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Species: Saccharomyces cerevisiae
Genetic Insert: Booster-ROCK
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32101394
Comments: Sequences are based on next-generation sequencing (NGS) results where indicated (Addgene NGS Result), or assembled from reference sequences and/or Sanger results (Addgene Assembled Sequence). Sequence provided by depositing laboratory may be theoretical/predicted or based on Sanger/NGS sequencing results. Discrepancies between sequencing results obtained by Addgene and the original sequence provided by the depositor may be present.
Proper citation: RRID:Addgene_138377 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GCN4
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_138620 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: TY5
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_138622 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: CBF1
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_138616 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: TYE7
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_138617 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GCR2
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_138619 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: mutant HSP104
Vector Backbone Description: Backbone Size:0; Vector Backbone:pJC25S; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11158570
Proper citation: RRID:Addgene_1300 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Cdc20/mCherry-AID(71-114)-tCYC1-NatMX
Vector Backbone Description: Backbone Marker:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC55758/; Backbone Size:2531; Vector Backbone:HO-poly-KanMX4-HO; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31606371
Comments: This plasmid can be used to dynamically and conditionally deplete Cdc20 in yeast.
The work is currently under review.
Proper citation: RRID:Addgene_130270 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Hsp104 Y257W
Vector Backbone Description: Backbone Size:2375; Vector Backbone:pJC45; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11983167
Proper citation: RRID:Addgene_1303 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Hsp104 L892W
Vector Backbone Description: Backbone Size:2375; Vector Backbone:pJC45; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11983167
Proper citation: RRID:Addgene_1308 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Hsp104 Y257W
Vector Backbone Description: Backbone Size:5586; Vector Backbone:pGAL; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11983167
Proper citation: RRID:Addgene_1310 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Hsp104 Y466W
Vector Backbone Description: Backbone Size:5586; Vector Backbone:pGAL; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11983167
Proper citation: RRID:Addgene_1311 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Cdc3 + Cdc10-SNAP
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pACYC-Duet1; Vector Types:Bacterial Expression, bicistronic; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:23889817
Comments: combine with pET-Cdc12-Cdc11 for expression of SNAP-tagged septin rods
Proper citation: RRID:Addgene_131161 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Hsp104 Y507W
Vector Backbone Description: Backbone Size:5586; Vector Backbone:pGAL; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11983167
Proper citation: RRID:Addgene_1312 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RPL11B
Vector Backbone Description: Backbone Size:0; Vector Backbone:pPS1530; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11071906
Comments: CEN vector with ORF of GFP followed by the 3'UTR of NUF2.
Proper citation: RRID:Addgene_1364 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RPL25-U1A-ASH1 3' UTR
Vector Backbone Description: Backbone Size:5726; Vector Backbone:pRS426; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11142374
Comments: RPL25 promoter and RPL25 ORF (XhoI/BamHI fragment), U1A hairpins (BamHI/BglII fragment), ASH1 3' UTR (SpeI/NotI fragment), ADH2 terminator (NotI/SacII fragment), 2 micron plasmid.
Proper citation: RRID:Addgene_1369 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: PGK1-U1A-PGK1 3' UTR
Vector Backbone Description: Backbone Size:5726; Vector Backbone:pRS426; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11142374
Comments: PGK1 promoter and PGK1 ORF (XhoI/BamHI fragment), U1A hairpins (BamHI/BglII fragment), PGK1 3' UTR (SpeI/NotI fragment), ADH2 terminator (NotI/SacII fragment), 2 micron plasmid.
Proper citation: RRID:Addgene_1370 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SSA4-U1A-ASH1 3' UTR
Vector Backbone Description: Backbone Size:5726; Vector Backbone:pRS426; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11142374
Comments: SSA4 promoter and SSA4 ORF (XhoI/BamHI fragment), U1A hairpins (BamHI/BglII fragment), ASH1 3' UTR (SpeI/NotI fragment), ADH2 terminator (NotI/SacII fragment), 2 micron plasmid.
Proper citation: RRID:Addgene_1371 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: PGK1-U1A-ASH1 3' UTR
Vector Backbone Description: Backbone Size:5726; Vector Backbone:pRS426; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11142374
Comments: PGK1 promoter and PGK1 ORF (XhoI/BamHI fragment), U1A hairpins (BamHI/BglII fragment), ASH1 3' UTR (SpeI/NotI fragment), ADH2 terminator (NotI/SacII fragment), 2 micron plasmid.
Proper citation: RRID:Addgene_1372 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Signal recognition particle receptor β subunit
Vector Backbone Description: Backbone Size:5332; Vector Backbone:pET28a-derived; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16627619
Proper citation: RRID:Addgene_13729 Copy
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