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Species: Caenorhabditis elegans
Genetic Insert: vhhGFP4-ZIF-1
Vector Backbone Description: Backbone Marker:Christian Frøkjær-Jensen (Erik Jorgensen lab); Backbone Size:7328; Vector Backbone:pCFJ151; Vector Types:Targeting vector for Mos1 transposon mediated single copy transgene insertion; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28619826
Proper citation: RRID:Addgene_89357 Copy
Species: Caenorhabditis elegans
Genetic Insert: ChR2(gf)
Vector Backbone Description: Backbone Size:3684; Vector Backbone:pPD96.52; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22081161
Comments: This plasmid contains 1-51 of Uncharacterized protein CELE_F32A7.6 (NP_493594.1). The depositor states that this should not adversely affect plasmid function.
Proper citation: RRID:Addgene_78196 Copy
Species: Caenorhabditis elegans
Genetic Insert: ChR2(gf)
Vector Backbone Description: Backbone Size:3684; Vector Backbone:pPD96.52; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22081161
Comments: This plasmid contains 1-51 of Uncharacterized protein CELE_F32A7.6 (NP_493594.1). The depositor states that this should not adversely affect plasmid function.
Proper citation: RRID:Addgene_78195 Copy
Species: Caenorhabditis elegans
Genetic Insert: cha-1
Vector Backbone Description: Vector Backbone:pPD114.108; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22081161
Proper citation: RRID:Addgene_78192 Copy
Species: Caenorhabditis elegans
Genetic Insert: cha-1
Vector Backbone Description: Vector Backbone:pPD114.108; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22081161
Proper citation: RRID:Addgene_78194 Copy
Species: Caenorhabditis elegans
Genetic Insert: rpy-1
Vector Backbone Description: Vector Backbone:pPD95.75; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19158078
Proper citation: RRID:Addgene_78185 Copy
Species: Caenorhabditis elegans
Genetic Insert: hsp-16.1
Vector Backbone Description: Vector Backbone:pPD95.77; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23229554
Proper citation: RRID:Addgene_78190 Copy
Species: Caenorhabditis elegans
Genetic Insert: tomm20::miniSOG
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:5725; Vector Backbone:pBSK; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29360035
Proper citation: RRID:Addgene_179577 Copy
Species: Caenorhabditis elegans
Genetic Insert: tomm20::miniSOG
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:7805; Vector Backbone:pBSK; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29360035
Proper citation: RRID:Addgene_179574 Copy
Species: Caenorhabditis elegans
Genetic Insert: tomm20::miniSOG
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:6791; Vector Backbone:pBSK; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29360035
Proper citation: RRID:Addgene_179630 Copy
Species: Caenorhabditis elegans
Genetic Insert: tomm20::miniSOG
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:8030; Vector Backbone:pBSK; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29360035
Proper citation: RRID:Addgene_179542 Copy
Species: Caenorhabditis elegans
Genetic Insert: tomm20::miniSOG
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:6180; Vector Backbone:pBSK; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29360035
Proper citation: RRID:Addgene_179541 Copy
Species: Caenorhabditis elegans
Genetic Insert: NLF-1
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:7378; Vector Backbone:pBSK; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23522043
Proper citation: RRID:Addgene_179780 Copy
Species: Caenorhabditis elegans
Genetic Insert: Mex-5
Vector Backbone Description: Backbone Marker:Cheeseman lab; Backbone Size:16397; Vector Backbone:pIC26; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21925318
Comments: MEX-5 transgenes driven by the pie-1 promoter and pie-1 3'UTR were constructed by cloning the mex-5 cDNA as a SpeI fragment downstream of a Dendra2/TEV/S-peptide tag cloned into pIC26 LAP tag (Cheeseman et al., 2004).
Alternate plasmid name: pIC26 + MEX-5 (Dendra + MEX-5 ORF)
Proper citation: RRID:Addgene_40077 Copy
Species: Caenorhabditis elegans
Genetic Insert: Mex-5
Vector Backbone Description: Backbone Marker:New England Biolabs (Modified); Backbone Size:6700; Vector Backbone:pMal c2X (GW); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21925318
Comments: Alternate plasmid name: pMAL c2X (GW) + MEX-5
Proper citation: RRID:Addgene_40074 Copy
Species: Caenorhabditis elegans
Genetic Insert: Mex-5 (S404A) (RNAi resistant)
Vector Backbone Description: Backbone Marker:Addgene plasmid 17247; Backbone Size:6351; Vector Backbone:pCG150; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21925318
Comments: Dendra::MEX-5 constructs were constructed as follows. A 4.4 kb mex-5 promoter fragment based on Tenlen et al. (2008) was cloned into pDONRP4P1R (Invitrogen). Dendra2/TEV/S-peptide (Gallo et al., 2010) was cloned into pDONR201. A MEX-5 genomic fragment from the start ATG through 648 bp of 3'UTR was cloned into pDONRP2RP3. Exon 2 of mex-5 was recoded to be RNAi-resistant (Gen-Script) so as to allow depletion of endogenous MEX-5/6 without depletion of the transgene. These constructs were assembled into pCG150 using three-way Gateway system (LR reaction) (Invitrogen) (Merritt et al., 2008). Mutations were made by recombinant PCR and all inserts were sequenced verified.
