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Vector Backbone Description: Backbone Marker:Messing Lab; Backbone Size:2686; Vector Backbone:pUC6, Addgene plasmid #49793; Vector Types:cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:6323249
Comments: Identical to pUC18 (Addgene plasmid #50004) except that the multiple cloning sites (MCS) are arranged in opposite orientation.
Proper citation: RRID:Addgene_50005 Copy
Species: Other
Genetic Insert: promoter nos (A. tumefaciens)
Vector Backbone Description: Vector Backbone:pICH41233; Vector Types:plant expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24933124
Comments: insert can be released with BsaI
Proper citation: RRID:Addgene_50255 Copy
Species: Homo sapiens
Genetic Insert: ring finger protein 31
Vector Backbone Description: Backbone Marker:Agilent (Stratagene); Backbone Size:5191; Vector Backbone:pCMV-3Tag-8; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24060851
Proper citation: RRID:Addgene_50015 Copy
Species: Homo sapiens
Genetic Insert: SHANK-associated RH domain interactor
Vector Backbone Description: Backbone Marker:Agilent (Stratagene); Backbone Size:5171; Vector Backbone:pCMV-3Tag-8; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24060851
Proper citation: RRID:Addgene_50014 Copy
Species: Bacteriophage T7
Genetic Insert: T7 RNAP*
Vector Backbone Description: Vector Backbone:pIncW; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24169405
Comments: CDS of the T7 RNAP starts with the GTG at the beginning of the sequence.
Proper citation: RRID:Addgene_49990 Copy
Species: Other
Genetic Insert: promoter (0.4 kb ) + 5'UTR, 35s (Cauliflower Mosaic Virus)
Vector Backbone Description: Vector Backbone:pICSL01008; Vector Types:plant expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24933124
Comments: insert can be released with BsaI
Proper citation: RRID:Addgene_50266 Copy
Species: Other
Genetic Insert: promoter (0.4 kb), 35s (Cauliflower Mosaic Virus) + 5'UTR, omega (Tobacco Mosaic Virus)
Vector Backbone Description: Vector Backbone:pICH41295; Vector Types:Other, plant expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24933124
Comments: insert can be released with BsaI
Proper citation: RRID:Addgene_50268 Copy
Species: Other
Genetic Insert: C terminal HA tag (6x Human influenza hemagglutinin)
Vector Backbone Description: Vector Backbone:PAGM1301; Vector Types:plant expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24933124
Comments: insert can be released with BsaI
Proper citation: RRID:Addgene_50309 Copy
Species: HPV
Genetic Insert: HPV 16 E6no*
Vector Backbone Description: Backbone Size:6550; Vector Backbone:pNCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22553330
Comments: *The V42L mutation prevents the internal splicing of the DNA so only the "no *" (unspliced form) of 18E6 is produced.
Proper citation: RRID:Addgene_47706 Copy
Species: Synthetic
Genetic Insert: Codon-optimised MSP1
Vector Backbone Description: Backbone Marker:Yves Durocher, NRC; Backbone Size:6633; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24043421
Proper citation: RRID:Addgene_47709 Copy
Species: C. reinhardtii
Genetic Insert: hChR2 (H134R)
Vector Backbone Description: Backbone Marker:Karl Deisseroth; Backbone Size:5637; Vector Backbone:pAAV EF1a DIO; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28380331
Proper citation: RRID:Addgene_47633 Copy
Genetic Insert: eNpHR3.0
Vector Backbone Description: Backbone Marker:Karl Deisseroth; Backbone Size:5637; Vector Backbone:pAAV EF1a DIO; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28380331
Proper citation: RRID:Addgene_47631 Copy
Species: Discosoma sp
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:Karl Deisseroth; Backbone Size:5637; Vector Backbone:pAAV EF1a DIO; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28380331
Comments: This vector has a different 5' cloning junction when compared to Karl Deisseroth's Plasmid 20299.
Proper citation: RRID:Addgene_47636 Copy
Species: Homo sapiens
Genetic Insert: PMCA4bct120
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: x/b splice variant of human PMCA4
Proper citation: RRID:Addgene_47593 Copy
Species: Homo sapiens
Genetic Insert: PMCA4x/b
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:12624087
Comments: HPMCA4x/b coding sequence starts with amino acid 2 (Thr) at pos 1354.
This plasmid carries two missense mutations that cause K442R and P663L in the amino acid sequence of hPMCA4b. The depositing lab did not notice any functional consequences in the assays they performed. However, these were mostly concerned with membrane localization and did not include any enzymatic functional studies. Thus, it is not know whether (and how) these two mutations affect the calcium-pumping enzymatic function of the encoded GFP-hPMCA4b protein.
Proper citation: RRID:Addgene_47589 Copy
Species: Homo sapiens
Genetic Insert: PMCA2w/b
Vector Backbone Description: Backbone Marker:Clontech; modified as described in Rogers et al JBC 2003; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:12624087
Comments: w/b splice variant of human PMCA2. PMCA2w/b inserted as SacII-XbaI fragment into multiple cloning site but is also flanked by NotI sites. PMCA2 start: 1396; stop: 5125, w-splice: 2303
Proper citation: RRID:Addgene_47586 Copy
Species: Rhodopseudomonas palustris
Genetic Insert: Infrared fluorescent protein
Vector Backbone Description: Backbone Marker:Karl Deisseroth; Backbone Size:5637; Vector Backbone:pAAV EF1a DIO; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28380331
Proper citation: RRID:Addgene_47626 Copy
Species: Homo sapiens
Genetic Insert: PMCA2z/b
Vector Backbone Description: Backbone Marker:Penniston Lab; Backbone Size:5145; Vector Backbone:pMM2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12624087
Comments: z/b splice variant of human PMCA2. PMCA2 start: 1104; stop: 4700, z-splice: 2010/11; b-splice: 4389-4700
Proper citation: RRID:Addgene_47581 Copy
Species: Homo sapiens
Genetic Insert: pMCA2z/b
Vector Backbone Description: Backbone Marker:Clontech; modified as described in Rogers et al JBC 2001; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:12624087
Comments: z/b splice variant of human PMCA2
Proper citation: RRID:Addgene_47584 Copy
Species: Mus musculus
Genetic Insert: Notch3 Intracellular domain
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pCMX; Vector Types:Mammalian Expression, in vitro transcription and translation; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8951795
Proper citation: RRID:Addgene_47618 Copy
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