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Genetic Insert: CCAT-[MTS from S. cerevisiae ATP synthase subunit alpha (YBL099W)]-AATG
Vector Backbone Description: Vector Backbone:pUPD2; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29132331
Proper citation: RRID:Addgene_101696 Copy
Species: Other
Genetic Insert: intB13 integrase casette
Vector Backbone Description: Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30853419
Proper citation: RRID:Addgene_120230 Copy
Species: Synthetic
Genetic Insert: SpCas9-NG
Vector Backbone Description: Vector Backbone:pdCas9-bacteria; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_120228 Copy
Species: Synthetic
Genetic Insert: SpCas9-VQR
Vector Backbone Description: Vector Backbone:pdCas9-bacteria; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Comments: The TetR protein coding sequence contains an F178S difference compared to the reference sequence that does not impact the function of the plasmid.
Proper citation: RRID:Addgene_120224 Copy
Species: Synthetic
Genetic Insert: SpCas9-NG
Vector Backbone Description: Vector Backbone:pdCas9-bacteria; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_120225 Copy
Species: Escherichia coli
Genetic Insert: CRISPR spacers targeting IS1, IS5, IS3
Vector Backbone Description: Backbone Size:9326; Vector Backbone:pCRISPathBrick; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31008359
Comments: Please visit https://www.biorxiv.org/content/early/2018/09/27/428029 for BioRxiv preprint.
Proper citation: RRID:Addgene_120425 Copy
Species: Escherichia coli
Genetic Insert: CRISPR spacers targeting IS1, IS5, IS3, IS150
Vector Backbone Description: Backbone Size:9326; Vector Backbone:pCRISPathBrick; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31008359
Comments: Please visit https://www.biorxiv.org/content/early/2018/09/27/428029 for BioRxiv preprint.
Proper citation: RRID:Addgene_120424 Copy
Species: Synthetic
Genetic Insert: red fluorescent protein (mCherry), codon-optimized for C. difficile
Vector Backbone Description: Backbone Size:6400; Vector Backbone:pRFP185; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25527559
Proper citation: RRID:Addgene_120812 Copy
Species: Synthetic
Genetic Insert: red fluorescent protein (mCherry), codon-optimized for C. difficile, multiple-cloning site for protein fusion
Vector Backbone Description: Backbone Size:6400; Vector Backbone:pRFP185; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25527559
Proper citation: RRID:Addgene_120813 Copy
Species: Synthetic
Genetic Insert: cyan fluorescent protein, codon-optimized for low GC organisms
Vector Backbone Description: Backbone Size:6400; Vector Backbone:pRFP185; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24727226
Proper citation: RRID:Addgene_120814 Copy
Genetic Insert: Pecf15_up436 (-60 to +20)
Vector Backbone Description: Vector Backbone:ColE2 plasmid; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30575748
Proper citation: RRID:Addgene_120893 Copy
Species: Synthetic
Genetic Insert: Noncoding sequences for CRISPR-Cas12g1
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:4327; Vector Backbone:pACYC184; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30523077
Comments: For more information, please visit us at https://arbor.bio/. For commercial use or questions, please contact us at [email protected].
Proper citation: RRID:Addgene_120880 Copy
Species: Synthetic
Genetic Insert: RFP
Vector Backbone Description: Backbone Size:2168; Vector Backbone:pBb; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30870592
Comments: Cloning method was In-Fusion. Please note that a 1bp insertion following the CAT promoter was identified by Addgene's NGS result. The depositor noted that this discrepancy does NOT affect plasmid function.
Proper citation: RRID:Addgene_126110 Copy
Species: Mus musculus
Genetic Insert: Zif268 in pB1H1
Vector Backbone Description: Backbone Size:3527; Vector Backbone:pB1H1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:16041365
Proper citation: RRID:Addgene_12612 Copy
Species: Synthetic
Genetic Insert: RFP
Vector Backbone Description: Backbone Size:2168; Vector Backbone:pBb; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30870592
Comments: Cloning method was In-Fusion.
Proper citation: RRID:Addgene_126108 Copy
Species: Synthetic
Genetic Insert: RFP
Vector Backbone Description: Backbone Size:2168; Vector Backbone:pBb; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30870592
Comments: Cloning method was In-Fusion. Please note that a 1bp insertion following the CAT promoter was identified by Addgene's NGS result. The depositor noted that this discrepancy does NOT affect plasmid function.
Proper citation: RRID:Addgene_126109 Copy
Species: Synthetic
Genetic Insert: RFP
Vector Backbone Description: Backbone Size:2181; Vector Backbone:pBb; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30870592
Comments: Cloning method was In-Fusion.
Proper citation: RRID:Addgene_126171 Copy
Species: Synthetic
Genetic Insert: RFP
Vector Backbone Description: Backbone Size:2181; Vector Backbone:pBb; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30870592
Comments: Cloning method was In-Fusion.
Proper citation: RRID:Addgene_126168 Copy
Species: Synthetic
Genetic Insert: RFP
Vector Backbone Description: Backbone Size:2181; Vector Backbone:pBb; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30870592
Comments: Cloning method was In-Fusion.
Proper citation: RRID:Addgene_126169 Copy
Species: Synthetic
Genetic Insert: RFP
Vector Backbone Description: Backbone Size:2181; Vector Backbone:pBb; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30870592
Comments: Cloning method was In-Fusion.
Proper citation: RRID:Addgene_126154 Copy
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