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Species: Bos taurus
Genetic Insert: YFP(159-238)-gamma-1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4800; Vector Backbone:pcDNAI/Amp; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15136579
Proper citation: RRID:Addgene_54471 Copy
Species: Synthetic
Genetic Insert: SRM (sortase-recognition motif)
Vector Backbone Description: Backbone Size:2513; Vector Backbone:pFA6a; Vector Types:yeast genome targeting; Bacterial Resistance:Ampicillin
Comments: Based on the plasmids from Mark Hochstrasser for epitope tagging in S. cerevisiae:
http://www.addgene.org/browse/article/2501/
sortase sequence = LPETG
full sortase recognition motif = GSLPETGGGRR
Proper citation: RRID:Addgene_54489 Copy
Species: Synthetic
Genetic Insert: SRM (sortase-recognition motif)
Vector Backbone Description: Backbone Size:2513; Vector Backbone:pFA6a; Vector Types:yeast genome targeting; Bacterial Resistance:Ampicillin
Comments: Based on the plasmids from Mark Hochstrasser for epitope tagging in S. cerevisiae:
http://www.addgene.org/browse/article/2501/
sortase sequence = LPETG
full sortase recognition motif = GSLPETGGGRR
Proper citation: RRID:Addgene_54488 Copy
Vector Backbone Description: Backbone Size:4800; Vector Backbone:mOrange2-pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18454154
Comments: Contains 6x-Histidine tag for protein purification. Excitation = 549; Emission = 565
Proper citation: RRID:Addgene_54531 Copy
Vector Backbone Description: Backbone Size:4800; Vector Backbone:EBFP2-pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Contains 6x-Histidine tag for protein purification. Excitation = 383; Emission = 448
Proper citation: RRID:Addgene_54542 Copy
Vector Backbone Description: Backbone Size:4800; Vector Backbone:mKO2-pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18267078
Comments: Contains 6x-Histidine tag for protein purification. Excitation = 551; Emission = 565
Proper citation: RRID:Addgene_54555 Copy
Vector Backbone Description: Backbone Size:4800; Vector Backbone:mTFP1-pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16859491
Comments: Contains 6x-Histidine tag for protein purification. Excitation = 462; Emission = 492
Proper citation: RRID:Addgene_54553 Copy
Vector Backbone Description: Backbone Size:4800; Vector Backbone:DsRed2-pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Contains 6x-Histidine tag for protein purification. Excitation = 558; Emission = 583
Proper citation: RRID:Addgene_54608 Copy
Vector Backbone Description: Backbone Size:4800; Vector Backbone:Clover-pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22961245
Comments: Contains 6x-Histidine tag for protein purification. Excitation = 505; Emission = 515
Proper citation: RRID:Addgene_54575 Copy
Vector Backbone Description: Backbone Size:4800; Vector Backbone:mTagBFP2-pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22174863
Comments: Contains 6x-Histidine tag for protein purification. Excitation = 399; Emission = 456
Proper citation: RRID:Addgene_54572 Copy
Vector Backbone Description: Backbone Size:4800; Vector Backbone:mT-Sapphire-pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18425137
Comments: Contains 6x-Histidine tag for protein purification. Excitation = 399; Emission = 511
Proper citation: RRID:Addgene_54571 Copy
Vector Backbone Description: Backbone Marker:Phil Soriano, Addgene Plasmid #21714; Vector Backbone:ROSA26-1; Vector Types:Mammalian Expression, Mouse Targeting, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24166816
Comments: Unique HpaI and XhoI sites were maintained for single step short hairpin oligonucleotide cloning.
Using the primers described above yields 231bp and 177bp for positive and negative products, respectively.
Proper citation: RRID:Addgene_54479 Copy
Vector Backbone Description: Backbone Size:4800; Vector Backbone:mCerulean-pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21479270
Comments: Contains 6x-Histidine tag for protein purification. Excitation = 433; Emission = 475
Proper citation: RRID:Addgene_54666 Copy
Vector Backbone Description: Backbone Size:4800; Vector Backbone:mRFP1-pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12060735
Comments: Contains 6x-Histidine tag for protein purification. Excitation = 584; Emission = 607
Proper citation: RRID:Addgene_54667 Copy
Vector Backbone Description: Backbone Size:4800; Vector Backbone:mEGFP-pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Contains 6x-Histidine tag for protein purification. Excitation = 488; Emission = 507
Proper citation: RRID:Addgene_54622 Copy
Vector Backbone Description: Backbone Size:4800; Vector Backbone:mCherry-pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15558047
Comments: Contains 6x-Histidine tag for protein purification. Excitation = 587; Emission = 610
Proper citation: RRID:Addgene_54630 Copy
Vector Backbone Description: Backbone Size:4800; Vector Backbone:mRuby-pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Contains 6x-Histidine tag for protein purification. Excitation = 558; Emission = 605.
Addgene sequencing finds 1 mismatch to depositor's sequence(G211C), which causes L31F aa change. This is consistent with other isoforms of mRuby.
Proper citation: RRID:Addgene_54763 Copy
Vector Backbone Description: Backbone Size:4800; Vector Backbone:mCardinal-pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24633408
Comments: Contains 6x-Histidine tag for protein purification. Excitation = 604; Emission = 659
Proper citation: RRID:Addgene_54800 Copy
Vector Backbone Description: Backbone Size:4800; Vector Backbone:Emerald-pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Contains 6x-Histidine tag for protein purification. Excitation = 487; Emission = 509
Proper citation: RRID:Addgene_54776 Copy
Vector Backbone Description: Backbone Size:4800; Vector Backbone:mRuby2-pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22961245
Comments: Contains 6x-Histidine tag for protein purification. Excitation = 559; Emission = 600
Proper citation: RRID:Addgene_54771 Copy
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