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Species: E.coli
Genetic Insert: E. coli lipoic acid ligase
Vector Backbone Description: Backbone Size:5190; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25313043
Proper citation: RRID:Addgene_61307 Copy
Genetic Insert: Ptac-dTomato
Vector Backbone Description: Vector Backbone:pAWP78; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25548049
Comments: dTomato reference:
Shaner, N. C., Campbell, R. E., Steinbach, P. A., Giepmans, B. N. G., Palmer, A. E., & Tsien, R. Y. (2004). Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nature Biotechnology, 22(12), 1567–1572. doi:10.1038/nbt1037
Proper citation: RRID:Addgene_61264 Copy
Vector Backbone Description: Backbone Size:4979; Vector Backbone:pCM66; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25548049
Comments: In order to use plasmid pAWP78, inserts should be added by Gibson Cloning. Primers to amplify the backbone have been annotated on the depositor's plasmid map. The sequences are (5' to 3'):
AP259_pAWP78_fwd1 - TTGTCGGGAAGATGCGTGAT
AP254_pAWP78_rev1 - CAGCTCACTCAAAGGCGGTA
Proper citation: RRID:Addgene_61263 Copy
Species: Synthetic
Genetic Insert: QF2w
Vector Backbone Description: Vector Backbone:pGAWB; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25581800
Comments: Please see this additional reference https://www.ncbi.nlm.nih.gov/pubmed/20434990 for more information on the Q-system.
Proper citation: RRID:Addgene_61313 Copy
Species: Homo sapiens
Genetic Insert: MBNL1
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_61277 Copy
Species: Homo sapiens
Genetic Insert: CUG-BP
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_61276 Copy
Species: Homo sapiens
Genetic Insert: NOVA1
Vector Backbone Description: Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_61275 Copy
Genetic Insert: AtPDLP1
Vector Backbone Description: Backbone Marker:VIB; Vector Backbone:pK7m34GW; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:25329881
Proper citation: RRID:Addgene_61207 Copy
Species: Danio rerio
Genetic Insert: Slit1b
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pBSII SK(+); Vector Types:cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14579375
Comments: antisense probe: cut with SmaI and use T7.
Proper citation: RRID:Addgene_61280 Copy
Vector Backbone Description: Backbone Size:4653; Vector Backbone:pBAD18; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25326321
Proper citation: RRID:Addgene_61284 Copy
Species: Mus musculus
Genetic Insert: IL-6 promoter
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pGL2 basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12626566
Proper citation: RRID:Addgene_61286 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3.1(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25019686
Comments: Please note that the mammalian selection marker in the vector backbone for this plasmid has not been verified by sequencing.
Proper citation: RRID:Addgene_61347 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3.1(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25019686
Comments: Please note that the mammalian selection marker in the vector backbone for this plasmid has not been verified by sequencing.
Proper citation: RRID:Addgene_61346 Copy
Species: Synthetic
Genetic Insert: S.pyogenes dCas9 with c-terminal HA tag
Vector Backbone Description: Backbone Marker:Invitrogen; Life Technologies; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25849900
Proper citation: RRID:Addgene_61355 Copy
Vector Backbone Description: Backbone Size:6911; Vector Backbone:pNeuroD-Ires-GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19737524
Proper citation: RRID:Addgene_61403 Copy
Species: Homo sapiens
Genetic Insert: KCC2
Vector Backbone Description: Backbone Size:6900; Vector Backbone:pCITF; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19376067
Proper citation: RRID:Addgene_61404 Copy
Species: Homo sapiens
Genetic Insert: OTUD1
Vector Backbone Description: Backbone Marker:OPPF-UK; Backbone Size:6299; Vector Backbone:pOPINK; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23827681
Proper citation: RRID:Addgene_61405 Copy
Species: Homo sapiens
Genetic Insert: OTUD1
Vector Backbone Description: Backbone Marker:OPPF-UK; Backbone Size:6299; Vector Backbone:pOPINK; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23827681
Comments: Hospenthal MK, Mevissen TET, Komander D. Nature Protoc. 2015 Jan 29;10:349-361. doi:10.1038/nprot.2015.018
Proper citation: RRID:Addgene_61406 Copy
Species: Homo sapiens
Genetic Insert: S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300)
Vector Backbone Description: Backbone Marker:Invitrogen; Life Technologies; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25849900
Proper citation: RRID:Addgene_61362 Copy
Species: Synthetic
Genetic Insert: dCAS9(D10A, N863A)-VP64_2A_Blast
Vector Backbone Description: Vector Backbone:plenti; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25494202
Comments: IMPORTANT NOTE: If you intend to order the SAM library, this plasmid will come included with that order. Please do NOT order this plasmid if you are already planning to order the SAM library.
For additional information, protocols and an activator sgRNA design tool, visit our website:
http://sam.genome-engineering.org/
Proper citation: RRID:Addgene_61425 Copy
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