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Species: Mus musculus
Genetic Insert: Lpl in situ probe
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pCRII-Topo; Vector Types:in situ; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19793993
Comments: The Lpl fragment was amplified by RT-PCR using the following primer pair:
TGCTGTGCAAAGAGAAGAGC
CGGACACAAAGTTAGCACCA
For in vitro transcription, use the NotI restriction digest and Sp6 promoter for sense probe generation and the HindIII restriction digest and T7 promoter for antisense probe generation.
Please note that Addgene's sequencing results match bp# 3249-3900 of NM_008509.2, not bp# 3169-3826 as indicated on plasmid map. The plasmid functions as described in the associated publication.
Proper citation: RRID:Addgene_45630 Copy
Species: Mus musculus
Genetic Insert: Nectin-3 in situ probe
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pCRII-Topo; Vector Types:in situ; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19793993
Comments: The Nectin-3 fragment was amplified by RT-PCR using the following primer pair:
AAACAACCTGATCCGCAAAG
CAGTGAAAACTGTAAAGCAGCTC
For in vitro transcription, use the BamHI restriction digest and T7 promoter for sense probe generation and the NotI restriction digest and Sp6 promoter for antisense probe generation.
Please note that Addgene's sequencing results match bp# 1626-2039 of NM_021495, not bp# 1624-2036 as indicated on plasmid map. The plasmid functions as described in the associated publication.
Proper citation: RRID:Addgene_45633 Copy
Species: Mus musculus
Genetic Insert: Ptn in situ probe
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pCRII-Topo; Vector Types:in situ; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19793993
Comments: The Ptn fragment was amplified by RT-PCR using the following primer pair:
GCCTACCCGTCCAAATATCC
GCCAGTTCTGGTCTTCAAGG
For in vitro transcription, use the NotI restriction digest and SP6 promoter for sense probe generation and the HindIII restriction digest and T7 promoter for antisense probe generation.
Please note that Addgene's sequencing results match bp# 1241-1830 of NM_008973, not bp# 1238-1827 as indicated on plasmid map. The plasmid functions as described in the associated publication.
Proper citation: RRID:Addgene_45632 Copy
Species: Rattus norvegicus
Genetic Insert: AT1AR-TSTS/A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5000; Vector Backbone:pcDNA3.1/Zeo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15355986
Comments: Mutant AT1A receptors were generated by mutagenesis PCR using pcDNA3.1-HA-AT1A receptor (provided by M. G. Caron, Duke University) as a template and a QuikChange multisite-directed mutagenesis kit (Stratagene) according to the manufacturer’s instructions. The primer for the TSTS/A receptor was:
5' p-CTCAAGCCTGTCTGCGAAAATGGCCGCTTGCTTACCGGCCTTC 3'.
N4D mutation introduced to prevent glycosylation at this consensus site and steric hindrance of antibody binding.
Proper citation: RRID:Addgene_45634 Copy
Species: Mus musculus
Genetic Insert: Gpr88 in situ probe
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pCRII-Topo; Vector Types:in situ; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19793993
Comments: The Gpr88 fragment was amplified by RT-PCR using the following primer pair:
CAAATGAAACCAATGGTCAGG
TATCTGTTTCCCGTGTCTCC
For in vitro transcription, use the HindIII restriction digest and T7 promoter for sense probe generation and the NotI restriction digest and Sp6 promoter for antisense probe generation.
Please note that Addgene's sequencing results match bp# 2742-3255 of AB042408.1 or bp# 2772-3285 of NM_022427, not bp# 2742-3255 of NM_022427 as indicated on plasmid map. The plasmid functions as described in the associated publication.
Proper citation: RRID:Addgene_45628 Copy
Species: Mus musculus
Genetic Insert: Foxp2 in situ probe
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pCRII-Topo; Vector Types:in situ; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16157277
Comments: The Fox2p fragment was amplified by RT-PCR using the following primer pair:
CAGTGTGGACTGTGGACGAA
TTCCAGGTCCTCAGATAAAGG
For in vitro transcription, use the HindIII restriction digest and T7 promoter for sense probe generation and the NotI restriction digest and SP6 promoter for antisense probe generation.
Please note that Addgene's sequencing results match bp#1709-2142 of AF339106, not bp# 1707-2142 as indicated on plasmid map. The plasmid functions as described in the associated publication.
