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Species: Caenorhabditis elegans
Genetic Insert: Mex-5 (RNAi resistant)
Vector Backbone Description: Backbone Marker:Addgene plasmid 17247; Backbone Size:6351; Vector Backbone:pCG150; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21925318
Comments: Dendra::MEX-5 constructs were constructed as follows. A 4.4 kb mex-5 promoter fragment based on Tenlen et al. (2008) was cloned into pDONRP4P1R (Invitrogen). Dendra2/TEV/S-peptide (Gallo et al., 2010) was cloned into pDONR201. A MEX-5 genomic fragment from the start ATG through 648 bp of 3'UTR was cloned into pDONRP2RP3. Exon 2 of mex-5 was recoded to be RNAi-resistant (Gen-Script) so as to allow depletion of endogenous MEX-5/6 without depletion of the transgene. These constructs were assembled into pCG150 using three-way Gateway system (LR reaction) (Invitrogen) (Merritt et al., 2008). Mutations were made by recombinant PCR and all inserts were sequenced verified.
GFP::MEX-5 transgenes were constructed in the same way as Dendra::MEX-5 constructs except a pDONR201-GFP/TEV/FLAG entry clone was used and wild-type sequence of MEX-5 exon 2 was used.
Alternate plasmid name: mex-5 prom::GFP/TEV/FLAG::MEX-5::mex-5 3'UTR
Proper citation: RRID:Addgene_40194 Copy
Species: Caenorhabditis elegans
Genetic Insert: par-3C+S950A
Vector Backbone Description: Backbone Marker:GE; Backbone Size:4900; Vector Backbone:pGEX-6p-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21983565
Comments: Alternate plasmid name: pGEX6p1-par-3C+S950A
Proper citation: RRID:Addgene_40162 Copy
Species: Caenorhabditis elegans
Genetic Insert: par-1N:FLAG
Vector Backbone Description: Backbone Marker:GE; Backbone Size:4900; Vector Backbone:pGEX-6p-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21983565
Comments: Alternate plasmid name: pGEX6p1-par-1N:FLAG
Proper citation: RRID:Addgene_40157 Copy
Species: Caenorhabditis elegans
Genetic Insert: par-2 RNAi resistant, 7SE
Vector Backbone Description: Backbone Marker:GE; Backbone Size:4900; Vector Backbone:pGEX-6p-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21983565
Comments: Alternate plasmid name: pGEX6p1-par-2 7SE
7SE mutations are: S190E, S197E, S241E, S265E, S295E, S301E, and S335E.
Note: Mutations T6A and S8A were discovered during the Addgene quality control process and are present in this plasmid.
Proper citation: RRID:Addgene_40156 Copy
Species: Caenorhabditis elegans
Genetic Insert: par-2 RNAi resistant, C56S
Vector Backbone Description: Backbone Marker:GE; Backbone Size:4900; Vector Backbone:pGEX-6p-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21983565
Comments: Alternate plasmid name: pGEX6p1-par-2 C56S
Proper citation: RRID:Addgene_40155 Copy
Species: Caenorhabditis elegans
Genetic Insert: par-2 RNAi resistant, R183-5A
Vector Backbone Description: Backbone Marker:GE; Backbone Size:4900; Vector Backbone:pGEX-6p-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21983565
Comments: Alternate plasmid name: pGEX6p1-par-2 R183-5A
Proper citation: RRID:Addgene_40150 Copy
Species: Caenorhabditis elegans
Genetic Insert: par-2 RNAi resistant, wild-type
Vector Backbone Description: Backbone Marker:GE; Backbone Size:4900; Vector Backbone:pGEX-6p-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21983565
Comments: Alternate plasmid name: pGEX6p1-par-2 wild-type
Proper citation: RRID:Addgene_40149 Copy
Species: Caenorhabditis elegans
Genetic Insert: gfp::par-2 RNAi resistant R183-5A
Vector Backbone Description: Backbone Marker:GE; Backbone Size:4900; Vector Backbone:pGEX-6p-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21983565
Comments: Alternate plasmid name: pGEX6p1-gfp::par-2 R183-5A
Proper citation: RRID:Addgene_40148 Copy
Species: Caenorhabditis elegans
Genetic Insert: gfp::par-2 wild-type RNAi resistant
Vector Backbone Description: Backbone Marker:GE; Backbone Size:4900; Vector Backbone:pGEX-6p-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21983565
Comments: Alternate plasmid name: pGEX6p1-gfp::par-2 wild-type
