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Species: Rattus norvegicus
Genetic Insert: Calcium/Calmodulin dependent protein kinase kinase beta
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4100; Vector Backbone:pSG5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18460329
Comments: The S3V, G96R, and P108L "mutations" were present in the original version of rat CaMKKbeta cloned by the Means lab. They believe these sequence differences represent a natural variant of the CaMKKbeta sequence and are not mutations.
Proper citation: RRID:Addgene_32452 Copy
Species: Rattus norvegicus
Genetic Insert: Calcium/Calmodulin dependent protein kinase kinase beta
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4100; Vector Backbone:pSG5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18460329
Comments: The S3V, G96R, and P108L "mutations" were present in the original version of rat CaMKKbeta cloned by the Means lab. They believe these sequence differences represent a natural variant of the CaMKKbeta sequence and are not mutations.
Proper citation: RRID:Addgene_32450 Copy
Species: Rattus norvegicus
Genetic Insert: Calcium/Calmodulin dependent protein kinase kinase beta
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4100; Vector Backbone:pSG5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18460329
Comments: The S3L, G96R, and P108L "mutations" were present in the original version of rat CaMKKbeta cloned by the Means lab. They believe these sequence differences represent a natural variant of the CaMKKbeta sequence and are not mutations.
Proper citation: RRID:Addgene_32449 Copy
Species: Rattus norvegicus
Genetic Insert: dominant negative Rheb (D60K)
Vector Backbone Description: Backbone Marker:Addgene plasmid #32519; Vector Backbone:pHAGE-CMV-Rheb-IRES-eGFP-W; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Comments: The published dominant negative D60K mutation was introduced to pHAGE-CMV-Rheb-IRES-eGFP-W by site directed mutagenesis. Although reported to have robust DN activity in other cell types, this construct did not provide robust DN activity in neurons.
The original pHAGE-CMV-IRES lentiviral vector is from Dr Richard C. Mulligan at Harvard (Mostoslavsky G et al., 2005).
Proper citation: RRID:Addgene_32522 Copy
Species: Rattus norvegicus
Genetic Insert: Dominant negative Rheb (D60I)
Vector Backbone Description: Backbone Marker:Addgene plasmid #32519; Vector Backbone:pHAGE-CMV-Rheb-IRES-eGFP-W; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Comments: The published dominant negative D60I mutation was introduced to pHAGE-CMV-Rheb-IRES-eGFP-W by site directed mutagenesis. Although reported to have robust DN activity in other cell types, this construct did not provide robust DN activity in neurons.
The original pHAGE-CMV-IRES lentiviral vector is from Dr Richard C. Mulligan at Harvard (Mostoslavsky G et al., 2005).
Proper citation: RRID:Addgene_32521 Copy
Species: Rattus norvegicus
Genetic Insert: Rheb
Vector Backbone Description: Backbone Size:8450; Vector Backbone:pHAGE-CMV-eGFP-IRES-eGFP-W; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20062052
Comments: Cloning of Rheb into the NotI and BamHI sites of pHAGE-CMV-eGFP-IRES-eGFP-W replaces the first copy of eGFP.
The original pHAGE-CMV-IRES lentiviral vector is from Dr Richard C. Mulligan at Harvard (Mostoslavsky G et al., 2005).
Proper citation: RRID:Addgene_32519 Copy
Species: Rattus norvegicus
Genetic Insert: TRKB
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_32500 Copy
Species: Rattus norvegicus
Genetic Insert: Dynamin I
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12011079
Proper citation: RRID:Addgene_32695 Copy
Species: Rattus norvegicus
Genetic Insert: Dynamin I
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12011079
Proper citation: RRID:Addgene_32696 Copy
Species: Rattus norvegicus
Genetic Insert: Mef2d
Vector Backbone Description: Backbone Marker:OligoEngine; Backbone Size:3176; Vector Backbone:pSuper; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16484497
Comments: Can be used in conjunction with pcDNA3-rMEF2D (Addgene #32902) which is mutated so as to not be targeted by this shRNA.
Proper citation: RRID:Addgene_32900 Copy
Species: Rattus norvegicus
Genetic Insert: β-arrestin 1 S412A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9924018
Comments: Flag epitope-tagged truncation mutants of β-arrestin 1 were prepared by the polymerase chain reaction (PCR), incorporating an Eco RI site, minimal Kozak sequence (ACC), and initiator methionine codon into the 5' primer, and the Flag epitope sequence, stop codon, and an Xho I site into the 3' primer. PCR products were subcloned into pcDNA3. DNA sequences were confirmed by dideoxynucleotide sequencing.
Proper citation: RRID:Addgene_42195 Copy
Species: Rattus norvegicus
Genetic Insert: Gamma 5
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22538369
Proper citation: RRID:Addgene_42200 Copy
Species: Rattus norvegicus
Genetic Insert: eIF4E
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pLJM60; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22552098
Proper citation: RRID:Addgene_38239 Copy
Species: Rattus norvegicus
Genetic Insert: tsc2
Vector Backbone Description: Vector Backbone:pGEX-KG; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_38107 Copy
Species: Rattus norvegicus
Genetic Insert: Cytochrome b5
Vector Backbone Description: Backbone Marker:Dr. Toshio Kitamura of the University of Tokyo ; Backbone Size:6878; Vector Backbone:pMXs-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20639694
Proper citation: RRID:Addgene_38274 Copy
Species: Rattus norvegicus
Genetic Insert: β-arrestin2
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pCDNA 3.1 zeo-; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18534983
Proper citation: RRID:Addgene_38262 Copy
Species: Rattus norvegicus
Genetic Insert: Wnk1
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10828064
Proper citation: RRID:Addgene_38780 Copy
Species: Rattus norvegicus
Genetic Insert: Wnk1
Vector Backbone Description: Backbone Size:4800; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10828064
Comments: Low level of expression
Proper citation: RRID:Addgene_38779 Copy
Species: Rattus norvegicus
Genetic Insert: Omp25
Vector Backbone Description: Backbone Marker:Dr. Toshio Kitamura of the University of Tokyo ; Backbone Size:5800; Vector Backbone:pMXs-IP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21454557
Proper citation: RRID:Addgene_38249 Copy
Species: Rattus norvegicus
Genetic Insert: Erk2
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:5000; Vector Backbone:p3xFlag-CMV7.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11823456
Proper citation: RRID:Addgene_39223 Copy
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