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Genetic Insert: Ptac-lacO
Vector Backbone Description: Backbone Size:3434; Vector Backbone:pKD13; Vector Types:; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23064344
Comments: Note that though there are some discrepancies between the Addgene quality control sequence and the depositor sequence, they should not affect the function of the plasmid.
Proper citation: RRID:Addgene_40180 Copy
Species: Synechococcus elongatus PCC 7942
Genetic Insert: PpsbAI::luxCDE
Vector Backbone Description: Vector Backbone:pAM1607; Vector Types:Cyanobacteria cloning vector; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:17417005
Comments: NSII vector
Lux Genes: luxCDE
Directs luciferase substrate synthesis in PCC7942. Synechococcus luxAB reporter strains glow without decanal addition
PpsbAI fragment as SalI-PvuII fragment from pAM1335 inserted upstream of luxCDE in SalI-StuI of pAM1607 (NSII, Km, luxCDE).
Proper citation: RRID:Addgene_40244 Copy
Genetic Insert: Kana_I-SceI
Vector Backbone Description: Vector Backbone:pcDNA3; Vector Types:; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:16526409
Proper citation: RRID:Addgene_41017 Copy
Vector Backbone Description: Backbone Size:4290; Vector Backbone:pTKIP; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:20047970
Proper citation: RRID:Addgene_41064 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: phiC31 attB
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3805; Vector Backbone:pCR2.1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:10801973
Proper citation: RRID:Addgene_18937 Copy
Genetic Insert: N terminal E tag with RBS
Vector Backbone Description: Backbone Size:2646; Vector Backbone:pMLL-KA; Vector Types:Synthetic Biology, 2ab assembly vector; Bacterial Resistance:Ampicillin and Kanamycin
Comments: Note that this plasmid needs to be grown in both Kanamycin and Ampicillin
Proper citation: RRID:Addgene_26009 Copy
Genetic Insert: N terminal 6X His tag with RBS
Vector Backbone Description: Backbone Size:2646; Vector Backbone:pMLL-KA; Vector Types:Synthetic Biology, 2ab assembly vector; Bacterial Resistance:Ampicillin and Kanamycin
Comments: Note that this plasmid needs to be grown in both Kanamycin and Ampicillin
Proper citation: RRID:Addgene_26007 Copy
Genetic Insert: N terminal T7 tag with RBS
Vector Backbone Description: Backbone Size:2646; Vector Backbone:pMLL-KA; Vector Types:Synthetic Biology, 2ab assembly vector; Bacterial Resistance:Ampicillin and Kanamycin
Comments: Note that this plasmid needs to be grown in both Kanamycin and Ampicillin
Proper citation: RRID:Addgene_26016 Copy
Genetic Insert: N terminal S tag with RBS
Vector Backbone Description: Backbone Size:2646; Vector Backbone:pMLL-KA; Vector Types:Synthetic Biology, 2ab assembly vector; Bacterial Resistance:Ampicillin and Kanamycin
Comments: Note that this plasmid needs to be grown in both Kanamycin and Ampicillin
Proper citation: RRID:Addgene_26014 Copy
Genetic Insert: N terminal myc tag with RBS
Vector Backbone Description: Backbone Size:2646; Vector Backbone:pMLL-KA; Vector Types:Synthetic Biology, 2ab assembly vector; Bacterial Resistance:Ampicillin and Kanamycin
Comments: Note that this plasmid needs to be grown in both Kanamycin and Ampicillin
Proper citation: RRID:Addgene_26013 Copy
Species: S. mediterranea (planarian)
Genetic Insert: pJC55.1-spp-16
Vector Backbone Description: Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:20967238
Comments: dsRNA can be generated by in vitro transcription with T7 RNA polymerase and single stranded probes can be generated with SP6 or T3 RNA polymerases.
The insert has minor sequence discrepancies with NCBI; these do not affect plasmid function.
Proper citation: RRID:Addgene_26490 Copy
Species: S. mediterranea (planarian)
Genetic Insert: 1020HH-1
Vector Backbone Description: Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:RNAi; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:20967238
Proper citation: RRID:Addgene_26521 Copy
Genetic Insert: spp-14
Vector Backbone Description: Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:RNAi; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:20967238
Proper citation: RRID:Addgene_26520 Copy
Species: S. mediterranea (planarian)
Genetic Insert: eye53-1
Vector Backbone Description: Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:RNAi; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:20967238
Comments: Addgene's quality control sequence has minor discrepancies with the provided depositor's sequence; these do not affect plasmid function.
Proper citation: RRID:Addgene_26533 Copy
Species: S. mediterranea (planarian)
Genetic Insert: npp-12
Vector Backbone Description: Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:20967238
Comments: dsRNA can be generated by in vitro transcription with T7 RNA polymerase and single stranded probes can be generated with SP6 or T3 RNA polymerases.
Proper citation: RRID:Addgene_26499 Copy
Species: S. mediterranea (planarian)
Genetic Insert: spp-18
Vector Backbone Description: Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:20967238
Comments: dsRNA can be generated by in vitro transcription with T7 RNA polymerase and single stranded probes can be generated with SP6 or T3 RNA polymerases.
Proper citation: RRID:Addgene_26496 Copy
Species: S. mediterranea (planarian)
Genetic Insert: grh-1
Vector Backbone Description: Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:20967238
Comments: dsRNA can be generated by in vitro transcription with T7 RNA polymerase and single stranded probes can be generated with SP6 or T3 RNA polymerases.
Proper citation: RRID:Addgene_26495 Copy
Species: S. mediterranea (planarian)
Genetic Insert: spp-13
Vector Backbone Description: Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:20967238
Comments: dsRNA can be generated by in vitro transcription with T7 RNA polymerase and single stranded probes can be generated with SP6 or T3 RNA polymerases.
Proper citation: RRID:Addgene_26498 Copy
Species: S. mediterranea (planarian)
Genetic Insert: pJC154.1-spp-19
Vector Backbone Description: Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:20967238
Comments: dsRNA can be generated by in vitro transcription with T7 RNA polymerase and single stranded probes can be generated with SP6 or T3 RNA polymerases.
The insert has minor sequence discrepancies with NCBI; these do not affect plasmid function.
Proper citation: RRID:Addgene_26494 Copy
Species: S. mediterranea (planarian)
Genetic Insert: spp-10
Vector Backbone Description: Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:RNAi; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:20967238
Proper citation: RRID:Addgene_26504 Copy
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