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Species: Caenorhabditis elegans
Genetic Insert: beta-tubulin
Vector Backbone Description: Backbone Size:0; Vector Backbone:pie-1; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18692039
Comments: 1st generation pie-1 vector
Proper citation: RRID:Addgene_26951 Copy
Species: Caenorhabditis elegans
Genetic Insert: TRA-2 GFP
Vector Backbone Description: Backbone Size:2700; Vector Backbone:pPD95.79; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20921392
Proper citation: RRID:Addgene_27128 Copy
Species: Caenorhabditis elegans
Genetic Insert: TRA-2 FLAG
Vector Backbone Description: Backbone Size:2700; Vector Backbone:pPD95.79; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20921392
Comments: Cloning of this plasmid was achieved in the following steps:
(1)the sequence 3' (3'UTR) of the tags was added by (EcoRI/SpeI)
(2) 1.2 kb XhoI/KpnI tra-2 pcr fragment into SalI/KpnI cut vector (destroying one SalI/XhoI site but adding another in the insert) immediately 5' of the tag
(3) 7 kb HindIII/SalI fragment into same that partially replaced fragment added in step 2 containing most of the TRA-2 coding sequence
(4) 5 kb Xho/AgeI that added the promoter and upstream gene in the operon as well as some Tra-2 coding sequence
Proper citation: RRID:Addgene_27129 Copy
Species: Caenorhabditis elegans
Genetic Insert: Thr412Gly-CePheRS Alpha Subunit
Vector Backbone Description: Backbone Marker:Andrew Fire (Addgene Plasmid #1494) ; Backbone Size:4494; Vector Backbone:pPD95.75; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25691744
Proper citation: RRID:Addgene_62599 Copy
Species: Caenorhabditis elegans
Genetic Insert: MBP-PGL-1
Vector Backbone Description: Backbone Size:8356; Vector Backbone:pMAL-c2X Gateway compatible destination vector; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25535836
Proper citation: RRID:Addgene_62867 Copy
Species: Caenorhabditis elegans
Genetic Insert: MBP-PPTR-2
Vector Backbone Description: Backbone Size:8356; Vector Backbone:pMAL-c2X Gateway compatible destination vector; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25535836
Proper citation: RRID:Addgene_62869 Copy
Species: Caenorhabditis elegans
Genetic Insert: PAA-1/Y3H
Vector Backbone Description: Backbone Size:8128; Vector Backbone:pY3H Gateway compatible destination vector; Vector Types:Yeast Expression, two-hybrid bridge vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25535836
Proper citation: RRID:Addgene_62865 Copy
Species: Caenorhabditis elegans
Genetic Insert: GST-MEG-1
Vector Backbone Description: Backbone Size:4984; Vector Backbone:pGEX6p1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25535836
Proper citation: RRID:Addgene_62879 Copy
Species: Caenorhabditis elegans
Genetic Insert: MBP-MEG-1
Vector Backbone Description: Backbone Size:8356; Vector Backbone:pMAL-c2X Gateway compatible destination vector; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25535836
Proper citation: RRID:Addgene_62875 Copy
Species: Caenorhabditis elegans
Genetic Insert: MBP-MEG-3
Vector Backbone Description: Backbone Size:8356; Vector Backbone:pMAL-c2X Gateway compatible destination vector; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25535836
Proper citation: RRID:Addgene_62876 Copy
Species: Caenorhabditis elegans
Genetic Insert: MBP-MBK-2 K196R
Vector Backbone Description: Backbone Size:8356; Vector Backbone:pMAL-c2X Gateway compatible destination vector; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25535836
Proper citation: RRID:Addgene_62872 Copy
Species: Caenorhabditis elegans
Genetic Insert: GST-MEG-4
Vector Backbone Description: Backbone Size:4984; Vector Backbone:pGEX6p1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25535836
Proper citation: RRID:Addgene_62881 Copy
Species: Caenorhabditis elegans
Genetic Insert: GFP::MEG-3
Vector Backbone Description: Backbone Marker:Seydoux lab, Addgene plasmid # 26955; Backbone Size:8260; Vector Backbone:pID2.02; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25535836
Comments: InFusion cloning
Proper citation: RRID:Addgene_62882 Copy
Species: Caenorhabditis elegans
Genetic Insert: Tc3A
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET3C; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15304566
Proper citation: RRID:Addgene_63138 Copy
Species: Caenorhabditis elegans
Genetic Insert: Thr412Gly-CePheRS Alpha Subunit::C. elegans CEOP5428 Intercistronic Region::HA-GFP [From pPD95.75 (+118 to +1000)] in pDONR221
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2544; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Comments: Note: the first methionine in the alpha subunit of C. elegans PheRS, Isoform A, Exon 3 is designated as residue 1 (“Met1”).
Proper citation: RRID:Addgene_72673 Copy
Species: Caenorhabditis elegans
Genetic Insert: let-858 3' UTR [From pPD129.57 (+2554 to +2960)] in pDONR P2R-P3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2639; Vector Backbone:pDONR P2R-P3; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_72674 Copy
Species: Caenorhabditis elegans
Genetic Insert: mTurquoise2-C1^SEC^3xFlag
Vector Backbone Description: Backbone Size:2600; Vector Backbone:pUC19 (modified); Vector Types:Worm Expression, Cre/Lox, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37351566
Comments: pDD315 is a derivative of our published vectors pDD282-285 (Dickinson et al. Genetics 2015). It has a 2xHA tag in place of 3xFlag, and Lox511I sites in place of LoxP. These features allow pDD315 to be used in a genetic background that has already been modified using a green or red FP-SEC vector, without conflicts between epitope tags and Lox sites.
Proper citation: RRID:Addgene_73343 Copy
Species: Caenorhabditis elegans
Genetic Insert: Pmyo-2::GFP + SEC
Vector Backbone Description: Backbone Size:2600; Vector Backbone:pUC19 (modified); Vector Types:Worm Expression, Cre/Lox, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34903234
Comments: pDD317 is a derivative of our published vectors pDD282-285 (Dickinson et al. Genetics 2015) and is intended for making gene knockouts. It has a Pmyo-2::GFP expression cassette (bright pharyngeal fluorescence) in place of the simple fluorescent protein found in our other FP–SEC vectors. Pmyo-2::GFP + SEC is intended to be inserted in place of an entire ORF such that after insertion and SEC removal, the knockout is marked with Pmyo-2::GFP. The vector also has Lox511I sites in place of LoxP, allowing pDD315 to be used in a genetic background that has already been modified using a green or red FP-SEC vector, without conflicts between Lox sites.
Proper citation: RRID:Addgene_73344 Copy
Species: Caenorhabditis elegans
Genetic Insert: unc-70::TSMod
Vector Backbone Description: Vector Backbone:pEXPR; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24561618
Proper citation: RRID:Addgene_73367 Copy
Species: Caenorhabditis elegans
Genetic Insert: pmex-5::mKate2::PH::tbb-2
Vector Backbone Description: Vector Backbone:modified pCFJ150; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27385332
Proper citation: RRID:Addgene_73512 Copy
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