Proper citation: RRID:Addgene_45620 Copy
Species: Mus musculus
Genetic Insert: Tcrb-V13 in situ probe
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pCRII-Topo; Vector Types:in situ; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16157277
Comments: The Tcrb-V13 fragment was amplified by RT-PCR using the following primer pair:
CTGAGCTGGTGGGTGAATG
AAGGTGTCAACGAGGAAGGA
For in vitro transcription, use the NotI restriction digest and Sp6 promoter for sense probe generation and the HindIII restriction digest and T7 promoter for antisense probe generation.
Please note that Addgene's sequencing results match bp# 533-1058 of X67128, not bp# 532-1059 as indicated on plasmid map. The plasmid functions as described in the associated publication.
Proper citation: RRID:Addgene_45622 Copy
Species: Mus musculus
Genetic Insert: Plexin D1 in situ probe
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pCRII-Topo; Vector Types:in situ; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16157277
Comments: The Plexin D1 fragment was amplified by RT-PCR using the following primer pair:
CAGGAAATGAACGCACACC
TGAGGGACACAGACAACTGC;
For in vitro transcription, use the HindIII restriction digest and T7 promoter for sense probe generation and the NotI restriction digest and SP6 promoter for antisense probe generation.
Proper citation: RRID:Addgene_45621 Copy
Species: Mus musculus
Genetic Insert: Cux2 in situ probe
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pCRII-Topo; Vector Types:in situ; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16157277
Comments: The Cux2 fragment was amplified by RT-PCR using the following primer pair:
TCAGTCAACAGCTCCATTCG
GACAGCGAGAAAGTCCTTGG
For in vitro transcription, use the HindIII restriction digest and T7 promoter for sense probe generation and the NotI restriction digest and SP6 promoter for antisense probe generation.
Proper citation: RRID:Addgene_45623 Copy
Species: Nostoc punctiforme
Genetic Insert: GFPN-NpuDnaE_intein-GFP_C
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:4597; Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23974115
Proper citation: RRID:Addgene_45615 Copy
Species: (Methanocaldococcus jannaschii DSM 2661)
Genetic Insert: MjaTFIIBC53
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4597; Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23974115
Comments: There are 2 mismatches between Addgene's quality control and depositor's reference sequence. These changes are in backbone region and should not affect function.
Proper citation: RRID:Addgene_45616 Copy
Genetic Insert: co I-Sce I
Vector Backbone Description: Backbone Size:5071; Vector Backbone:pCVL; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24121685
Proper citation: RRID:Addgene_45574 Copy
Species: Homo sapiens
Genetic Insert: eGFP with I-Sce I TS
Vector Backbone Description: Backbone Size:4483; Vector Backbone:pCVL; Vector Types:Mammalian Expression, Bacterial Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24121685
Proper citation: RRID:Addgene_45575 Copy
Genetic Insert: coY2 Ani
Vector Backbone Description: Backbone Size:5071; Vector Backbone:pCVL; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24121685
Proper citation: RRID:Addgene_45578 Copy
Species: Homo sapiens
Genetic Insert: FGFR2 IIIc
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pBabe puro Gateway; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23786770
Proper citation: RRID:Addgene_45699 Copy
Species: Pyrococcus horikoshii
Genetic Insert: PhoRadAC46
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:4597; Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23974115
Comments: There are 2 mismatches between Addgene's quality control and depositor's reference sequence. These changes are in backbone region and should not affect function.
Proper citation: RRID:Addgene_45610 Copy
Species: Homo sapiens
Genetic Insert: FGFR2 IIIb
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pBabe puro Gateway; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23786770
Proper citation: RRID:Addgene_45698 Copy
Genetic Insert: coY2 Ani K227M
Vector Backbone Description: Backbone Size:5071; Vector Backbone:pCVL; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24121685
Proper citation: RRID:Addgene_45579 Copy
Species: nostoc punctiforme
Genetic Insert: NpuDnaBC39
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4597; Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23974115
Comments: There are 2 mismatches between Addgene's quality control and depositor's reference sequence. These changes are in backbone region and should not affect function.
Proper citation: RRID:Addgene_45612 Copy
Species: Homo sapiens
Genetic Insert: TRIM28
Vector Backbone Description: Backbone Marker:Amersham GE Healthcare; Backbone Size:5000; Vector Backbone:pGEX-5X; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21940674
Comments: Full-length human TRIM28 was purchased from Origene and subcloned into vector pKH3 to generate pKH3-Trim28 (Addgene plasmid #45569). A full length TRIM28 DNA fragment was then generated from pKH3-TRIM28 by PCR and cloned into pGEX-5X.
Proper citation: RRID:Addgene_45570 Copy
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