54(3’)
Vector Backbone Description: Backbone Size:0; Vector Backbone:pGC91; Vector Types:Worm Expression, FLP/FRT, destination vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18723890
Comments: Sequence mismatches between Addgene sequence and depositor sequence do not affect function.
Proper citation: RRID:Addgene_19628 Copy
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