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  • Organism:danio rerio (facet)


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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
NRF2b (nfe212b)_L (OZ561)
 
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RRID:Addgene_28086 Zinc finger array targeting NRF2b (nfe212b) Danio rerio Kanamycin PMID:18657511 This plasmid encodes a zinc finger array targeting half of a target sequence in the zebrafish gene NRF2b (nfe212b) (see below). Please note that this plasmid does NOT contain the NRF2b (nfe212b) sequence. Users must order the complementary plasmid NRF2b (nfe212b)_R (OZ562) [Addgene plasmid 28087] in order to create a zinc finger nuclease (ZFN) pair to introduce targeted mutations into this specific zebrafish gene. Users will also need to clone the zinc finger (ZF) insert of this plasmid into a zinc finger nuclease (ZFN) vector to express a FOKI fusion product. Examples of possible ZFN expression vectors that can be used are: pST1374 (Addgene plasmid 13426), pMLM290 (Addgene plasmid 21872), pMLM292 (Addgene plasmid 21873), pMLM800 (Addgene plasmid 27202) and pMLM802 (Addgene plasmid 27203). This zinc finger array was tested for binding activity to the sequence 5'-GCCGGAGAC-3' in a bacterial two hybrid assay, and resulted in 6.02 fold activation. However, this array has not yet been tested for activity as a zinc finger nuclease (i.e. for its ability to induce mutations at the intended locus). Scientists using this zinc finger array in a publication should notify [email protected] and acknowledge NIH grant number R01 GM088040 in the publication. Other Articles: "Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays." Maeder ML et al. (Nat Protoc. 2009 Sept 17. 4(10):1471-1501. Pubmed ID: 19798082 "Targeted mutagenesis in zebrafish using customized zinc-finger nucleases." Foley JE et al. (Nat Protoc. 2009 Dec 3. 4(12):1855-1867. Pubmed ID: 20010934 Backbone Size:5493; Vector Backbone:MG414; Vector Types:Zebrafish Targeting; Bacterial Resistance:Kanamycin 2026-08-15 01:13:10 1
SI2-Unc5b
 
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RRID:Addgene_28215 Unc5b mouse monoclonal antibody Danio rerio Ampicillin PMID:20525357 See map and Genbank file for plasmid details. Backbone Marker:Yves Durocher, NRC; Backbone Size:5925; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:13:12 0
SI4-Jamc.2
 
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RRID:Addgene_28216 Jamc.2 mouse monoclonal antibody Danio rerio Ampicillin PMID:20525357 See map and Genbank file for plasmid details. Please note that Addgene's sequencing results identified nucleotide mismatches at bp# 3370-3371 and 3380-3382 when compared to the full plasmid sequence. These mismatches do not affect the production of recombinant antibodies, nor the affinity of the antibody for its antigen. Backbone Marker:Yves Durocher, NRC; Backbone Size:5925; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:13:11 1
pEMB44 / Zebrafish_LRRK2_1_338
 
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RRID:Addgene_40556 lrrk2 Danio rerio Ampicillin Backbone Marker:Emerald; Vector Backbone:pEMB44; Vector Types:Baculovirus; Bacterial Resistance:Ampicillin WT 2026-08-15 01:14:37 0
tol2-mpx-cxcr4b whim-gfp
 
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RRID:Addgene_41089 CXCR4b-WHIM Danio rerio Ampicillin PMID:20592249 Backbone Size:3000; Vector Backbone:tol2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin deleted last 19 amino acids of cxcr4b 2026-08-15 01:14:41 0
TAL3034
 
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RRID:Addgene_41208 ZebrafishCommunity-bsx-Left Danio rerio Ampicillin PMID:21822241 Target binding site: TCTCAGACTCACAGTTAT It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.3kb and 5.8kb. Backbone Marker:Addgene plasmid #32290; Backbone Size:6447; Vector Backbone:JDS78; Vector Types:Mammalian Expression, T7; Bacterial Resistance:Ampicillin 2026-08-15 01:14:43 1
TAL3033
 
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RRID:Addgene_41207 ZebrafishCommunity-bmp10-Right Danio rerio Ampicillin PMID:21822241 Target binding site: TCCATGTCCATCATCCAA It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.3kb and 5.8kb. Backbone Marker:Addgene plasmid #32285; Backbone Size:6447; Vector Backbone:JDS70; Vector Types:Mammalian Expression, T7; Bacterial Resistance:Ampicillin 2026-08-15 01:14:43 0
TAL3030
 
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RRID:Addgene_41204 ZebrafishCommunity-bbc3-(puma)-Left Danio rerio Ampicillin PMID:21822241 Target binding site: TGGCACGCCGAACAGCTG It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.3kb and 5.8kb. Backbone Marker:Addgene plasmid #32288; Backbone Size:6447; Vector Backbone:JDS74; Vector Types:Mammalian Expression, T7; Bacterial Resistance:Ampicillin 2026-08-15 01:14:42 0
TAL3027
 
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RRID:Addgene_41201 ZebrafishCommunity-arap3a-Right Danio rerio Ampicillin PMID:21822241 Target binding site: TGGATGGAAGCCAACAGG It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.3kb and 5.8kb. Backbone Marker:Addgene plasmid #32288; Backbone Size:6447; Vector Backbone:JDS74; Vector Types:Mammalian Expression, T7; Bacterial Resistance:Ampicillin 2026-08-15 01:14:42 0
TAL3082
 
