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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pEG608 Resource Report Resource Website |
RRID:Addgene_40194 | Mex-5 (RNAi resistant) | Caenorhabditis elegans | Ampicillin | PMID:21925318 | Dendra::MEX-5 constructs were constructed as follows. A 4.4 kb mex-5 promoter fragment based on Tenlen et al. (2008) was cloned into pDONRP4P1R (Invitrogen). Dendra2/TEV/S-peptide (Gallo et al., 2010) was cloned into pDONR201. A MEX-5 genomic fragment from the start ATG through 648 bp of 3'UTR was cloned into pDONRP2RP3. Exon 2 of mex-5 was recoded to be RNAi-resistant (Gen-Script) so as to allow depletion of endogenous MEX-5/6 without depletion of the transgene. These constructs were assembled into pCG150 using three-way Gateway system (LR reaction) (Invitrogen) (Merritt et al., 2008). Mutations were made by recombinant PCR and all inserts were sequenced verified. GFP::MEX-5 transgenes were constructed in the same way as Dendra::MEX-5 constructs except a pDONR201-GFP/TEV/FLAG entry clone was used and wild-type sequence of MEX-5 exon 2 was used. Alternate plasmid name: mex-5 prom::GFP/TEV/FLAG::MEX-5::mex-5 3'UTR | Backbone Marker:Addgene plasmid 17247; Backbone Size:6351; Vector Backbone:pCG150; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin | Exon 2 recoded to be RNAi-resistant | 2026-08-15 01:14:34 | 0 |
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pFM116 Resource Report Resource Website |
RRID:Addgene_40162 | par-3C+S950A | Caenorhabditis elegans | Ampicillin | PMID:21983565 | Alternate plasmid name: pGEX6p1-par-3C+S950A | Backbone Marker:GE; Backbone Size:4900; Vector Backbone:pGEX-6p-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | C terminal portion of protein (aa840-1268); S950A | 2026-08-15 01:14:34 | 0 |
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pFM111 Resource Report Resource Website |
RRID:Addgene_40157 | par-1N:FLAG | Caenorhabditis elegans | Ampicillin | PMID:21983565 | Alternate plasmid name: pGEX6p1-par-1N:FLAG | Backbone Marker:GE; Backbone Size:4900; Vector Backbone:pGEX-6p-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | Contains aa 1-964 | 2026-08-15 01:14:34 | 0 |
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pFM110 Resource Report Resource Website |
RRID:Addgene_40156 | par-2 RNAi resistant, 7SE | Caenorhabditis elegans | Ampicillin | PMID:21983565 | Alternate plasmid name: pGEX6p1-par-2 7SE 7SE mutations are: S190E, S197E, S241E, S265E, S295E, S301E, and S335E. Note: Mutations T6A and S8A were discovered during the Addgene quality control process and are present in this plasmid. | Backbone Marker:GE; Backbone Size:4900; Vector Backbone:pGEX-6p-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 7SE; base pairs 6-523 of par-2 were recoded to create the RNAi-resistant par-2 transgene. | 2026-08-15 01:14:34 | 0 |
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pFM109 Resource Report Resource Website |
RRID:Addgene_40155 | par-2 RNAi resistant, C56S | Caenorhabditis elegans | Ampicillin | PMID:21983565 | Alternate plasmid name: pGEX6p1-par-2 C56S | Backbone Marker:GE; Backbone Size:4900; Vector Backbone:pGEX-6p-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | C56S; base pairs 6-523 of par-2 were recoded to create the RNAi-resistant par-2 transgene. | 2026-08-15 01:14:34 | 0 |
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pFM104 Resource Report Resource Website |
RRID:Addgene_40150 | par-2 RNAi resistant, R183-5A | Caenorhabditis elegans | Ampicillin | PMID:21983565 | Alternate plasmid name: pGEX6p1-par-2 R183-5A | Backbone Marker:GE; Backbone Size:4900; Vector Backbone:pGEX-6p-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | R183-5A; base pairs 6-523 of par-2 were recoded to create the RNAi-resistant par-2 transgene. | 2026-08-15 01:14:34 | 0 |
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pFM103 Resource Report Resource Website |
RRID:Addgene_40149 | par-2 RNAi resistant, wild-type | Caenorhabditis elegans | Ampicillin | PMID:21983565 | Alternate plasmid name: pGEX6p1-par-2 wild-type | Backbone Marker:GE; Backbone Size:4900; Vector Backbone:pGEX-6p-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | Base pairs 6-523 of par-2 were recoded to create the RNAi-resistant par-2 transgene. | 2026-08-15 01:14:34 | 0 |
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pFM102 Resource Report Resource Website |
RRID:Addgene_40148 | gfp::par-2 RNAi resistant R183-5A | Caenorhabditis elegans | Ampicillin | PMID:21983565 | Alternate plasmid name: pGEX6p1-gfp::par-2 R183-5A | Backbone Marker:GE; Backbone Size:4900; Vector Backbone:pGEX-6p-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | R183-5A; base pairs 6-523 of par-2 were recoded to create the RNAi-resistant par-2 transgene. | 2026-08-15 01:14:34 | 0 |
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pFM101 Resource Report Resource Website |
RRID:Addgene_40147 | gfp::par-2 wild-type RNAi resistant | Caenorhabditis elegans | Ampicillin | PMID:21983565 | Alternate plasmid name: pGEX6p1-gfp::par-2 wild-type | Backbone Marker:GE; Backbone Size:4900; Vector Backbone:pGEX-6p-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | Base pairs 6-523 of par-2 were recoded to create the RNAi-resistant par-2 transgene. | 2026-08-15 01:14:34 | 0 |
