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Genetic Insert: mKO1
Vector Backbone Description: Vector Backbone:pFA6a-link; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: This vector is derived from the sequences published in “Optimized cassettes for fluorescent protein tagging in Saccharomyces cerevisiae.” Sheff MA, Thorn KS. Yeast. 2004 Jun;21(8):661-70.
Proper citation: RRID:Addgene_44642 Copy
Genetic Insert: yEmCitrine
Vector Backbone Description: Vector Backbone:pFA6a-link; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: This vector is derived from the sequences published in “Optimized cassettes for fluorescent protein tagging in Saccharomyces cerevisiae.” Sheff MA, Thorn KS. Yeast. 2004 Jun;21(8):661-70.
Proper citation: RRID:Addgene_44645 Copy
Genetic Insert: mDsRed
Vector Backbone Description: Vector Backbone:pFA6a-link; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: This vector is derived from the sequences published in “Optimized cassettes for fluorescent protein tagging in Saccharomyces cerevisiae.” Sheff MA, Thorn KS. Yeast. 2004 Jun;21(8):661-70.
Proper citation: RRID:Addgene_44639 Copy
Species: Mus musculus
Genetic Insert: BRCA1
Vector Backbone Description: Backbone Marker:Robert Weinberg (Addgene plasmid #8453); Backbone Size:7032; Vector Backbone:pLKO.1 puro; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23389450
Comments: Observed knockdown = 85%
Proper citation: RRID:Addgene_44595 Copy
Species: Homo sapiens
Genetic Insert: EF1a-GFP:2A:CHER(HA)
Vector Backbone Description: Backbone Marker:LJ Chang; Backbone Size:8680; Vector Backbone:pTY; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21647387
Comments: GFP-2A-mCherH was created using a three-step PCR strategy described by Symczak et al [46] that included the addition of a GSG linker sequence upstream of the PTV1 2A peptide sequence.
mCherry was tagged with HA to enable detection using HA antibody.
Proper citation: RRID:Addgene_44597 Copy
Species: Mus musculus
Genetic Insert: Gfi1
Vector Backbone Description: Backbone Marker:Warren Pear lab; Backbone Size:6500; Vector Backbone:NGFR (Addgene plasmid 27489); Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23444353
Proper citation: RRID:Addgene_44630 Copy
Species: Mus musculus
Genetic Insert: IRBP156-CHER
Vector Backbone Description: Backbone Marker:LJ Chang; Backbone Size:8701; Vector Backbone:pTY; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20517486
Proper citation: RRID:Addgene_44593 Copy
Species: Synthetic
Genetic Insert: Citrine-PTS1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:10404; Vector Backbone:pMpGWB102; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:23421791
Proper citation: RRID:Addgene_44592 Copy
Species: Mus musculus
Genetic Insert: CEBPalpha
Vector Backbone Description: Backbone Marker:Warren Pear lab; Backbone Size:6500; Vector Backbone:NGFR (Addgene plasmid 27489); Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23444353
Proper citation: RRID:Addgene_44627 Copy
Species: Homo sapiens
Genetic Insert: K8
Vector Backbone Description: Vector Backbone:pCIN4-Puro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Please note that Addgene's sequencing results identified two silent mutations in K8 when compared to GenBank accession NM_002273.3. The protein sequence is not affected by these differences.
Proper citation: RRID:Addgene_44626 Copy
Species: Synthetic
Genetic Insert: Citrine-NLS
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3374; Vector Backbone:pDONR207; Vector Types:; Bacterial Resistance:Gentamicin
Defining Citation: PMID:23421791
Proper citation: RRID:Addgene_44586 Copy
Species: Homo sapiens
Genetic Insert: K8
Vector Backbone Description: Vector Backbone:pECE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:2471065
Proper citation: RRID:Addgene_44621 Copy
Species: Homo sapiens
Genetic Insert: K18
Vector Backbone Description: Vector Backbone:pGEM-1; Vector Types:Cloning; Bacterial Resistance:Ampicillin
Defining Citation: PMID:2434380
Proper citation: RRID:Addgene_44623 Copy
Species: Homo sapiens
Genetic Insert: chimeric TRF
Vector Backbone Description: Vector Backbone:pBabe; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23389450
Comments: TRF2 construct in which the DNA-binding domain (aa444-500 when compared to NP_005643.1) was replaced with the TRF1 MYB domain (aa337-439 when compared to NP_059523.2).
According to the depositing laboratory, the deletion of A434 does not affect TRF2 function.
Proper citation: RRID:Addgene_44575 Copy
Species: Homo sapiens
Genetic Insert: chimeric TRF
Vector Backbone Description: Vector Backbone:pBabe; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23389450
Comments: TRF2 construct in which the Hinge domain (aa254-443 when compared to NP_005643.1) was replaced with Hinge domain of TRF1 (aa265-376 when compared to NP_059523.2)
Proper citation: RRID:Addgene_44574 Copy
Species: Homo sapiens
Genetic Insert: chimeric TRF
Vector Backbone Description: Vector Backbone:pBabe; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23389450
Comments: TRF chimera with iDDR region of TRF2 (aa 407-431 compared to GenBank accession number NP_005643.1 - PKGKWNSSNGVEEKETWVEEDELFQ) replacing aa 346-364 of TRF1 (NP_059523.2).
Proper citation: RRID:Addgene_44576 Copy
Species: Homo sapiens
Genetic Insert: KRAS 3'UTR
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4281; Vector Backbone:pGL4.75; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: A substitute for pGL3-KRAS LCS6m in Chin et al., 2008.
Proper citation: RRID:Addgene_44571 Copy
Species: Mus musculus
Genetic Insert: endoB
Vector Backbone Description: Vector Backbone:pUC9; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:2416755
Comments: Original mouse EndoB (k18) cDNA
Proper citation: RRID:Addgene_44602 Copy
Vector Backbone Description: Backbone Size:4954; Vector Backbone:pVV16, pSE100, pMC1m; Vector Types:Bacterial Expression, E.coli-mycobacteria shuttle vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23315736
Proper citation: RRID:Addgene_44562 Copy
Vector Backbone Description: Vector Backbone:pVV16, pSE100, pMC1m; Vector Types:Bacterial Expression, E.coli-mycobacteria shuttle vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23315736
Proper citation: RRID:Addgene_44561 Copy
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