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Species: Other
Genetic Insert: GUT1 promoter
Vector Backbone Description: Backbone Size:1936; Vector Backbone:BB1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29221460
Proper citation: RRID:Addgene_98511 Copy
Species: Other
Genetic Insert: FDH1 promoter
Vector Backbone Description: Backbone Size:1936; Vector Backbone:BB1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29221460
Proper citation: RRID:Addgene_98516 Copy
Species: Other
Genetic Insert: ScCYC1tt
Vector Backbone Description: Backbone Size:1936; Vector Backbone:BB1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29221460
Proper citation: RRID:Addgene_98519 Copy
Species: Other
Genetic Insert: RPS2tt
Vector Backbone Description: Backbone Size:1936; Vector Backbone:BB1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29221460
Proper citation: RRID:Addgene_98521 Copy
Species: Other
Genetic Insert: RPS17Btt
Vector Backbone Description: Backbone Size:1936; Vector Backbone:BB1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29221460
Proper citation: RRID:Addgene_98523 Copy
Species: Other
Genetic Insert: chr4_0883tt
Vector Backbone Description: Backbone Size:1936; Vector Backbone:BB1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29221460
Proper citation: RRID:Addgene_98524 Copy
Species: Other
Genetic Insert: RPS3tt
Vector Backbone Description: Backbone Size:1936; Vector Backbone:BB1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29221460
Proper citation: RRID:Addgene_98527 Copy
Species: Other
Genetic Insert: RPS25Att
Vector Backbone Description: Backbone Size:1936; Vector Backbone:BB1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29221460
Proper citation: RRID:Addgene_98526 Copy
Species: Other
Genetic Insert: mEos4b-V69T
Vector Backbone Description: Vector Backbone:pRsetA; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28574633
Comments: mEos4b-V69T is named and aligned in reference to the Dendra2 fluorescent protein sequence. The mutation in the mEos4b reference is at position 70. However, to avoid confusion, we named this protein in alignment with Dendra2 (see Fig. S1a, 10.1002/anie.201702870).
Proper citation: RRID:Addgene_98572 Copy
Species: Other
Genetic Insert: Cas9
Vector Backbone Description: Vector Backbone:pMZ374 (Addgene 59896); Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29703785
Comments: These plasmids are used for a cloning-free method of CRISPR/Cas9-mediated genome editing in fission yeast. This method takes advantage of gap repair in fission yeast cells to assemble two linear DNA fragments, a gapped Cas9-encoding plasmid and a PCR-amplified gRNA insert, into a circular plasmid. To reduce non-specific gap repair background, we adopt a split-marker strategy. pDB4280 and pDB4282 serve as the source of the gapped plasmid and the PCR template for gRNA insert amplification, respectively, in the split-ura4 system. pDB4281 and pDB4283 serve the same purposes for the split-bsdMX system. pDB4279 is a transformation control for the split-bsdMX system.
Proper citation: RRID:Addgene_98699 Copy
Species: Other
Genetic Insert: mMaple3-V157I
Vector Backbone Description: Vector Backbone:pRsetA; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28574633
Comments: mMaple3-V157I is named and aligned in reference to the Dendra2 fluorescent protein sequence. The mutation in the mMaple3 reference is at position 166. However, to avoid confusion, we named this protein in alignment with Dendra2 (see Fig. S1a, 10.1002/anie.201702870).
Proper citation: RRID:Addgene_98577 Copy
Species: Other
Genetic Insert: Cas9
Vector Backbone Description: Vector Backbone:pMZ374 (Addgene 59896); Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29703785
Comments: These plasmids are used for a cloning-free method of CRISPR/Cas9-mediated genome editing in fission yeast. This method takes advantage of gap repair in fission yeast cells to assemble two linear DNA fragments, a gapped Cas9-encoding plasmid and a PCR-amplified gRNA insert, into a circular plasmid. To reduce non-specific gap repair background, we adopt a split-marker strategy. pDB4280 and pDB4282 serve as the source of the gapped plasmid and the PCR template for gRNA insert amplification, respectively, in the split-ura4 system. pDB4281 and pDB4283 serve the same purposes for the split-bsdMX system. pDB4279 is a transformation control for the split-bsdMX system.
Proper citation: RRID:Addgene_98700 Copy
Species: Other
Genetic Insert: ArchT-GFP
Vector Backbone Description: Backbone Marker:Scott Sternson; Vector Backbone:AAV with CaMKII promoter; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21811444
Proper citation: RRID:Addgene_99039 Copy
Species: Other
Genetic Insert: eyes absent (eya)
Vector Backbone Description: Backbone Marker:Phillip Newmark (Addgene plasmid # 26536); Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23297191
Proper citation: RRID:Addgene_99085 Copy
Species: Other
Genetic Insert: prohormone convertase 2 (PC2)
Vector Backbone Description: Backbone Marker:Phillip Newmark (Addgene plasmid # 26536); Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23297191
Comments: Cloning primers: F-TCGTTGCACACCTGTACCAT, R-CACTCCAACACCACAAATGC
Proper citation: RRID:Addgene_99087 Copy
Species: Other
Genetic Insert: Dimeric MS2-coat binding protein
Vector Backbone Description: Backbone Size:10000; Vector Backbone:pHR; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28562589
Proper citation: RRID:Addgene_99151 Copy
Species: Other
Genetic Insert: mNeonGreen
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28671695
Proper citation: RRID:Addgene_99131 Copy
Species: Other
Genetic Insert: CmYLCV
Vector Backbone Description: Vector Backbone:plCH41295; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:36253612
Comments: MoClo compatible
Proper citation: RRID:Addgene_176872 Copy
Species: Other
Genetic Insert: E2-Crimson-NLS
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28764932
Comments: Please note: This plasmid contains only one ITR. This is not known to affect plasmid function.
Proper citation: RRID:Addgene_183803 Copy
Species: Other
Genetic Insert: E2-Crimson-NLS
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28764932
Comments: Please note: This plasmid contains only one ITR. This is not known to affect plasmid function.
Proper citation: RRID:Addgene_183801 Copy
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