Alternate plasmid name: mex-5 prom::Dendra2/TEV/Speptide::MEX-5RR(S404A) - mex-5 3'UTR
Proper citation: RRID:Addgene_40192 Copy
Species: Caenorhabditis elegans
Genetic Insert: Mex-5 (RNAi resistant)
Vector Backbone Description: Backbone Marker:Addgene plasmid 17247; Backbone Size:6351; Vector Backbone:pCG150; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21925318
Comments: Dendra::MEX-5 constructs were constructed as follows. A 4.4 kb mex-5 promoter fragment based on Tenlen et al. (2008) was cloned into pDONRP4P1R (Invitrogen). Dendra2/TEV/S-peptide (Gallo et al., 2010) was cloned into pDONR201. A MEX-5 genomic fragment from the start ATG through 648 bp of 3'UTR was cloned into pDONRP2RP3. Exon 2 of mex-5 was recoded to be RNAi-resistant (Gen-Script) so as to allow depletion of endogenous MEX-5/6 without depletion of the transgene. These constructs were assembled into pCG150 using three-way Gateway system (LR reaction) (Invitrogen) (Merritt et al., 2008). Mutations were made by recombinant PCR and all inserts were sequenced verified.
Alternate plasmid name: mex-5 prom::Dendra2/TEV/Speptide::MEX-5RR - mex-5 3'UTR
Proper citation: RRID:Addgene_40191 Copy
Species: Caenorhabditis elegans
Genetic Insert: Mex-5 (S404A)
Vector Backbone Description: Backbone Marker:Cheeseman lab; Backbone Size:16397; Vector Backbone:pIC26; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21925318
Comments: MEX-5 transgenes driven by the pie-1 promoter and pie-1 3'UTR were constructed by cloning the mex-5 cDNA as a SpeI fragment downstream of a Dendra2/TEV/S-peptide tag cloned into pIC26 LAP tag (Cheeseman et al., 2004).
Alternate plasmid name: pie-1 prom – Dendra2/TEV/Speptide/MEX-5(S404A) - pie-1 3'UTR
Proper citation: RRID:Addgene_40198 Copy
Species: Caenorhabditis elegans
Genetic Insert: Mex-5 (S458A) (RNAi resistant)
Vector Backbone Description: Backbone Marker:Addgene plasmid 17247; Backbone Size:6351; Vector Backbone:pCG150; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21925318
Comments: Dendra::MEX-5 constructs were constructed as follows. A 4.4 kb mex-5 promoter fragment based on Tenlen et al. (2008) was cloned into pDONRP4P1R (Invitrogen). Dendra2/TEV/S-peptide (Gallo et al., 2010) was cloned into pDONR201. A MEX-5 genomic fragment from the start ATG through 648 bp of 3'UTR was cloned into pDONRP2RP3. Exon 2 of mex-5 was recoded to be RNAi-resistant (Gen-Script) so as to allow depletion of endogenous MEX-5/6 without depletion of the transgene. These constructs were assembled into pCG150 using three-way Gateway system (LR reaction) (Invitrogen) (Merritt et al., 2008). Mutations were made by recombinant PCR and all inserts were sequenced verified.
Alternate plasmid name: mex-5 prom::Dendra2/TEV/Speptide::MEX-5RR - mex-5 3'UTR
Proper citation: RRID:Addgene_40196 Copy
Species: Caenorhabditis elegans
Genetic Insert: Mex-5 (aa1-355) (RNAi resistant)
Vector Backbone Description: Backbone Marker:Addgene plasmid 17247; Backbone Size:6351; Vector Backbone:pCG150; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21925318
Comments: Dendra::MEX-5 constructs were constructed as follows. A 4.4 kb mex-5 promoter fragment based on Tenlen et al. (2008) was cloned into pDONRP4P1R (Invitrogen). Dendra2/TEV/S-peptide (Gallo et al., 2010) was cloned into pDONR201. A MEX-5 genomic fragment from the start ATG through 648 bp of 3'UTR was cloned into pDONRP2RP3. Exon 2 of mex-5 was recoded to be RNAi-resistant (Gen-Script) so as to allow depletion of endogenous MEX-5/6 without depletion of the transgene. These constructs were assembled into pCG150 using three-way Gateway system (LR reaction) (Invitrogen) (Merritt et al., 2008). Mutations were made by recombinant PCR
and all inserts were sequenced verified.
Alternate plasmid name: mex-5 prom::Dendra2/TEV/Speptide::MEX-5RR - mex-5 3'UTR
Proper citation: RRID:Addgene_40195 Copy
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