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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pBS ZfHoxb1a Resource Report Resource Website 1+ mentions |
RRID:Addgene_27519 | Hoxb1a | Danio rerio | Ampicillin | Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript II; Vector Types:Riboprobe synthesis; Bacterial Resistance:Ampicillin | 2026-08-15 01:13:04 | 1 | |||
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pBS ZfHoxa5a Resource Report Resource Website |
RRID:Addgene_27517 | Hoxa5a | Danio rerio | Ampicillin | Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript II; Vector Types:Riboprobe synthesis; Bacterial Resistance:Ampicillin | 2026-08-15 01:13:04 | 0 | |||
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pBS ZfHoxa1a Resource Report Resource Website |
RRID:Addgene_27513 | Hoxa1a | Danio rerio | Ampicillin | Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript; Vector Types:Riboprobe production; Bacterial Resistance:Ampicillin | 2026-08-15 01:13:04 | 0 | |||
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pBS ZfHoxa2b Resource Report Resource Website |
RRID:Addgene_27514 | Hoxa2b | Danio rerio | Ampicillin | Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript SK-; Vector Types:Riboprobe synthesis; Bacterial Resistance:Ampicillin | 2026-08-15 01:13:04 | 0 | |||
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pBS ZfHoxb9a Resource Report Resource Website |
RRID:Addgene_27529 | Hoxb9a | Danio rerio | Ampicillin | PMID:9425136 | Backbone Size:3000; Vector Backbone:pBluescript; Vector Types:Cloning vector; Bacterial Resistance:Ampicillin | 2026-08-15 01:13:03 | 0 | ||
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pBS ZfHoxb6b Resource Report Resource Website |
RRID:Addgene_27526 | Hoxb6b | Danio rerio | Ampicillin | Backbone Size:3000; Vector Backbone:pBluescript; Vector Types:Cloning vector; Bacterial Resistance:Ampicillin | 2026-08-15 01:13:04 | 0 | |||
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pBS ZfHoxb6a Resource Report Resource Website |
RRID:Addgene_27525 | Hoxb6a | Danio rerio | Ampicillin | Backbone Size:3000; Vector Backbone:pBluescript; Vector Types:Cloning vector; Bacterial Resistance:Ampicillin | 2026-08-15 01:13:04 | 0 | |||
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pBS ZfHoxb1b Resource Report Resource Website |
RRID:Addgene_27520 | Hoxb1b | Danio rerio | Ampicillin | Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript II; Vector Types:Riboprobe synthesis; Bacterial Resistance:Ampicillin | 2026-08-15 01:13:04 | 0 | |||
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CACNA1D (2of2) or cav1.3b_R (OZ548) Resource Report Resource Website |
RRID:Addgene_28073 | Zinc finger array targeting CACNA1D (2of2) or cav1.3b | Danio rerio | Kanamycin | PMID:18657511 | This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene CACNA1D (2of2) or cav1.3b (see below). Please note that this plasmid does NOT contain the CACNA1D (2of2) or cav1.3b sequence. Users must order the complementary plasmid CACNA1D (2of2) or cav1.3b_L (OZ547) [Addgene plasmid 28072] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-GCGGCCGAA-3' in a bacterial two hybrid assay, and resulted in 5.30 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934 | Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin | 2026-08-15 01:13:09 | 0 | |
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histamine receptor H3 (hrh3)_L (OZ549) Resource Report Resource Website |
RRID:Addgene_28074 | Zinc finger array targeting histamine receptor H3 (hrh3) | Danio rerio | Kanamycin | PMID:18657511 | This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene histamine receptor H3 (hrh3) (see below). Please note that this plasmid does NOT contain the histamine receptor H3 (hrh3) sequence. Users must order the complementary plasmid histamine receptor H3 (hrh3)_R (OZ550) [Addgene plasmid 28075] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-TCCGTGGCG-3' in a bacterial two hybrid assay, and resulted in 5.61 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934 | Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin | 2026-08-15 01:13:10 | 0 | |
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Swachmann-Bodian-Diamond Syndrome Gene, sbds_R (OZ546) Resource Report Resource Website |
RRID:Addgene_28071 | Zinc finger array targeting Swachmann-Bodian-Diamond Syndrome Gene, sbds | Danio rerio | Kanamycin | PMID:18657511 | This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene Swachmann-Bodian-Diamond Syndrome Gene, sbds (see below). Please note that this plasmid does NOT contain the sbds sequence. Users must order the complementary plasmid Swachmann-Bodian-Diamond Syndrome Gene, sbds_L (OZ545) [Addgene plasmid 28070] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-GATGAAGAA-3' in a bacterial two hybrid assay, and resulted in 6.00 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934 | Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin | 2026-08-15 01:13:10 | 0 | |
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CACNA1D (2of2) or cav1.3b_L (OZ547) Resource Report Resource Website |
RRID:Addgene_28072 | Zinc finger array targeting CACNA1D (2of2) or cav1.3b | Danio rerio | Kanamycin | PMID:18657511 | This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene CACNA1D (2of2) or cav1.3b (see below). Please note that this plasmid does NOT contain the CACNA1D (2of2) or cav1.3b sequence. Users must order the complementary plasmid CACNA1D (2of2) or cav1.3b_R (OZ548) [Addgene plasmid 28073] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-GACGGAGCT-3' in a bacterial two hybrid assay, and resulted in 6.02 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934 | Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin | 2026-08-15 01:13:10 | 0 | |