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RRID:Addgene_41256 ZebrafishCommunity-gbas-Left Danio rerio Ampicillin PMID:21822241 Target binding site: TACCGGCAGCACAACCGT It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.3kb and 5.8kb. Backbone Marker:Addgene plasmid #32290; Backbone Size:6447; Vector Backbone:JDS78; Vector Types:Mammalian Expression, T7; Bacterial Resistance:Ampicillin 2026-08-15 01:14:43 0
TAL3078
 
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RRID:Addgene_41252 ZebrafishCommunity-gabrg2-Left Danio rerio Ampicillin PMID:21822241 Target binding site: TGGAAAGCGATGATGACG It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.3kb and 5.8kb. Backbone Marker:Addgene plasmid #32288; Backbone Size:6447; Vector Backbone:JDS74; Vector Types:Mammalian Expression, T7; Bacterial Resistance:Ampicillin 2026-08-15 01:14:43 0
TAL3077
 
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RRID:Addgene_41251 ZebrafishCommunity-gabra4-Right Danio rerio Ampicillin PMID:21822241 Target binding site: TTTTCCGGGTATAATTTC It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.3kb and 5.8kb. Backbone Marker:Addgene plasmid #32287; Backbone Size:6447; Vector Backbone:JDS71; Vector Types:Mammalian Expression, T7; Bacterial Resistance:Ampicillin 2026-08-15 01:14:43 0
TAL3076
 
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RRID:Addgene_41250 ZebrafishCommunity-gabra4-Left Danio rerio Ampicillin PMID:21822241 Target binding site: TAAAGAGAATATCTGGAC It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.3kb and 5.8kb. Backbone Marker:Addgene plasmid #32287; Backbone Size:6447; Vector Backbone:JDS71; Vector Types:Mammalian Expression, T7; Bacterial Resistance:Ampicillin 2026-08-15 01:14:43 0
TAL3075
 
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RRID:Addgene_41249 ZebrafishCommunity-fascin-2b-Right Danio rerio Ampicillin PMID:21822241 Target binding site: TGATTAATAAGCCCGAAT It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.3kb and 5.8kb. Backbone Marker:Addgene plasmid #32290; Backbone Size:6447; Vector Backbone:JDS78; Vector Types:Mammalian Expression, T7; Bacterial Resistance:Ampicillin 2026-08-15 01:14:43 0
TAL3070
 
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1+ mentions
RRID:Addgene_41244 ZebrafishCommunity-ets1a-Left Danio rerio Ampicillin PMID:21822241 Target binding site: TCAGAAGAGGAACATCAG It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.3kb and 5.8kb. Backbone Marker:Addgene plasmid #32288; Backbone Size:6447; Vector Backbone:JDS74; Vector Types:Mammalian Expression, T7; Bacterial Resistance:Ampicillin 2026-08-15 01:14:43 1
TAL3069
 
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RRID:Addgene_41243 ZebrafishCommunity-eml2-Right Danio rerio Ampicillin PMID:21822241 Target binding site: TTCTCCCTATTTTAAAAC It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.3kb and 5.8kb. Backbone Marker:Addgene plasmid #32287; Backbone Size:6447; Vector Backbone:JDS71; Vector Types:Mammalian Expression, T7; Bacterial Resistance:Ampicillin 2026-08-15 01:14:43 0
TAL3068
 
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RRID:Addgene_41242 ZebrafishCommunity-eml2-Left Danio rerio Ampicillin PMID:21822241 Target binding site: TCAGAAAAGTGGGAAGAC It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.3kb and 5.8kb. Backbone Marker:Addgene plasmid #32287; Backbone Size:6447; Vector Backbone:JDS71; Vector Types:Mammalian Expression, T7; Bacterial Resistance:Ampicillin 2026-08-15 01:14:43 0
TAL3067
 
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RRID:Addgene_41241 ZebrafishCommunity-elk1-Right Danio rerio Ampicillin PMID:21822241 Target binding site: TGCTTCTGATTCTGATCA It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.3kb and 5.8kb. Backbone Marker:Addgene plasmid #32285; Backbone Size:6447; Vector Backbone:JDS70; Vector Types:Mammalian Expression, T7; Bacterial Resistance:Ampicillin 2026-08-15 01:14:43 0
TAL3065
 
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RRID:Addgene_41239 ZebrafishCommunity-egfl7-Right Danio rerio Ampicillin PMID:21822241 Target binding site: TGAAGAAAAGACTCTGTG It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.3kb and 5.8kb. Backbone Marker:Addgene plasmid #32288; Backbone Size:6447; Vector Backbone:JDS74; Vector Types:Mammalian Expression, T7; Bacterial Resistance:Ampicillin 2026-08-15 01:14:43 0
TAL3062
 
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RRID:Addgene_41236 ZebrafishCommunity-ednrb1b-Left Danio rerio Ampicillin PMID:21822241 Target binding site: TACATCAGCAGCGTGGTG It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.3kb and 5.8kb. Backbone Marker:Addgene plasmid #32288; Backbone Size:6447; Vector Backbone:JDS74; Vector Types:Mammalian Expression, T7; Bacterial Resistance:Ampicillin 2026-08-15 01:14:43 0

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