Proper citation: RRID:Addgene_40147 Copy
Species: Caenorhabditis elegans
Genetic Insert: par-2 RNAi resistant (S241A)
Vector Backbone Description: Backbone Marker:Addgene plasmid 22720; Backbone Size:18033; Vector Backbone:pID3.01; Vector Types:Worm Expression, Gateway Cloning; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21983565
Comments: Alternate plasmid name: pID3.01-par-2RR S241A
Proper citation: RRID:Addgene_40144 Copy
Species: Caenorhabditis elegans
Genetic Insert: par-2 RNAi resistant (R183-5A + S241A)
Vector Backbone Description: Backbone Marker:Addgene plasmid 22720; Backbone Size:18033; Vector Backbone:pID3.01; Vector Types:Worm Expression, Gateway Cloning; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21983565
Comments: Alternate plasmid name: pID3.01-par-2RR R183-5A + S241A
Proper citation: RRID:Addgene_40142 Copy
Species: Caenorhabditis elegans
Genetic Insert: par-2 RNAi resistant (R163A)
Vector Backbone Description: Backbone Marker:Addgene plasmid 22720; Backbone Size:18033; Vector Backbone:pID3.01; Vector Types:Worm Expression, Gateway Cloning; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21983565
Comments: Alternate plasmid name: pID3.01-par-2RR R163A
Proper citation: RRID:Addgene_40140 Copy
Species: Caenorhabditis elegans
Genetic Insert: par-2 RNAi resistant (K162A)
Vector Backbone Description: Backbone Marker:Addgene plasmid 22720; Backbone Size:18033; Vector Backbone:pID3.01; Vector Types:Worm Expression, Gateway Cloning; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21983565
Comments: Alternate plasmid name: pID3.01-par-2RR K162A
Proper citation: RRID:Addgene_40139 Copy
Species: Caenorhabditis elegans
Genetic Insert: Mex-5 (S404A, S458A)
Vector Backbone Description: Backbone Marker:New England Biolabs (Modified); Backbone Size:6700; Vector Backbone:pMal c2X (GW); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21925318
Comments: Alternate plasmid name: pMAL c2X (GW) + MEX-5 (S404A, S458A)
Proper citation: RRID:Addgene_40089 Copy
Species: Caenorhabditis elegans
Genetic Insert: Mex-5 (ATG through 648 bp of 3'UTR) (RNAi resistant exon 2)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4773; Vector Backbone:pDONR P2RP3; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21925318
Comments: A MEX-5 genomic fragment from the start ATG through 648 bp of 3'UTR was cloned into pDONRP2RP3. Exon 2 of mex-5 was recoded to be RNAi-resistant (Gen-Script) so as to allow depletion of endogenous MEX-5/6 without depletion of the transgene.
Alternate plasmid name: pDONR P2RP3+MEX-5 genomic(RNAI res exon 2) + 3’UTR
Proper citation: RRID:Addgene_40122 Copy
Species: Caenorhabditis elegans
Genetic Insert: Mex-5 (S404A)
Vector Backbone Description: Backbone Marker:New England Biolabs (Modified); Backbone Size:6700; Vector Backbone:pMal c2X (GW); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21925318
Comments: Alternate plasmid name: pMAL c2X (GW) + MEX-5 (S404A)
Proper citation: RRID:Addgene_40088 Copy
Species: Caenorhabditis elegans
Genetic Insert: Green fluorescent protein
Vector Backbone Description: Backbone Marker:Epicentre; Backbone Size:2050; Vector Backbone:pMOD4; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19116030
Proper citation: RRID:Addgene_19181 Copy
Species: Caenorhabditis elegans
Genetic Insert: Prab-3::mCherry
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2432; Vector Backbone:pDESTR4-R3; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18953339
Comments: Coinjection marker. mCherry expression in the nervous system.
Proper citation: RRID:Addgene_19359 Copy
Species: Caenorhabditis elegans
Genetic Insert: pos-1 3'utr in pDONRP2RP3
Vector Backbone Description: Backbone Size:2641; Vector Backbone:pDONRP2RP3; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_19370 Copy
Species: Caenorhabditis elegans
Genetic Insert: spe-41 3'utr in pDONRP2RP3
Vector Backbone Description: Backbone Size:2641; Vector Backbone:pDONRP2RP3; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_19373 Copy
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