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Species: Caenorhabditis elegans
Genetic Insert: him-3 ORF+3' in pDONR201
Vector Backbone Description: Backbone Size:2257; Vector Backbone:pDONR201; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_19366 Copy
Species: Caenorhabditis elegans
Genetic Insert: nos-3 ORF+3' in pDONR201
Vector Backbone Description: Backbone Size:2257; Vector Backbone:pDONR201; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_19403 Copy
Species: Caenorhabditis elegans
Genetic Insert: lip-1 ORF+3' in pDONR201
Vector Backbone Description: Backbone Size:2257; Vector Backbone:pDONR201; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_19402 Copy
Species: Caenorhabditis elegans
Genetic Insert: cxTi10882 targetting region
Vector Backbone Description: Backbone Size:2448; Vector Backbone:N/A; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18953339
Comments: Standard Multiple Cloning site vector for targeted insertions at cxTi10882 on Chr. IV.
Contains C.Briggsae unc-119 rescue fragment.
Proper citation: RRID:Addgene_19331 Copy
Species: Caenorhabditis elegans
Genetic Insert: Pglh-2::Mos1 transposase
Vector Backbone Description: Backbone Size:2961; Vector Backbone:N/A; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18953339
Comments: This plasmid was made by Dr. Jean Louis Bessereau and described in the publication: Bessereau JL, Wright A, Williams DC, Schuske K, Davis MW, Jorgensen EM.
Mobilization of a Drosophila transposon in the Caenorhabditis elegans germ line.
Nature. 2001 Sep 6;413(6851):70-4.
Proper citation: RRID:Addgene_19332 Copy
Species: Caenorhabditis elegans
Genetic Insert: par-5 3'utr in pDONRP2RP3
Vector Backbone Description: Backbone Size:2641; Vector Backbone:pDONRP2RP3; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_19398 Copy
Species: Caenorhabditis elegans
Genetic Insert: Punc-122::GFP
Vector Backbone Description: Backbone Size:2448; Vector Backbone:N/A; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18953339
Comments: Use this plasmid as a positive control for insertion frequency by MosSCI at ttTi5605 locus. Correct insertion will give dim (not visible on dissection scope) fluorescence in the coelomycytes.
Proper citation: RRID:Addgene_19325 Copy
Species: Caenorhabditis elegans
Genetic Insert: pDESTttTi5605[R4-R3]
Vector Backbone Description: Backbone Size:2354; Vector Backbone:N/A; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18953339
Comments: Invitrogen Gateway 3-fragment compatible destination vector. Contains C.Briggsae unc-119 rescue fragment. Also contains genomic regions flanking ttTi5605 Mos1 insertion to generate MosSCI inserts at this locus.
Please note that due to a bug in the Addgene map algorithm, the attR sites are depicted as attR1 and attR2, when in fact they are attR3 and attR4. Please refer to the author's map for an accurate depiction of these sites.
Proper citation: RRID:Addgene_19329 Copy
Species: Caenorhabditis elegans
Genetic Insert: him-3 3'utr in pDONRP2RP3
Vector Backbone Description: Backbone Size:2641; Vector Backbone:pDONRP2RP3; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_19381 Copy
Species: Caenorhabditis elegans
Genetic Insert: spn-4 3'utr in pDONRP2RP3
Vector Backbone Description: Backbone Size:2641; Vector Backbone:pDONRP2RP3; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_19383 Copy
Species: Caenorhabditis elegans
Genetic Insert: spe-38 3'utr in pDONRP2RP3
Vector Backbone Description: Backbone Size:2641; Vector Backbone:pDONRP2RP3; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_19375 Copy
Species: Caenorhabditis elegans
Genetic Insert: fog-1 3'utr in pDONRP2RP3
Vector Backbone Description: Backbone Size:2641; Vector Backbone:pDONRP2RP3; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_19374 Copy
Species: Caenorhabditis elegans
Genetic Insert: nos-3 3'utr in pDONRP2RP3
Vector Backbone Description: Backbone Size:2641; Vector Backbone:pDONRP2RP3; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_19395 Copy
Species: Caenorhabditis elegans
Genetic Insert: gld-1 3'utr in pDONRP2RP3
Vector Backbone Description: Backbone Size:2641; Vector Backbone:pDONRP2RP3; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_19394 Copy
Species: Caenorhabditis elegans
Genetic Insert: mex-5 3'utr in pDONRP2RP3
Vector Backbone Description: Backbone Size:2641; Vector Backbone:pDONRP2RP3; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_19385 Copy
Species: Caenorhabditis elegans
Genetic Insert: mex-3 3'utr in pDONRP2RP3
Vector Backbone Description: Backbone Size:2641; Vector Backbone:pDONRP2RP3; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_19387 Copy
Species: Caenorhabditis elegans
Genetic Insert: Gateway(R1-R2)<4xNLS-GFP::let-858(3’)-(Cbr)unc-119(+)<4xNLS-tdimer2(12)::let-858(3’)
Vector Backbone Description: Backbone Size:0; Vector Backbone:pGC91; Vector Types:Worm Expression, FLP/FRT, Destination vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18723890
Comments: Sequence mismatches between Addgene sequence and depositor sequence do not affect function.
Proper citation: RRID:Addgene_19630 Copy
Species: Caenorhabditis elegans
Genetic Insert: Gateway(R1-R2)
Defining Citation: PMID:18723890
Comments: Sequence mismatches between Addgene sequence and depositor sequence do not affect function.
Proper citation: RRID:Addgene_19632 Copy
Species: Caenorhabditis elegans
Genetic Insert: mex-5 prom in PDONRP4P1R
Vector Backbone Description: Backbone Size:2645; Vector Backbone:PDONRP4P1R; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_19598 Copy
Species: Caenorhabditis elegans
Genetic Insert: Gateway(R1-R2)
Defining Citation: PMID:18723890
Proper citation: RRID:Addgene_19631 Copy
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