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Swachmann-Bodian-Diamond Syndrome Gene, sbds_L (OZ545) Resource Report Resource Website |
RRID:Addgene_28070 | Zinc finger array targeting Swachmann-Bodian-Diamond Syndrome Gene, sbds | Danio rerio | Kanamycin | PMID:18657511 | This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene Swachmann-Bodian-Diamond Syndrome Gene, sbds (see below). Please note that this plasmid does NOT contain the sbds sequence. Users must order the complementary plasmid Swachmann-Bodian-Diamond Syndrome Gene, sbds_R (OZ546) [Addgene plasmid 28071] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-GACGGCAAC-3' in a bacterial two hybrid assay, and resulted in 6.55 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934 | Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin | 2026-08-15 01:13:09 | 0 | |
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NFATc1_L (OZ543) Resource Report Resource Website |
RRID:Addgene_28068 | Zinc finger array targeting NFATc1 | Danio rerio | Kanamycin | PMID:18657511 | This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene NFATc1(see below). Please note that this plasmid does NOT contain the NFATc1 sequence. Users must order the complementary plasmid NFATc1_R (OZ544) [Addgene plasmid 28069] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-GAGGCGAGG-3' in a bacterial two hybrid assay, and resulted in 8.93 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934 | Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin | 2026-08-15 01:13:10 | 0 | |
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Encephalopsin (Opn3)_R (OZ542) Resource Report Resource Website |
RRID:Addgene_28067 | Zinc finger array targeting Encephalopsin (Opn3) | Danio rerio | Kanamycin | PMID:18657511 | This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene Encephalopsin (Opn3)(see below). Please note that this plasmid does NOT contain the Encephalopsin (Opn3) sequence. Users must order the complementary plasmid Encephalopsin (Opn3)_L (OZ541) [Addgene plasmid 28066] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-GTGGCAGAA-3' in a bacterial two hybrid assay, and resulted in 3.30 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934 | Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin | 2026-08-15 01:13:09 | 0 | |
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vipr1_L (OZ559) Resource Report Resource Website |
RRID:Addgene_28084 | Zinc finger array targeting vipr1 | Danio rerio | Kanamycin | PMID:18657511 | This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene vipr1 (see below). Please note that this plasmid does NOT contain the vipr1 sequence. Users must order the complementary plasmid vipr_R (OZ560) [Addgene plasmid 28085] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-GCTGATGGC-3' in a bacterial two hybrid assay, and resulted in 7.61 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934 | Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin | 2026-08-15 01:13:10 | 0 | |
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vipr1_R (OZ560) Resource Report Resource Website |
RRID:Addgene_28085 | Zinc finger array targeting vipr1 | Danio rerio | Kanamycin | PMID:18657511 | This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene vipr1 (see below). Please note that this plasmid does NOT contain the vipr1 sequence. Users must order the complementary plasmid vipr_L (OZ559) [Addgene plasmid 28084] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-GGAGAAGTA-3' in a bacterial two hybrid assay, and resulted in 7.29 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934 | Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin | 2026-08-15 01:13:09 | 0 | |
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Atg7_L (OZ557) Resource Report Resource Website |
RRID:Addgene_28082 | Zinc finger array targeting Atg7 | Danio rerio | Kanamycin | PMID:18657511 | This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene Atg7 (see below). Please note that this plasmid does NOT contain the Atg7 sequence. Users must order the complementary plasmid Atg7_R (OZ558) [Addgene plasmid 28083] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-GCAGAAGTA-3' in a bacterial two hybrid assay, and resulted in 8.32 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934 | Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin | 2026-08-15 01:13:09 | 0 | |
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cdkn1a(p21)_R (OZ556) Resource Report Resource Website |
RRID:Addgene_28081 | Zinc finger array targeting cdkn1a(p21) | Danio rerio | Kanamycin | PMID:18657511 | This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene cdkn1a(p21) (see below). Please note that this plasmid does NOT contain the cdkn1a(p21) sequence. Users must order the complementary plasmid cdkn1a(p21)_L (OZ555) [Addgene plasmid 28080] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-TGGGCCGAC-3' in a bacterial two hybrid assay, and resulted in 8.48 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934 | Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin | 2026-08-15 01:13:10 | 0 | |
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GUSB_L (OZ551) Resource Report Resource Website |
RRID:Addgene_28076 | Zinc finger array targeting GUSB | Danio rerio | Kanamycin | PMID:18657511 | This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene GUSB (see below). Please note that this plasmid does NOT contain the GUSB sequence. Users must order the complementary plasmid GUSB_R (OZ552) [Addgene plasmid 28077] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-GCAGATGAC-3' in a bacterial two hybrid assay, and resulted in 8.72 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934 | Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin | 2026-08-15 01:13:09 | 0 |
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