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Species: Caenorhabditis elegans
Vector Backbone Description: Backbone Size:0; Vector Backbone:pBS; Vector Types:Bacterial Expression, Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20729841
Proper citation: RRID:Addgene_26386 Copy
Species: Caenorhabditis elegans
Vector Backbone Description: Backbone Size:0; Vector Backbone:pBS; Vector Types:Bacterial Expression, Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20729841
Proper citation: RRID:Addgene_26388 Copy
Species: Caenorhabditis elegans
Vector Backbone Description: Backbone Size:0; Vector Backbone:pBS; Vector Types:Bacterial Expression, Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20729841
Comments: PLEASE NOTE: this plasmid is approximately 1.6kb shorter than expected in the region between the T7 promoter and pBR322 origin region based on the author sequence. Both promoters are present in their entirety based on the Addgene Quality Control sequencing. According to the depositing laboratory the plasmid behaves as expected and gives the expected pattern of fluorescence and Neomycin resistant phenotype.
There are a few other mismatches with author sequence but they also do not affect function. For questions regarding this plasmid please contact the depositing scientist Denis Dupuy at [email protected]
AgeI is NOT a unique restriction site. A second site is present at around nucleotide 7210.
Proper citation: RRID:Addgene_26393 Copy
Species: Caenorhabditis elegans
Genetic Insert: cel-pre-let-7_LPB
Vector Backbone Description: Backbone Size:7556; Vector Backbone:MXW-PGK-let7-IRES-GFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20808284
Comments: BamHI and BstBI are endogenous sites that flank the hairpin region of wt cel-let-7.
Proper citation: RRID:Addgene_26548 Copy
Species: Caenorhabditis elegans
Genetic Insert: cel-pre-let-7_LPA
Vector Backbone Description: Backbone Size:7556; Vector Backbone:MXW-PGK-let7-IRES-GFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20808284
Comments: BamHI and BstBI are endogenous sites that flank the hairpin region of wt cel-let-7.
Proper citation: RRID:Addgene_26547 Copy
Species: Caenorhabditis elegans
Genetic Insert: cel-pre-let-7_LPC
Vector Backbone Description: Backbone Size:7556; Vector Backbone:MXW-PGK-let7-IRES-GFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20808284
Comments: BamHI and BstBI are endogenous sites that flank the hairpin region of wt cel-let-7.
Proper citation: RRID:Addgene_26549 Copy
Species: Caenorhabditis elegans
Genetic Insert: lin-41_LCS-2G
Vector Backbone Description: Backbone Marker:Chang-Zheng Chen Lab; Backbone Size:4075; Vector Backbone:phRL-TKlkF; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20808284
Proper citation: RRID:Addgene_26544 Copy
Species: Caenorhabditis elegans
Genetic Insert: lin-41_LCS-2U
Vector Backbone Description: Backbone Marker:Chang-Zheng Chen Lab; Backbone Size:4075; Vector Backbone:phRL-TKlkF; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20808284
Proper citation: RRID:Addgene_26543 Copy
Species: Caenorhabditis elegans
Genetic Insert: cel-let-7 (~250nt flanking each side)
Vector Backbone Description: Backbone Marker:Chang-Zheng Chen Lab; Backbone Size:7060; Vector Backbone:MXW-PGK-IRES-GFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20808284
Proper citation: RRID:Addgene_26545 Copy
Species: Caenorhabditis elegans
Genetic Insert: lin-41_LCS-wt
Vector Backbone Description: Backbone Marker:Chang-Zheng Chen Lab; Backbone Size:4075; Vector Backbone:phRL-TKlkF; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20808284
Proper citation: RRID:Addgene_26542 Copy
Species: Caenorhabditis elegans
Genetic Insert: cel-pre-let-7_LPD
Vector Backbone Description: Backbone Size:7556; Vector Backbone:MXW-PGK-let7-IRES-GFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20808284
Comments: BamHI and BstBI are endogenous sites that flank the hairpin region of wt cel-let-7.
Proper citation: RRID:Addgene_26551 Copy
Species: Caenorhabditis elegans
Genetic Insert: lin-41_LCS-sm
Vector Backbone Description: Backbone Marker:Chang-Zheng Chen Lab; Backbone Size:4075; Vector Backbone:phRL-TKlkF; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20808284
Comments: Negative control for luciferase assays (to measure seed-dependent regulation). Renilla luciferase.
Proper citation: RRID:Addgene_26487 Copy
Species: Caenorhabditis elegans
Genetic Insert: pre-let-7_PS hairpin
Vector Backbone Description: Backbone Size:6946; Vector Backbone:MDH1-PGK-GFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20808284
Proper citation: RRID:Addgene_26537 Copy
Species: Caenorhabditis elegans
Genetic Insert: lin-41 3' UTR
Vector Backbone Description: Backbone Size:2742; Vector Backbone:pDPlk; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20808284
Proper citation: RRID:Addgene_26565 Copy
Species: Caenorhabditis elegans
Genetic Insert: cel-pre-let-7_LPD
Vector Backbone Description: Backbone Marker:Ambion; Backbone Size:2700; Vector Backbone:pDP19; Vector Types:transcription vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20808284
Comments: This construct does not produce perfect pre-let-7. There are leader and tail sequences from the backbone MCS. Used for in vitro translation.
Proper citation: RRID:Addgene_26562 Copy
Species: Caenorhabditis elegans
Genetic Insert: cel-pre-let-7_LPC
Vector Backbone Description: Backbone Marker:Ambion; Backbone Size:2700; Vector Backbone:pDP19; Vector Types:transcription vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20808284
Comments: This construct does not produce perfect pre-let-7. There are leader and tail sequences from the backbone MCS. Used for in vitro translation.
Proper citation: RRID:Addgene_26561 Copy
Species: Caenorhabditis elegans
Genetic Insert: lin-41 3' UTR
Vector Backbone Description: Backbone Size:2742; Vector Backbone:pDPlk; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20808284
Proper citation: RRID:Addgene_26564 Copy
Species: Caenorhabditis elegans
Genetic Insert: lsm-1
Vector Backbone Description: Backbone Size:0; Vector Backbone:unknown; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18692039
Proper citation: RRID:Addgene_26939 Copy
Species: Caenorhabditis elegans
Genetic Insert: cgh-1
Vector Backbone Description: Backbone Size:0; Vector Backbone:unknown; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18692039
Proper citation: RRID:Addgene_26940 Copy
Species: Caenorhabditis elegans
Genetic Insert: unc-119 rescuing fragment/pie-1promoter-gateway destination cassette B- pie-1 3’
Vector Backbone Description: Backbone Size:17037; Vector Backbone:pie-1; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18692039
Comments: Second Generation pie-1 vector for expressing ORFs in the germline
Proper citation: RRID:Addgene_26955 Copy
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