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pFM041 Resource Report Resource Website |
RRID:Addgene_40144 | par-2 RNAi resistant (S241A) | Caenorhabditis elegans | Ampicillin | PMID:21983565 | Alternate plasmid name: pID3.01-par-2RR S241A | Backbone Marker:Addgene plasmid 22720; Backbone Size:18033; Vector Backbone:pID3.01; Vector Types:Worm Expression, Gateway Cloning; Bacterial Resistance:Ampicillin | S241A; base pairs 6-523 of par-2 were recoded to create the RNAi-resistant par-2 transgene. | 2026-08-15 01:14:34 | 0 |
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pFM039 Resource Report Resource Website |
RRID:Addgene_40142 | par-2 RNAi resistant (R183-5A + S241A) | Caenorhabditis elegans | Ampicillin | PMID:21983565 | Alternate plasmid name: pID3.01-par-2RR R183-5A + S241A | Backbone Marker:Addgene plasmid 22720; Backbone Size:18033; Vector Backbone:pID3.01; Vector Types:Worm Expression, Gateway Cloning; Bacterial Resistance:Ampicillin | R183-5A + S241A; base pairs 6-523 of par-2 were recoded to create the RNAi-resistant par-2 transgene. | 2026-08-15 01:14:33 | 0 |
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pFM037 Resource Report Resource Website |
RRID:Addgene_40140 | par-2 RNAi resistant (R163A) | Caenorhabditis elegans | Ampicillin | PMID:21983565 | Alternate plasmid name: pID3.01-par-2RR R163A | Backbone Marker:Addgene plasmid 22720; Backbone Size:18033; Vector Backbone:pID3.01; Vector Types:Worm Expression, Gateway Cloning; Bacterial Resistance:Ampicillin | R163A; base pairs 6-523 of par-2 were recoded to create the RNAi-resistant par-2 transgene. | 2026-08-15 01:14:33 | 0 |
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pFM036 Resource Report Resource Website |
RRID:Addgene_40139 | par-2 RNAi resistant (K162A) | Caenorhabditis elegans | Ampicillin | PMID:21983565 | Alternate plasmid name: pID3.01-par-2RR K162A | Backbone Marker:Addgene plasmid 22720; Backbone Size:18033; Vector Backbone:pID3.01; Vector Types:Worm Expression, Gateway Cloning; Bacterial Resistance:Ampicillin | K162A; base pairs 6-523 of par-2 were recoded to create the RNAi-resistant par-2 transgene. | 2026-08-15 01:14:33 | 0 |
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pEG495 Resource Report Resource Website |
RRID:Addgene_40089 | Mex-5 (S404A, S458A) | Caenorhabditis elegans | Ampicillin | PMID:21925318 | Alternate plasmid name: pMAL c2X (GW) + MEX-5 (S404A, S458A) | Backbone Marker:New England Biolabs (Modified); Backbone Size:6700; Vector Backbone:pMal c2X (GW); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | S404A, S458A | 2026-08-15 01:14:33 | 0 |
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pEG795 Resource Report Resource Website |
RRID:Addgene_40122 | Mex-5 (ATG through 648 bp of 3'UTR) (RNAi resistant exon 2) | Caenorhabditis elegans | Kanamycin | PMID:21925318 | A MEX-5 genomic fragment from the start ATG through 648 bp of 3'UTR was cloned into pDONRP2RP3. Exon 2 of mex-5 was recoded to be RNAi-resistant (Gen-Script) so as to allow depletion of endogenous MEX-5/6 without depletion of the transgene. Alternate plasmid name: pDONR P2RP3+MEX-5 genomic(RNAI res exon 2) + 3’UTR | Backbone Marker:Invitrogen; Backbone Size:4773; Vector Backbone:pDONR P2RP3; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin | Exon 2 recoded to be RNAi-resistant | 2026-08-15 01:14:33 | 0 |
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pEG459 Resource Report Resource Website |
RRID:Addgene_40088 | Mex-5 (S404A) | Caenorhabditis elegans | Ampicillin | PMID:21925318 | Alternate plasmid name: pMAL c2X (GW) + MEX-5 (S404A) | Backbone Marker:New England Biolabs (Modified); Backbone Size:6700; Vector Backbone:pMal c2X (GW); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | S404A | 2026-08-15 01:14:33 | 0 |
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pMOD4 GFP Resource Report Resource Website |
RRID:Addgene_19181 | Green fluorescent protein | Caenorhabditis elegans | Ampicillin | PMID:19116030 | Backbone Marker:Epicentre; Backbone Size:2050; Vector Backbone:pMOD4; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin | S65C, contains synthetic introns for C. elegans | 2026-08-15 01:11:42 | 0 | |
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pGH8 - pRAB-3::mCherry::unc-54utr Resource Report Resource Website 10+ mentions |
RRID:Addgene_19359 | Prab-3::mCherry | Caenorhabditis elegans | Ampicillin | PMID:18953339 | Coinjection marker. mCherry expression in the nervous system. | Backbone Marker:Invitrogen; Backbone Size:2432; Vector Backbone:pDESTR4-R3; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:11:44 | 43 | |
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pCM5.66 Resource Report Resource Website |
RRID:Addgene_19370 | pos-1 3'utr in pDONRP2RP3 | Caenorhabditis elegans | Kanamycin | PMID:18818082 | Backbone Size:2641; Vector Backbone:pDONRP2RP3; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:11:44 | 0 | ||
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pCM5.63 Resource Report Resource Website |
RRID:Addgene_19373 | spe-41 3'utr in pDONRP2RP3 | Caenorhabditis elegans | Kanamycin | PMID:18818082 | Backbone Size:2641; Vector Backbone:pDONRP2RP3; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:11:44 | 0 |
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