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Vector Backbone Description: Vector Backbone:pJOE; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34175462
Comments: Please note that the P10S mutation in the BLMT family bleomycin binding protein does not affect overall plasmid function.
Proper citation: RRID:Addgene_169245 Copy
Species: Synthetic
Genetic Insert: 14His-SUMO-nsp12
Vector Backbone Description: Vector Backbone:pK27Sumo; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34198323
Proper citation: RRID:Addgene_169188 Copy
Species: SARS-CoV-2
Genetic Insert: 14His-SUMO-nsp7
Vector Backbone Description: Vector Backbone:pK27Sumo; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34198323
Proper citation: RRID:Addgene_169186 Copy
Species: Synthetic
Genetic Insert: 14His-SUMO-nsp10-GSGSGS-nsp16
Vector Backbone Description: Vector Backbone:pK27SUMO; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34198328
Proper citation: RRID:Addgene_169191 Copy
Species: Synthetic
Genetic Insert: 14His-SUMO-nsp3_E746-K1060 (pp1ab_E1564-K1878)
Vector Backbone Description: Vector Backbone:pK27SUMO; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34198325
Proper citation: RRID:Addgene_169193 Copy
Vector Backbone Description: Backbone Marker:ExpreS2ion Biotechnologies; Backbone Size:2864; Vector Backbone:pExpreS2-1; Vector Types:Insect Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35600892
Comments: This plasmid is designed for use with the ExpreS2 Platform from ExpreS2ion Biotechnologies. It is intended to be used with the ExpreS2ion cells and media which can be obtained directly from the ExpreS2ion website.
Proper citation: RRID:Addgene_175362 Copy
Species: Homo sapiens
Genetic Insert: SDCBP
Vector Backbone Description: Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Ligation-independent cloning 5' site GTACTTCCAATCCAT; 3' site TATCCACCTTTACT. Gileadi lab uses BL21(DE3)-R3-pRARE2 cells for expression.
Proper citation: RRID:Addgene_175483 Copy
Species: Homo sapiens
Genetic Insert: SDCBP
Vector Backbone Description: Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Ligation-independent cloning 5' site GTACTTCCAATCCAT; 3' site TATCCACCTTTACT. Gileadi lab uses BL21(DE3)-R3-pRARE2 cells for expression.
Proper citation: RRID:Addgene_175484 Copy
Species: Homo sapiens
Genetic Insert: SDCBP
Vector Backbone Description: Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Ligation-independent cloning 5' site GTACTTCCAATCCAT; 3' site TATCCACCTTTACT. Gileadi lab uses BL21(DE3)-R3-pRARE2 cells for expression.
Proper citation: RRID:Addgene_175480 Copy
Species: Homo sapiens
Genetic Insert: SDCBP
Vector Backbone Description: Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Ligation-independent cloning 5' site GTACTTCCAATCCAT; 3' site TATCCACCTTTACT. Gileadi lab uses BL21(DE3)-R3-pRARE2 cells for expression.
Proper citation: RRID:Addgene_175482 Copy
Species: Homo sapiens
Genetic Insert: NF2
Vector Backbone Description: Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Ligation-independent cloning 5' site GTACTTCCAATCCAT; 3' site TATCCACCTTTACT. Gileadi lab uses BL21(DE3)-R3-pRARE2 cells for expression.
Proper citation: RRID:Addgene_175472 Copy
Species: Homo sapiens
Genetic Insert: PRDX1
Vector Backbone Description: Vector Backbone:pNIC-Bio3; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Ligation-independent cloning 5' site GTACTTCCAATCCAT; 3' site TATCCACCTTTACT. Gileadi lab uses BL21(DE3)-R3-pRARE2-pBirA cells for expression.
Please note: Plasmid is missing the GSGGGGSGGGGS linker sequence compared to the depositor's sequence. The missing extra linker between the full length coding sequence and Avitag does not affect function of the proteins.
Proper citation: RRID:Addgene_175473 Copy
Species: Homo sapiens
Genetic Insert: RAB5A
Vector Backbone Description: Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Ligation-independent cloning 5' site GTACTTCCAATCCAT; 3' site TATCCACCTTTACT. Gileadi lab uses BL21(DE3)-R3-pRARE2 cells for expression.
Proper citation: RRID:Addgene_175475 Copy
Species: Homo sapiens
Genetic Insert: RAB5A
Vector Backbone Description: Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Ligation-independent cloning 5' site GTACTTCCAATCCAT; 3' site TATCCACCTTTACT. Gileadi lab uses BL21(DE3)-R3-pRARE2 cells for expression.
Proper citation: RRID:Addgene_175476 Copy
Species: Homo sapiens
Genetic Insert: MSN
Vector Backbone Description: Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37866628
Comments: Ligation-independent cloning 5' site GTACTTCCAATCCAT; 3' site TATCCACCTTTACT. Gileadi lab uses BL21(DE3)-R3-pRARE2 cells for expression.
Proper citation: RRID:Addgene_175470 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: eRF3
Vector Backbone Description: Backbone Marker:UC Berkeley MacroLab; Vector Backbone:1C; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34413231
Proper citation: RRID:Addgene_175418 Copy
Species: Homo sapiens
Genetic Insert: CD44
Vector Backbone Description: Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Ligation-independent cloning 5' site GTACTTCCAATCCAT; 3' site TATCCACCTTTACT. Gileadi lab uses BL21(DE3)-R3-pRARE2 cells for expression.
Proper citation: RRID:Addgene_175448 Copy
Species: Homo sapiens
Genetic Insert: CD44
Vector Backbone Description: Vector Backbone:pNIC-CTH0; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Ligation-independent cloning 5' site GTACTTCCAATCCAT; 3' site TATCCACCTTTACT. Gileadi lab uses BL21(DE3)-R3-pRARE2 cells for expression.
Proper citation: RRID:Addgene_175449 Copy
Vector Backbone Description: Backbone Marker:Genscript; Backbone Size:4361; Vector Backbone:pBR322; Vector Types:Mouse Targeting, Cloning vector, to be used to deliver transgene into mice with dual heterologous Bxb1 attP sites; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35361849
Comments: We recommend this low-copy plasmid for the generation of donor DNA when the inserted transgene size is >15kb. For smaller transgene insertions, we recommend the high-copy plasmid p5154 (Addgene 175390).
Proper citation: RRID:Addgene_175391 Copy
Species: Homo sapiens
Genetic Insert: EZR
Vector Backbone Description: Vector Backbone:pNIC28-Bsa4; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Ligation-independent cloning 5' site GTACTTCCAATCCAT; 3' site TATCCACCTTTACT. Gileadi lab uses BL21(DE3)-R3-pRARE2 cells for expression.
Proper citation: RRID:Addgene_175461 Copy
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