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Species: E. coli
Genetic Insert: pLlacO-1:hfq, pLtetO-1m9:sodB::gfp
Vector Backbone Description: Backbone Marker:Lim Lab (modified from Rolf Lutz and Hermann Bujard, 1997); Backbone Size:2000; Vector Backbone:ColE (from pZ system) + AmpR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21189298
Comments: Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
Proper citation: RRID:Addgene_30099 Copy
Species: E. coli
Genetic Insert: pLlacO-1:hfq, pLtetO-1:rpoS::gfp
Vector Backbone Description: Backbone Marker:Lim Lab (modified from Rolf Lutz and Hermann Bujard, 1997); Backbone Size:2000; Vector Backbone:ColE (from pZ system) + AmpR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21189298
Comments: Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
Proper citation: RRID:Addgene_30098 Copy
Species: E. coli
Genetic Insert: pLtetO-1:RhyB, pLlacO-1:hfq, pLtetO-1m9:sodB::gfp
Vector Backbone Description: Backbone Marker:Lim Lab (modified from Rolf Lutz and Hermann Bujard, 1997); Backbone Size:2000; Vector Backbone:ColE (from pZ system) + AmpR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21189298
Comments: Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
Proper citation: RRID:Addgene_30093 Copy
Species: E. coli
Genetic Insert: pLtetO-1:DsrA, pCon:T7(st3) (900bps), pLlacO-1:rpoS::gfp
Vector Backbone Description: Backbone Marker:Lim Lab (modified from Rolf Lutz and Hermann Bujard, 1997); Backbone Size:2000; Vector Backbone:ColE (from pZ system) + AmpR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21189298
Comments: Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
Proper citation: RRID:Addgene_30094 Copy
Species: E. coli
Genetic Insert: pLtetO-1:RhyB, pLlacO-1:hfq, pLtetO-1m9:sodB::gfp
Vector Backbone Description: Backbone Marker:Lim Lab (modified from Rolf Lutz and Hermann Bujard, 1997); Backbone Size:2000; Vector Backbone:ColE (from pZ system) + AmpR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21189298
Comments: Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
Proper citation: RRID:Addgene_30086 Copy
Species: E. coli
Genetic Insert: pLtetO-1:rpoS::mCherry, pLlacO-1:ompC::gfp
Vector Backbone Description: Backbone Marker:Lim Lab (modified from Rolf Lutz and Hermann Bujard, 1997); Backbone Size:2000; Vector Backbone:ColE (from pZ system) + AmpR; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21189298
Comments: Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
Proper citation: RRID:Addgene_30088 Copy
Species: E. coli
Genetic Insert: E. coli elongation factor Tu
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5300; Vector Backbone:pET24c(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:8765000
Proper citation: RRID:Addgene_31172 Copy
Species: E. coli
Genetic Insert: E. coli Initiation Factor 3
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pET21c; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12095986
Proper citation: RRID:Addgene_31340 Copy
Species: E. coli
Genetic Insert: zapB
Vector Backbone Description: Backbone Size:5352; Vector Backbone:pBAD33; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:18394147
Proper citation: RRID:Addgene_31398 Copy
Species: E. coli
Genetic Insert: AdEasier-1 bacterial cells
Vector Backbone Description: Backbone Size:0; Vector Backbone:n/a; Vector Types:; Bacterial Resistance:Ampicillin and Streptomycin
Defining Citation: PMID:9482916
Comments: AdEasier-1 cells are BJ5183 derivatives which already contain the AdEasy-1 plasmid. Please note that you cannot isolate AdEasy-1 from this strain. Please see Plasmid #16400 if you only want the plasmid. In order to generate recombinant vectors ready to transfer into 293 cells, one simply has to transform the AdEasier-1 cells with a shuttle vector (such as pAdTrack-CMV) containing the gene of interest, and then proceed as with the standard AdEasy system.
AdEasier is a trademark of the Johns Hopkins University.
Proper citation: RRID:Addgene_16399 Copy
Species: E. coli
Genetic Insert: BJ5183 bacterial cells
Vector Backbone Description: Backbone Size:0; Vector Backbone:n/a; Vector Types:; Bacterial Resistance:Streptomycin
Defining Citation: PMID:9482916
Comments: This is a bacterial strain - not a plasmid. Select with 30 ug/mL streptomycin.
Electrocompetent BJ5183 cells are used to carry out the recombination between the shuttle vector (pShuttle, pShuttle-CMV, pAdTrack, or pAdTrack-CMV) and the adenoviral vector (pAdEasy1 or pAdEasy2).
Proper citation: RRID:Addgene_16398 Copy
Species: E. coli
Genetic Insert: MlaFEDCB
Vector Backbone Description: Vector Backbone:pBAD-His; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33236984
Proper citation: RRID:Addgene_164596 Copy
Species: E. coli
Genetic Insert: MlaFEDCB
Vector Backbone Description: Vector Backbone:pBAD-His; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33236984
Proper citation: RRID:Addgene_164598 Copy
Species: E. coli
Genetic Insert: MlaFEDCB
Vector Backbone Description: Vector Backbone:pBAD-His; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33236984
Proper citation: RRID:Addgene_164600 Copy
Species: E. coli
Genetic Insert: MlaFEDCB
Vector Backbone Description: Vector Backbone:pBAD-His; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33236984
Proper citation: RRID:Addgene_164603 Copy
Species: E. coli
Genetic Insert: LacI
Vector Backbone Description: Vector Backbone:pAN1818; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33784029
Comments: Derived from the pAN1818 plasmid from the Chris Voigt lab.
Proper citation: RRID:Addgene_164830 Copy
Species: E. coli
Genetic Insert: LacI
Vector Backbone Description: Vector Backbone:pAN1818; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33784029
Proper citation: RRID:Addgene_164831 Copy
Species: E. coli
Genetic Insert: E. coli gRNAs
Vector Backbone Description: Vector Backbone:psgRNA (Addgene #114005); Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32352514
Proper citation: RRID:Addgene_131625 Copy
Species: E. coli
Genetic Insert: Can
Vector Backbone Description: Backbone Size:2970; Vector Backbone:pFA; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31406332
Proper citation: RRID:Addgene_133010 Copy
Species: E. coli
Genetic Insert: axe/txe toxin-antitoxin cassette
Vector Backbone Description: Backbone Marker:Oxford Genetics; Backbone Size:4700; Vector Backbone:pSF_UC_OXB20-daGFP; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30954530
Proper citation: RRID:Addgene_133306 Copy
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